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Number of results: 5
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Abstract

Mathematical torrnulacs for rigorous adjustment of surveying modular networks are presented in the paper. The method is based on the idea of multigroup transformation. The solution leads to general problem of non-I i near adjusting of conditional equations with unknowns by the least square method. Il is a modification or the classical way of approximate adjustment of the modular networks.
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Authors and Affiliations

Tadeusz Gargula
ORCID: ORCID
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Abstract

Enterotoxigenic Escherichia coli (ETEC) is the causative agent of a wide range of diseases, which are the important cause of illness and mortality in piglets. ETEC strains expressing F4 fimbriae are frequently associated with post-weaning diarrhea (PWD) and lead to great economic losses in swine production industry worldwide. The aim of this study was to establish a rapid and effective isothermal amplification method for detection of F4 fimbriae. Loop-mediated isothermal amplification (LAMP), Polymerase spiral reaction (PSR) and cross-priming ampli- fication (CPA) were used to develop and optimize the detection method first time. Subsequently, the specificity and sensitivity of these methods were evaluated, and the clinical samples were detected with these methods. All the F4-positive samples could produce ladder-like amplifica- tions products and lead the chromogenic substrate SYBR Green I produce green fluorescence, while in blank control and negative samples lack of this pattern or remained orange. The sensi- tivity of LAMP and CPA were 10 times higher than PSR method. Meanwhile, these three methods were validated with clinical samples, 7 were found positive, while 125 samples were negative, the testing results were consisted with the real-time PCR method. These findings suggested that the isothermal amplification based on the F4 fimbriae is a rapid, effective and sensitive method under resource constrains.

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Authors and Affiliations

L.Y. Zhao
J.H. Niu
X.L. Gao
C.N. Liu
S.M. Liu
N. Jiang
X.P. Lv
S.M. Zheng
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Abstract

Regaining independence by each country (Tunisia, Morocco 1956, Algeria 1962) and the publication of relevant documents (codes of family law, constitutions) created opportunities to speak more widely about social and economic rights, or about political rights for women. However, the rights granted to women were characterized by the principle of inequality, especially in Algeria and Morocco. In this difficult and complex situation, the emancipation movement of women went through various phases. In Algeria, its strength began to appear at the turn of the seventies and eighties of the twentieth century and has been constantly increased. In Morocco, in principle, the awakening took place in the early nineties of the twentieth century. Women themselves played a significant role in the activities for emancipation, engaging in various undertakings, organizations and associations, and in activating Non Profit Organizations (Organisation Non-Gouvernementale -ONG) with women participation from the end of the 80s of the twentieth century, which, in its turn, created opportunities for legal reforms, which would not exist without activities carried out by various associations, including women’s associations. The Jasmine Revolution, also known as the Arabic spring, was initiated in Tunisia, and has had a significant impact on the contemporary activities of women.

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Authors and Affiliations

Anna Barska
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Abstract

Two species of Amphipoda (Hyperiidae), Themisto libellula (Mandt, 1822) and Themisto abyssorum (Boeck, 1870), were collected with the use of a WP-2 net from the area be­tween Nordkapp and Spitsbergen (73° to 78° N) in July of 1993,1996,1997 and 1998. Densities ranged from 6 to 992 ind. 100 m-3 (T. abyssorum) and from 8 to 448 ind. 100 m-3 (r. libellula) and respective total biomass of T. abyssorum from 65.6 to 81.2 mg d.w. 100 m-3 and T. libellula from 59.9 to 131.5 mg d.w. 100 m-3.

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Authors and Affiliations

Krzysztof Wencki
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Abstract

Studies on southern elephant seal (Mirounga leonina) were carried in Admiralty Bay (King George Island, South Shetland Islands) in summer 1978/1979. In the whole region of Bay the maximum number of 964 elephant seals was noticed on 5 Jan. 1979. The biggest nonbreeding colonies were observed in the mouth region of the Bay. In December the males made up 74% of the whole population, their number decreased to 17% in February. Most individuals belonged to the IV class of age (6—9 years old). Introductory observations of 24 h activity indicates four peaks of activity in the groups observed on the coast. The type of weather has influenced the activity of these groups and the frequency of descending to the sea.

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Authors and Affiliations

Wiesław Krzemiński

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