This paper recapitulates Polish botanical and mycological research on terrestrial and freshwater Antarctic ecosystems carried out between 1977 and 2009. The main results are briefly summarized. The references encompass nearly 200 papers on floristics, taxonomy, biogeography, ecology, cytology, bioc hemistry, physiology and genetics of lichens, mosses, fungi, algae and vascular plants inhabiting soils, rocks and inland waters in the Antarctic.
This study was executed to investigate the potential of agar-agar, a nontoxic and non-degradable gelling agent, as a promising coating agent to improve and protect banana fruit against fungal postharvest diseases i.e., crown, finger, neck and flower end rots which are caused by fungal isolates of Colletotrichum musae and Fusarium moniliforme. Coated-ba-nana fruit samples with different concentrations of agar-agar suspension particularly at 2.0 g · l−1 exhibited a significant reduction in incidence and severity of postharvest diseases compared to untreated fruit. Banana fruits dipped in agar suspension at 2.0 g · l−1 for 5, 10 and 15 min showed significant reduction in disease incidence and severity. Moreover, application of agar suspension as a coating agent at 2.0 g · l−1 significantly decreased weight loss (%), firmness loss (%), and soluble solid concentration of banana fruit for 15 days at 25 ± 2°C. Scanning electron microscopy observation confirmed that the fruit coated with agar colloid at 2.0 g · l−1 had significantly fewer cracks and showed smoother surfaces than untreated fruit. This explains the quality improvement in agar-coated fruit compared to uncoated fruit. Overall, agar colloid, a safe coating agent, could be used to protect banana fruit against postharvest rot diseases and extend fruit storage life during ripening and storage.
The present contribution to lichen−forming and lichenicolous biota of northern− most Billefjorden (Petuniabukta area, central Spitsbergen, Svalbard) contains 40 species of lichens. Four species: Arthonia ligniariella, Candelariella lutella, Ochrolechia upsaliensis, Polyblastia pernigrata are new for the Svalbard Archipelago.
Lichens of relict penguin colonies and sites affected by active penguin colonies were investigated in Victoria Land, Ross Sea sector, continental Antarctica. A total of 17 coastal sites, seven in northern and ten in southern Victoria Land, have been investigated across 7 ° of latitude from 71 ° to 78 ° S. Altogether 40 taxa of lichens have been identified. Four of the recorded species are new to the Antarctic – Caloplaca erecta , C. soropelta , C. tominii and Physcia tenella ; two species are new to the Victoria Land area – Lecania nylanderiana and Lecanora polytropa . The first lichen records from Beaufort Island are also provided. Data presented here expand the knowledge on the occurrence, diversity and distribution of Victoria Land lichens.
The species structure of plant parasitic nematode populations from the rhizosphere of winter wheat grown with crop rotation or in 48-year-old monoculture was analyzed and compared. Dominating species: Bitylenchus dubius, Merlinius microdorus, Paratylenchus neglectus and Heterodera avenae, in monoculture plots, had higher populations than in crop rotation plots. Heterodera avenae eggs and larvae were infected by pathogenic fungi in 68% of the monoculture crops (vs. 65–66% of the cysts from crop rotation), 12–20% of Paratylenchus sp. specimens were colonized by bacteria, mainly by Bacillus penetrans. This study shows nematological changes occurring in long-term wheat breeding, thus providing additional information necessary to fight dangerous viral vectors of the examined cereal.
This paper refers to lichen biota growing on driftwood in the Kaffi ø yra Plain (NW Spitsbergen, Svalbard). The presented list of 25 lichenized fungi includes both the eurytopic, accidental, typical, and stenotopic species. Taxa that belong to the last two groups can be considered as lignicolous. This study confirms the existence of a specific group of lichen species, for which the driftwood is a main substrate in the Arctic. Additionally, five lichen species new for the whole Svalbard were recorded, namely: Candelariella coralliza , Elixia flexella , Lecanora saligna , Lecidea plebeja , and Xylographa sibirica .
The aim of this study was to identify a suitable lichen species for the long−term monitoring of heavy−metal atmospheric pollution in Svalbard. Cladonia and Cetraria s.l. species that have been widely used until now for assessing heavy−metal deposition in the Arctic are in decline over extensive areas of Svalbard, mainly due to climate change and over−grazing by reindeer. Cetrariella delisei , rarely used for biomonitoring, is still common and widespread in this area. Levels of Cr, Ni, Fe, Cu, Pb, Zn, Cd and Mn were measured in three lichen species: Cetrariella delisei , Cladonia uncialis , Flavocetraria nivalis and in a moss Racomitrium lanuginosum from Sørkapp Land, South Spitsbergen. The results imply that Cetrariella delisei can be safely compared to Cladonia uncialis for identifying the levels of heavy metals, but direct comparison between Cetrariella delisei and other species studied is more difficult owing to differences in levels of heavy metals even in samples from the same site.
Many species of Trichoderma produce secondary metabolites such as volatile organic compounds (VOCs) that reduce plant diseases and promote their growth. In this work we evaluated the antagonistic effects of VOCs released by eight strains of two Trichoderma species against Pyrenophora teres Drechsler, the causal agent of barley net blotch. Antagonism was estimated based on the percentage of mycelial growth inhibition according to the confronted cultures method. VOCs extraction and identification were performed by gas chromatography and mass spectrometry, through different methodologies for VOCs emitted by antagonists and pathogens alone or when confronted. VOCs produced by all Trichoderma strains inhibited mycelial growth of the pathogen in a range of 3 to 32%, showing weak and unpigmented mycelia with vacuolization. In addition, P. teres stimulated the release of VOCs by both Trichoderma species. The major groups of VOCs detected were sesquiterpenes, followed by diterpenes, terpenoids and eight-carbon compounds. This is the first report about characterization of volatiles emitted by Trichoderma in the presence of P. teres.
Arbuscular mycorrizal (AM) fungi may enhance plant growth and polyphenol production, however, there have been limited studies on the relationships between root colonization of different fungal species and polyphenol production on cultivated Allium porrum (garden leek). The effects of inoculation of AM fungi spores from Rhizophagus intraradices, Giga -spora margarita, Glomus geosporum, Paraglomus occultum, Claroideoglomus claroideum, and Glomus species on colonization of garden leek roots and symbiotic changes in polyphenol production and plant growth were evaluated in greenhouse experiments. There were significant differences (p < 0.05) in colonization of leek roots by AM fungi species. The greatest level of root colonization was recorded on plants inoculated with R. intraradices (73%) and the lowest level on C. claroideum (3.2%). Significant differences (p < 0.05) in plant height were recorded between AM inoculated plants and the controls. Polyphenol levels differed significantly (p < 0.05) between garden leek plants inoculated with AM fungi and the non-inoculated controls. The percentage increases in polyphenol (a derivative of kaempferol) on garden leeks inoculated with G. geosporum relative to the untreated controls ranged from 28 to 1123%. Due to symbiosis with different AM species, other polyphenols decreased in some instances (negative values) and increased in others for values of up to 590%. Results showed that AM fungi species exhibited remarkable differences in polyphenol levels in garden leeks. The high polyphenol production by garden leek plants inoculated with G. geosporum, and Glomus species could be exploited for enhanced resistance of garden leeks to insects and diseases. This research highlights an understudied area, notably the relationships between AM fungal inoculations, root colonizations and polyphenol production in garden leeks. The findings can be utilized to improve pest resistance and the quality of garden leek plants.
Two fungal strains, isolated from Livingston Island, Antarctica (Penicillium commune 161, psychrotolerant and Aspergillus glaucus 363, mesophilic) were investigated for a relationship between growth temperature and oxidative stress response. Cultivation at temperatures below - (10 and 15°C and 10 and 20°C for P. commune and A. glaucus, respectively) and above (25°C and 30°C for P. commune and A. glaucus, respectively) the optimum caused significant difference in growth and glucose uptake in comparison with the control cultures. Enhanced level of reserve carbohydrates (glycogen and trehalose) was determined under cultivation at different temperatures from the optimal one. While the highest content of trehalose was found in the exponential phase, glycogen accumulation was observed in the stationary phase when growth conditions deteriorate. The growth at temperature below- and above-optimum caused strain-dependent changes in two antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT). While SOD activity in the psychrotolerant strain increases with decreasing of growth temperature, the mesophilic A. glaucus demonstrated marked reduction of it at below- and above-optimal temperature. Decreasing trend of CAT activity was observed in both strains below the optimal temperature indicating a lack of antioxidant protection from this enzyme under the cold stress conditions.