The radial distribution system is a rugged system, it is also the most commonly used system, which suffers by loss and low voltage at the end bus. This loss can be reduced by the use of a capacitor in the system, which injects reactive current and also improves the voltage magnitude in the buses. The real power loss in the distribution line is the I2R loss which depends on the current and resistance. The connection of the capacitor in the bus reduces the reactive current and losses. The loss reduction is equal to the increase in generation, necessary for the electric power provided by firms. For consumers, the quality of power supply depends on the voltage magnitude level, which is also considered and hence the objective of the problem becomes the multi objective of loss minimization and the minimization of voltage deviation. In this paper, the optimal location and size of the capacitor is found using a new computational intelligent algorithm called Flower Pollination Algorithm (FPA). To calculate the power flow and losses in the system, novel data structure load flow is introduced. In this, each bus is considered as a node with bus associated data. Links between the nodes are distribution lines and their own resistance and reactance. To validate the developed FPA solutions standard test cases, IEEE 33 and IEEE 69 radial distribution systems are considered.
To keep genetic diversity, flowering plants have developed a self-incompatibility system, which can prevent self-pollination. It has been reported that calcium concentration in pistil papilla cells was increased after self-pollination in transformed self-incompatible Arabidopsis thaliana. In this study, we found that CML27 changed its expression level for both mRNA and protein when compared to transcriptome and proteome. At the same time, CML27 was expressed in the anther and pistil at a high level and reached up to 5-fold up-regulated expression in the pistil at 1 h post-pollination when compared to 0 min. In order to find out potential proteins that may interact with BoCML27, BoCML27 was expressed in and isolated from E. coli. After its co-incubation with Brassica oleracea pistil proteins, the products were separated on SDS-PAGE gels. We found a specific band at the position between 130–180 kDa. Through LC-MS-MS (Q-TOF) analysis, eight proteins were identified from the band. The proteins include 26S proteasome non-ATPase regulatory (26S), Phospholipase D, alpha 2 (PLDα2) involved in Ca2+ binding and Coatomer subunit alpha-2-like (Coatomer) involved in vesicle mediated transport. All of these identified proteins provide new insights for the self-incompatibility response in B. oleracea, specific for increasing Ca2+ concentration in pistil papilla cells.