The development of in vitro embryo production (IVEP) techniques in Felis catus is a fitting model with potential application to the conservation of endangered felid species. To improve the quality of IVEP techniques an appropriate balance of pro- and antioxidants should be provided. Under in vitro conditions, high levels of superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) mRNA provide a defence mechanism against oxidative stress for embryos. In order to improve the development of cat oocytes, the effects of SOD and CAT supplemented to in vitro maturation (IVM) medium and of GPx supplemented to in vitro fertilization (IVF) medium on development and embryo production in vitro were evaluated. Data showed an increase of 70 and 77 % of cleaved embryo and blastocyst formation, respectively, in the experiment with SOD and CAT addition to IVM medium; in the experiment with GPx addition to IVF medium the number of cleaved embryos doubled and the number of embryos increased by 96 %. Therefore, our results were positive and encourage us to continue studies on cat oocytes evaluating the effects of various dosages and combination of antioxidants.
Oxidative stress (OxS) has been implicated in the pathogenesis of Crohn’s disease (CD). The aim of this study was to examine whether nonenzymatic antioxidants are associated with active CD, by using the FRAP and GSH assay in plasma. Additionally, we measured bilirubin and albumin levels as two individual components of the plasma antioxidant system. A total of 55 patients with established CD, 30 with active CD and 25 with inactive disease, and 25 healthy individuals were prospectively enrolled in this study. We evaluated CD activity index, BMI and blood morphology, platelet count, serum CRP level, and bochemical parameters of OxS: ferric reducing ability of plasma (FRAP), reduced glutathione (GSH) in plasma and bilirubin and albumin levels in serum. Plasma FRAP and GSH concentrations were decreased in both CD groups compared to controls and negatively correlated with CDAI values (FRAP: r = –0.572, p = 0.003; GSH: r = –0.761, p = 0.001), CRP and platelet count. Bilirubin and albumin levels were lower in the serum of active CD patients than inactive CD patients and controls and negatively correlated with the CD activity index (r = –0328, p = 0.036, r = –0.518, p = 0.002) and CRP (r = –0.433, p = 0.002). The decreased FRAP and GSH levels in plasma and bilirubin and albumin levels in serum of patients with active CD compared to inactive CD and controls underlines the importance of OxS in the pathophysiology and activity of CD.
Nanotechnology has been widely applied in agriculture, and understanding of the mechanisms of plant interaction with nanoparticles (NPs) as environmental contaminants is important. The aim of this study was to determine the effects of foliar application of cobalt oxide (Co3O4) NPs on some morpho-physiological and biochemical changes of canola (Brassica napus L.) leaves. Seeds were sown in plastic pots and grown under controlled conditions. Fourteen-day-old seedlings were sprayed with different concentrations of Co3O4 NPs (0, 50, 100, 250, 500, 1000, 2000, and 4000 mg L-1) at weekly intervals for 5 weeks. Growth parameters of the shoot (length, fresh and dry weights) were stimulated by low concentrations of Co3O4 NPs (50 and 100 mg L-1) and repressed by higher concentrations. Similar trends were observed in photosynthetic pigment contents. The results indicated that high concentrations of Co3O4 NPs increased lipoxygenase (LOX) activity and the malondialdehyde (MDA), hydrogen peroxide (H2O2), and dehydroascorbate (DHA) contents, but reduced the membrane stability index (MSI), ascorbate (ASC), and glutathione (GSH). Despite the increase of antioxidant capacity (DPPH) and the accumulation of nonenzymatic antioxidants (total flavonoids and flavonols) and osmolytes (proline, glycine betaine (GB) and soluble sugars) at high concentrations of Co3O4 NPs, the growth and photosynthesis were reduced. The defence system activity did not seem to be sufficient to detoxify reactive oxygen species (ROS). Altogether, high concentrations of Co3O4 NPs showed a phytotoxic potential for canola as an oilseed crop.