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Number of results: 4
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Abstract

Hydrobionts are considered as highly potential source for bioproduction (including energy carriers and fertilizers) and many biotechnological processes that include hydrobionts, particularly their biomass as a substrate are used in different fields of energy, cosmetology, medicine, pharmaceutics, aquaculture, agriculture, forestry etc. Latest developments prove efficiency in applying anaerobic digestion for purifying wastewaters from organic pollutants with the help of macrophytes and microphytes in conducting biomethanogenesis. Many studies have established that it is possible to reach high level of lipid extraction from algae (to 95%) with the help of organic solvents (methanol, acetone, hexane, diethyl ether etc). Blue – green algae biomass has been scientifically proved to be a good source for methane, methanol, ethanol, propanol, isopropanol, biodiesel and other biofuel types production. Macroalgae and microalgae contain β- carotene, biotin, folic acid, fucoidans, lectins, phenolics, sulphated polysaccharides and other derived biologically active compounds that can be used in producing vitamins, have anti-ulcer, antioxidant, antibiotic, antifouling, immune modulatory and other properties. Cyanidioschyzon merolae, Ostreococcus lucimarinus, O. tauri, Micromonas pusilla have shown high potential for hydrogen production while Rhizoclonium sp. has been experimentally used as a bounding material in briquetting miscanthus granules, resulting in 20 % higher dynamic strength. The article is a literature review and the purpose of this work is to classify and systemize hydrobionts, reveal regularity of their growth, conduct critical analysis on existing biotechnologies on using separate representatives of aquatic biomes as a raw material and also to review ways of intensification for these biotechnologies.

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Authors and Affiliations

Christina Soloviy
ORCID: ORCID
Myroslav Malovanyy
ORCID: ORCID
Volodymyr Nykyforov
Serhiy Dihtyar
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Abstract

Osteocalcin is a major non-collagenous component of the bone extracellular matrix and is considered to be an indicative factor of osteoblast differentiation. In the present study, we detected osteocalcin expression in different antler areas and growth phases by immunohisto- chemistry. Osteocalcin was highly expressed in all areas during the mineralization period and in mesenchymal cell and chondrocyte areas during the rapid growth period. The nucleotide sequence of the osteocalcin gene in sika deer antler was determined. The open reading frame was 303 bp encoding a protein of 100 amino acids. The estimated molecular mass of osteocalcin was 10.38 kDa and the theoretical isoelectric point was 5.37. The osteocalcin gene with a 6× His-tag at the C-terminus was cloned into the pGEX-4T1 vector and expressed in Escherichia coli under optimal conditions. The recombinant soluble protein fused with GST was purified with Ni-NTA resin. The purified osteocalcin protein exhibited a significant increase in HA adhesion and promoted antler chondrocyte proliferation. Osteocalcin is an important factor in regulating the rapid growth and differentiation of deer antlers.

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Authors and Affiliations

X. Li
M. Liu
X. Bai
Y. Li
Y. Zhao
S. Wang
J. Wang
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Abstract

Luticola muticopsis is a characteristic species of polar and subpolar regions. Its morphological variability is not yet precisely described. In the investigated population the cells from capitate to shortened, flat rounded tips were observed. The range of dimensions of specimens was 8.8-40.6 μm x 5.5-17.6 μm, striae 11-22/10 μm; this range considerably exceeded that found in holotype diagnosis.

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Authors and Affiliations

Barbara Kawecka
Maria Olech
Maria Nowogrodzka-Zagórska

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