Duck viral hepatitis (DVH) is an acute and fatal disease of young ducklings characterized by rapid transmission and damages. The most important agent of DVH is duck hepatitis virus 1 (DHV-1). The effective control of DVH was achieved by active immunization of 1-day-old duck- lings with an attenuated DHV-1 virus vaccine. However, the attenuated virus might reverse to virulence. In this study, a DHV-1 strain, Du/CH/LBJ/090809, was identified and its genomic se- quences were determined. The genome of Du/CH/LBJ/090809 is composed of 7,692 nt excluding poly A and the virus was clustered into genotype A by comparing with other referenced DHV-1 strains. Du/CH/LBJ/090809 could lead to 30% mortality of 10-day-old specific pathogen free (SPF) ducklings. The virus was passaged serially in SPF chicken embryonated eggs and three vi- ruses, passage 16 (P16), P29 and P40, were selected for genomic analysis. P29 and P40 were used to evaluate the attenuation in duckling by inoculating the virus to 10-day-old SPF ducklings. Re- sults of vaccination-challenge assay showed that the inactivated virus P40 could evoke protection against the pathogenic parent virus. Nucleotide and amino acid sequences of the genomes of Du/ CH/LBJ/090809, P16, P29 and P40 were compared. Changes both in nucleotides and amino acids, which might be contributed to the decreasing in virulence by chicken embryo-passaging of DHV- 1, were observed. We speculated that these changes might be important in the adaption and at- tenuation of the virulent virus. Additionally, strains obtained in this study will provide potential candidate in the development of vaccines against DHV-1.
The pathological states of biological tissue are often resulted in attenuation changes. Thus, information about attenuating properties of tissue is valuable for the physician and could be useful in ultrasonic diagnosis. We are currently developing a technique for parametric imaging of attenuation and we intend to apply it for in vivo characterization of tissue. The attenuation estimation method based on the echoes mean frequency changes due to tissue attenuation dispersion, is presented. The Doppler IQ technique was adopted to estimate the mean frequency directly from the raw RF data. The Singular Spectrum Analysis technique was used for the extraction of mean frequency trends. These trends were converted into attenuation distribution and finally the parametric images were computed. In order to reduce variation of attenuation estimates the spatial compounding method was applied. Operation and accuracy of attenuation extracting procedure was verified by calculating the attenuation coefficient distribution using the data from the tissue phantom (DFS, Denmark) with uniform echogenicity while attenuation coefficient underwent variation.
The attenuating properties of biological tissue are of great importance in ultrasonic medical imaging. Investigations performed in vitro and in vivo showed the correlation between pathological changes in the tissue and variation of the attenuation coefficient. In order to estimate the attenuation we have used the downshift of mean frequency (fm) of the interrogating ultrasonic pulse propagating in the medium. To determine the fm along the propagation path we have applied the fm estimator (I/Q algorithm adopted from the Doppler mean frequency estimation technique). The mean-frequency shift trend was calculated using Single Spectrum Analysis. Next, the trends were converted into attenuation coefficient distributions and finally the parametric images were computed. The RF data were collected in simulations and experiments applying the synthetic aperture (SA) transmit-receiving scheme. In measurements the ultrasonic scanner enabling a full control of the transmission and reception was used. The resolution and accuracy of the method was verified using tissue mimicking phantom with uniform echogenicity but varying attenuation coefficient.
Noise measurements have been carried out at eleven different sites located in three prominent cities of the Tarai region of India to evaluate the effectiveness of vegetation belts in reducing traffic noise along the roadsides. Attenuation per doubling of distance has been computed for each site and excess attenuation at different 1/3 octave frequencies has been estimated. The average excess attenuation is found to be approximately 15 dB over the low frequencies (200 Hz to 500 Hz) and between 15 dB to 20 dB over the high frequencies (8 kHz to 12.5 kHz). Over the critical middle frequencies (1-4 kHz), the average excess attenuation (between 10-15 dB) though not as high, is still significant, with a number of sites showing an excess attenuation of 15 dB or more at 1 kHz. The results indicate that sufficiently dense vegetation belts along the roadsides may prove as effective noise barriers and significant attenuation may be achieved over the critical middle frequencies (1-4 kHz).
Porous materials are used in many vibro-acoustic applications. Different models describe their perfor- mance according to material’s intrinsic characteristics. In this paper, an evaluation of the effect of the porous and geometrical parameters of a liner on the acoustic power attenuation of an axisymmetric lined duct was performed using multimodal scattering matrix. The studied liner is composed by a porous ma- terial covered by a perforated plate. Empirical and phenomenal models are used to calculate the acoustic impedance of the studied liner. The later is used as an input to evaluate the duct attenuation. By varying the values of each parameter, its influence is observed, discussed and deduced
The impulse noise is agent harmful to health not only in the case of shots from firearms and the explosions of explosive materials. This kind of noise is also present in many workplaces in the industry. The paper presents the results of noise parameters measurements in workplaces where four different die forging hammers were used. The measured values of the C-weighted peak sound pressure level, the A-weighted maximum sound pressure level and A-weighted noise exposure level normalized to an 8 h working day (daily noise exposure level) exceeded the exposure limit values. For example, the highest measured value of the C-weighted peak sound pressure level was 148.9 dB. In this study possibility of the protection of hearing with the use of earplugs or earmuffs was assessed. The measurement method for the measurements of noise parameters under hearing protection devices using an acoustical test fixture instead of testing with the participation of subjects was used. The results of these measurements allows for assessment which of two tested earplugs and two tested earmuffs sufficiently protect hearing of workers in workplaces where forging hammers are used.
This article presents an efficient method of modelling acoustic phenomena for real-time applications such as computer games. Simplified models of reflections, transmission, and medium attenuation are described along with assessments conducted by a professional sound designer. The article introduces representation of sound phenomena using digital filters for further digital audio processing.
Internal casting defects that are detected by radiography may also be detected by ultrasonic method. Ultrasonic testing allows investigation of the cross-sectional area of a casting, it is considered to be a volumetric inspection method. The high frequency acoustic energy travels through the casting until it hits the opposite surface or an interface or defect. The interface or defect reflects portions of the energy, which are collected in a receiving unit and displayed for the analyst to view. The pattern of the energy deflection can indicate internal defect. Ultrasonic casting testing is very complicated in practice. The complications are mainly due to the coarse-grain structure of the casting that causes a high ultrasound attenuation. High attenuation then makes it impossible to test the entire volume of material. This article is focused on measurement of attenuation, the effect of probe frequency on attenuation and testing results.
This paper considers the assessment of attenuation in aluminium alloys castings and in cast iron prepared by gravity casting method and by casting under pressure. The issue of ultrasound attenuation is important in setting the conditions of non-destructive (NDT) testing, especially in casted materials. The characteristics of the ultrasonic technique and ultrasonic attenuation and the calculation of the attenuation and the velocity of ultrasound are presented in the theoretical part of this paper. For experimental measurements, cylindrical castings from AlSi alloy (a hypoeutectic alloy with a silicon content of about 7% - AlSi7 and a eutectic alloy with a silicon content of about 12% - AlSi12) and from grey and ductile cast iron were made. The ultrasonic records of the casting control, the calculation of ultrasound attenuation for individual samples are listed and described in the experimental part. The evaluation of measurements and comparison of calculated ultrasound attenuation is at the end of this article.
The paper is devoted to grain-refinement of the medium-aluminium zinc based alloys (MAl-Zn). The system examined was sand cast Zn10 wt. %. Al binary alloy (Zn-10Al) doped with commercial Al-3 wt. % Ti – 0.15 wt. % C grain refiner (Al-3Ti-0.15C GR). Basing on the measured attenuation coefficient of ultrasonic wave it was stated that together with significantly increased structure fineness damping decreases only by about 10 – 20%. The following examinations should establish the influence of the mentioned grain-refinement on strength and ductility of MAl-Zn cast alloys.
The void fraction is one of the most important parameters characterizing a multiphase flow. The prediction of the performance of any system operating with more than single phase relies on our knowledge and ability to measure the void fraction. In this work, a validated simulation study was performed in order to predict the void fraction independent of the flow pattern in gas-liquid two-phase flows using a gamma ray 60Co source and just one scintillation detector with the help of an artificial neural network (ANN) model of radial basis function (RBF). Three used inputs of ANN include a registered count under Compton continuum and counts under full energy peaks of 1173 and 1333 keV. The output is a void fraction percentage. Applying this methodology, the percentage of void fraction independent of the flow pattern of a gas-liquid two-phase flow was estimated with a mean relative error less than 1.17%. Although the error obtained in this study is almost close to those obtained in other similar works, only one detector was used, while in the previous studies at least two detectors were employed. Advantages of using fewer detectors are: cost reduction and system simplification.
It is meaningful to study the issues of CO migration and its concentration distribution in a blind gallery to provide a basis for CO monitoring and calculation of fume-drainage time, which is of a great significance to prevent fume-poisoning accidents and improve efficiency of an excavation cycle. Based on a theoretical analysis of a differential change of CO mass concentration and the CO dispersion model in a fixed site, this paper presents several blasting fume monitoring test experiments, carried out with the test location to the head LP in arrange of 40-140 m. Studies have been done by arranging multiple sensors in the arch cross-section of the blind gallery, located at the Guilaizhuang Gold Mine, Shandong Province, China. The findings indicate that CO concentrations in the axial directions are quadratic functions with the Y and Z coordinate values of the cross-section of the blind gallery in an ascending stage of CO time- -concentration curve, with the maximum CO concentrations in Y = 150 cm and Z = 150 cm. Also, the gradients of CO concentration in the gallery are symmetrical with the Y = 150 cm and Z = 150 cm. In the descending stage of CO time-concentration curve, gradients of CO concentration decrease in lateral sides and increase in the middle, then gradually decrease at last. The rules of CO concentration distribution in the cross-section are that airflow triggers the turbulent change of the CO distribution volume concentration and make the CO volume concentration even gradually in the fixed position of the gallery. Moreover, the CO volume concentrations decrease gradually, as well as volume concentration gradients in the cross-section. The uniformity coefficients of CO concentration with duct airflow velocities of 12.5 m/s, 17.7 m/s and 23.2 m/s reach near 0.9 at 100-140 m from the heading to the monitoring spot. The theoretical model of a one-dimensional migration law of CO basically coincides with the negative exponential decay, which is verified via fitting. The average effective turbulent diffusion coefficient of CO in the blind gallery is approximate to 0.108 m2/s. There are strong linear relationships between CO initial concentration, CO peak concentrations and mass of explosive agent, which indicates that the CO initial concentration and the CO peak concentration can be predicted, based on the given range of the charging mass. The above findings can provide reliable references to the selection, installation of CO sensors and prediction of the fume-drainage time after blasting.
Acoustic properties of ultrasound (US) contrast agent microbubbles (MB) highly influence sonoporation efficiency and intracellular drug and gene delivery. In this study we propose an acoustic method to monitor passive and excited MBs in a real time. MB monitoring system consisted of two separate transducers. The first transducer delivered over an interval of 1 s US pulses (1 MHz, 1% duty cycle, 100 Hz repetition frequency) with stepwise increased peak negative pressure (PNP), while the second one continuously monitored acoustic response of SonoVue MBs. Pulse echo signals were processed according to the substitution method to calculate attenuation coefficient spectra and loss of amplitude. During US exposure at 50–100 kPa PNP we observed a temporal increase in loss of amplitude which coincided with the US delivery. Transient increase in loss of amplitude vanished at higher PNP values. At higher PNP values loss of amplitude decreased during the US exposure indicating MB sonodestruction. Analysis of transient attenuation spectra revealed that attenuation coefficient was maximal at 1.5 MHz frequency which is consistent with resonance frequency of SonoVue MB. The method allows evaluation of the of resonance frequency of MB, onset and kinetics of MB sonodestruction.
The soft tissue attenuation is an interesting parameter from medical point of view, because the value of attenuation coefficient is often related to the state of the tissue. Thus, the imaging of the attenuation coefficient distribution within the tissue could be a useful tool for ultrasonic medical diagnosis. The method of attenuation estimation based on tracking of the mean frequency changes in a backscattered signal is presented in this paper. The attenuation estimates are characterized by high variance due to stochastic character of the backscattered ultrasonic signal and some special methods must be added to data processing to improve the resulting images. The following paper presents the application of Spatial Compounding (SC), Frequency Compounding (FC) and the combination of both. The resulting parametric images are compared by means of root-mean-square errors. The results show that combined SC and FC techniques significantly improve the quality and accuracy of parametric images of attenuation distribution.
The article deals with ultrasonic testing possibilities of the copper alloy centrifugal casts. It focused on the problems that arise when testing of castings is made of non-ferrous materials. Most common types of casting defects is dedicated in theoretical introduction of article. Ultrasonic testing technique by conventional ultrasound system is described in the theoretical part too. Practical ultrasonic testing of centrifugal copper alloy cast - brass is in experimental part. The experimental sample was part of centrifugally cast brass ring with dimensions of Ø1200x34 mm. The influence of microstructure on ultrasonic attenuation and limitations in testing due to attenuation is describes in experimental part. Conventional direct single element contact ultrasound probe with frequencies of 5 MHz, 3.5 MHz and 2 MHz were used for all experimental measurements. The results of experimental part of article are recommendations for selecting equipment and accessories for casting testing made of non-ferrous metals.
The paper presents results of measuring attenuation coefficient of the Al-20 wt.% Zn alloy (AlZn20) inoculated with different grain refiners. During experiments the melted alloys were doped with Al-Ti3-C0.15 refining master alloy. Basing on measurements performed by Krautkramer USLT2000 device with 1MHz ultrasound wave frequency it was stated that grain refinement reduces the attenuation coefficient by about 20-25%. However, the examined alloys can be still classified as the high-damping ones of attenuation greater than 150 dB/m.
In this work we present the design and the manufacturing processes, as well as the acoustics standardization tests, of an acoustic barrier formed by a set of multi-phenomena cylindrical scatterers. Periodic arrangements of acoustic scatterers embedded in a fluid medium with different physical properties are usually called Sonic Crystals. The multiple scattering of waves inside these structures leads to attenuation bands related to the periodicity of the structure by means of Bragg scattering. In order to design the acoustic barrier, two strategies have been used: First, the arrangement of scatterers is based on fractal geometries to maximize the Bragg scattering; second, multi-phenomena scatterers with several noise control mechanisms, as resonances or absorption, are designed and used to construct the periodic array. The acoustic barrier reported in this work provides a high technological solution in the field of noise control.
The linear 3D piezoelasticity theory along with active damping control (ADC) strategy are applied for non-stationary vibroacoustic response suppression of a doubly fluid-loaded functionally graded piezolaminated (FGPM) composite hollow cylinder of infinite length under general time-varying excitations. The control gain parameters are identified and tuned using Genetic Algorithm (GA) with a multi-objective performance index that constrains the key elasto-acoustic system parameters and control voltage. The uncontrolled and controlled time response histories due to a pair of equal and opposite impulsive external point loads are calculated by means of Durbin’s numerical inverse Laplace transform algorithm. Numerical simulations demonstrate the superior (good) performance of the GA-optimized distributed active damping control system in effective attenuation of sound pressure transients radiated into the internal (external) acoustic space for two basic control configurations. Also, some interesting features of the transient fluid-structure interaction control problem are illustrated via proper 2D time domain images and animations of the 3D sound field. Limiting cases are considered and accuracy of the formulation is established with the aid of a commercial finite element package as well as comparisons with the current literature.