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Abstract

Helicobacter species have been reported in animals, some of which are of zoonotic importance. This study aimed to detect Helicobacter species among human and animal samples using conventional PCR assays and to identify their zoonotic potentials. Helicobacter species was identified in human and animal samples by genus-specific PCR assays and phylogenetic analysis of partial sequencing of the 16S ribosomal RNA gene. The results revealed that Helicobacter species DNA was detected in 13 of 29 (44.83%) of the human samples. H. pylori was identified in 2 (15.38%), and H. bovis was detected in 4 (30.77%), whereas 7 (53.85%) were unidentified. H. bovis and H. heilmannii were prevalent among the animal samples. Phylogenetic analysis revealed bootstrapping of sequences with H. cinaedi in camel, H. rappini in sheep and humans, and Wollinella succinogenes in humans. In conclusion, the occurrence of non-H. pylori infections among human and animal samples suggested zoonotic potentials.
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Authors and Affiliations

A.I. Youssef
1
A. Afifi
2
S. Abbadi
3
A. Hamed
4
M. Enany
2

  1. Animal Hygiene and Zoonoses, Faculty of Veterinary Medicine, Suez Canal University, 41522, 4.5 Km Ring Road, Ismailia, Egypt
  2. Microbiology and Immunology Department, Faculty of Veterinary Medicine, Suez Canal University, Egypt
  3. Microbiology and Immunology Department, Faculty of Medicine, Suez University, 43512, Alsalam City, Suez, Egypt
  4. Biotechnology Department, Animal Health Research Institute, P.O. Box 264, Dokki, Giza 12618, Egypt

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