The welfare and healthy growth of poultry under intensive feeding conditions are closely related to their living environment. In spring, the air quality considerably decreases due to reduced ventilation and aeration in cage systems, which influences the meat quality and health of broilers during normal growth stages. In this study, we analyzed the airborne bacterial communities in PM2.5 and PM10 in cage broiler houses at different broiler growth stages under intensive rearing conditions based on the high-throughput 16S rDNA sequencing technique. Our results revealed that PM2.5, PM10 and airborne microbes gradually increased during the broiler growth cycle in poultry houses. Some potential or opportunistic pathogens, including Acinetobacter, Pseudomonas, Enterococcus, Microbacterium, etc., were found in the broiler houses at different growth stages. Our study evaluated variations in the microbial communities in PM2.5 and PM10 and potential opportunistic pathogens during the growth cycle of broilers in poultry houses in the spring. Our findings may provide a basis for developing technologies for air quality control in caged poultry houses.
Heterogeneous nuclear ribonucleoprotein K (hnRNP K), is a multifunctional protein that participates in a variety of regulatory processes of signal transduction and gene expression. To further characterize the significance of hnRNP K in different male germ cells, we investigated the expression profiles of hnRNP K at different developmental stages in pig and rat testes, and conducted a comparative analysis of expression patterns between these two species. In porcine testis development, both the mRNA and protein level of hnRNP K were down-regulated from 3 months to 8 months. However, the expression level of hnRNP K was abundant across the embryonic period in rats, and decreased gradually from 0 day post partum (dpp) to 14 dpp, then increased with the highest level presenting at 90 dpp. Immunolocalization analysis further confirmed the differential expression and localization of hnRNP K protein during testis development in pigs and rats. The results showed that hnRNP K was widely distributed in gonocytes, spermatogonia, sertoli cells and Leydig cells. The dynamic expression profile of hnRNP K may imply its crucial and potential roles in the development of the testis, which will provide a theoretical basis for the future study of molecular mechanism regulation of spermatogenesis.
Recycling construction and demolition waste not only reduces project costs; and saves natural resources, but also solves the environmental threat caused by construction waste disposal. In this paper, C25 waste road concrete is used as an experimental material, the uniaxial compression strength and tensile splitting strength of C25 RAC whose coarse aggregate replacement rate is 0%, 25%, 50%, 75%, and 100% are tested under the condition that the water-to-cement ratio is 0.47, 0.55 and 0.61. The results show: (1) the uniaxial compression strength and tensile splitting strength decrease with the increase of RAC; (2) for concrete with the same water-to-cement ratio, when the coarse aggregate replacement rate changes from 0% to 50%, the uniaxial compression strength and tensile splitting strength of RAC changes slightly. When the coarse aggregate replacement rate changes from 50% to 100%, the uniaxial compression strength and tensile splitting strength of RAC decreases rapidly
In this study, medium-carbon steel was subjected to warm deformation experiments on a Gleeble 3500 thermosimulator machine at temperatures of 550°C and 650°C and strain rates of 0.001 s–1 to 1 s–1. The warm deformation behavior of martensite and the effects of strain rate on the microstructure of ultrafine grained medium-carbon steel were investigated. The precipitation behavior of Fe3C during deformation was analyzed and the results showed that recrystallization occurred at a low strain rate. The average ultrafine ferrite grains of 500 ± 58 nm were fabricated at 550°C and a strain rate of 0.001 s–1. In addition, the size of Fe3C particles in the ferrite grains did not show any apparent change, while that of the Fe3C particles at the grain boundaries was mainly affected by the deformation temperature. The size of Fe3C particles increased with the increasing deformation temperature, while the strain rate had no significant effect on Fe3C particles. Moreover, the grain size of recrystallized ferrite decreased with an increase in the strain rate. The effects of the strain rate on the grain size of recrystallized ferrite depended on the deformation temperature and the strain rate had a prominent effect on the grain size at 550°C deformation temperature. Finally, the deformation resistance apparently decreased at 550°C and strain rate of 1 s–1 due to the maximum adiabatic heating in the material.
The effects of Mg and Ca on sulfide modification of sulphur steel were studied to elucidate the difference between micromagnesium treatment and micro-calcium treatment for the inclusion of sulphur steel. The results show that the inclusions in the steel appeared with an oxide core of Al2O3 and MnS wrapped. After the addition of Mg, the core was changed to spinel, and the MnS coating was changed to Mn-Mg-S. After Ca was added, the core was changed to Ca-Al-O, and the MnS coating was changed to Mn-Ca-S. The Mg content was higher than Ca content in the sulfides of the steel. Therefore, Mg was more effective than Ca in terms of sulfide modification with the same content of Mg and Ca in steel, but the yielding rate of Mg was lower than that of Ca. The Mg content in the oxide core was higher than Mg of the coating of the inclusions in the steel treated with Mg or Mg-Ca. In contrast, the Ca content in the oxide core was lower than Ca of the coating of the inclusions in the steel treated with Ca or Mg-Ca. MnS formed and precipitated during the melt solidification process. The complex sulfide (Mg-Mn-S) was precipitated around MgO·Al2O3 in the Mg treated steel during the cooling process. CaS inclusion was precipitated on the CaO·Al2O3 inclusions in the liquid Ca-treated steel. Thus, CaS was formed first, whereas MnS was formed during the cooling process, followed by the formation of complex sulfide (CaS+MnS), which finally precipitated around CaO·Al2O3 in the Ca-treated steel.