The purpose of this study has been to determine the effect of fertilisation with urea-ammonium nitrate (UAN) solution enriched with P, Mg or S on the content of macronutrients in the grain and straw of maize. The following fertilisers were tested in the field experiment: ammonium nitrate, urea, UAN – 32% N; RSM+S – 26% N + 3% S; RSM+P(Medium) – 26% N and 4.80% P; RSM+P(Starter) – 21% N and 7.86% P; UAN + Mg – 20% N + 4% Mg. In each year of the experiment, significant differentiation in the contents of P, K, Ca, Mg and S in maize grain and straw was observed, depending on the applied nitrogen fertilisation. However, considering the average values from each treatment achieved over the three years, it was demonstrated that the fertilisation significantly changed only the content of P and S in grain and K and Ca in straw of maize. The removal of nutrients was the highest in the second year of the research and amounted in kg∙ha ^–1: P – about 100, K – about 350, Ca – about 80, Mg – about 35 and S – about 31, which in turn were differentiated over the years of the experiment in the three years. The removal of P, K, Mg and S also significantly depended on fertilisation. Significant differences, however, most often concerned the control treatment relative to the fertilised ones. The contribution of grain to the accumulation of nutrients also varied significantly in the three years of the experiment. Significantly the lowest share of grain in terms of P and S accumulation was noted in maize grown without N fertilisation.