High voltage DC insulation plays an important role, especially in power transmission systems (HVDC) but also increasingly on medium voltage levels (MVDC). The space charge behavior under DC voltage has great importance on electrical insulation reliability. This paper reports investigations of encapsulated space charge in homo-multilayer dielectric materials using the pulsed electro-acoustic (PEA) method. The charge has been introduced on the homo-layer interface by corona sprinkling prior to encapsulation. Two doses of charge density were accumulated on the dielectric surface in two types of dielectric materials Kapton and LDPE. The polarization DC voltage was applied in 2 min intervals in steps corresponding to an effective electric field strength in a range of 8-40 kV/mm for Kapton and 10-50 kV/mm for LDPE. The PEA-based detected space charge was compared at the initial, reference stage, prior to charge accumulation, and after corona sprinkling of defined charge density. The evaluation was based on the PEA time-dependent charge distributions and charge profiles referring to the DC polarization field strength. The goal of the experiment was to identify the relationship and the character of the known sprinkled and encapsulated charge inside homo-layered materials using the PEA method. According to the observations, the ratio between sprinkled charge densities is proportional to the encapsulated, charge densities measured by the PEA method on the interfacial homo-layer for the Kapton specimen. In the case of LDPE, a fast decrease of interfacial charge was observed, especially at a higher polarization field above 10 kV/mm. The encapsulation of the known charge amount can be extended to different types of multilayer material. The presented methodology might be used also for extended calibration of the PEA measurement system.