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Abstract

In the present work, bacteria of the Pseudomonas genus native to the Ecuadorian Amazon with the capacity to treat contaminated water and soils were selected. For this purpose, 20 soil samples from Amazon region with evidence of contamination were analysed. For identification, each sample was assigned a code according to the sampling area: Joya de los Sachas (S), Minga (M) and Siete de Julio-Shushufindi (SH). The cultures were performed in the combination of Bushnell Hass (BH) + Luria Bertani (LB) and Müeller-Hinton (MH) + Brucella agar (BA) media, all with the addition of diesel to verify their efficacy in the growth of bacteria capable of surviving in contaminated media. The combination with ideal results was that of BH + LB, by means of Gram-staining it was determined that 19 of the samples had interest microorganisms. To characterize the isolates at the species level, biochemical tests of: catalase, citrate, glucose, hemolytic activity and urease were applied, which allowed to confirm the existence of the Pseudomonas of interest. The results indicated that P. stutzeri (in samples S1 and M1), P. aeruginosa (in SH2 and SH5) and P. putida (in S7, S8, S10 and SH4) obtaining a total of 8 isolates (40%) of interest from the initial 19. With the results obtained from this work, an optimal culture method was standardized for the selection of bacteria with potential for treating contaminated soils and water.
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Authors and Affiliations

Henry Joel Escudero-López
1
ORCID: ORCID
Karla Anabel Serrano-Carrillo
2
ORCID: ORCID
Carlos Rodrigo Jácome-Pilco
1
ORCID: ORCID
Herminia del Rosario Sanaguano-Salguero
1
ORCID: ORCID
Isidro Favian Bayas-Morejón
1
ORCID: ORCID

  1. State University of Bolívar, Faculty of Agricultural Sciences, Agroindustry Career, Biotechnological Research and Development Center, Km 3 1/2 sector Alpachaca, CP: 020150, Guaranda, Ecuador
  2. San Pedro Educational Unit, Department of Education, Guaranda, Ecuador
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Abstract

Erbium-doped lead silicate glass has been investigated for near-infrared emission and up-conversion applications. Near-infrared emission due to 4I13/24I15/2 transition of Er3+ is relatively broad (70.5 nm) and long-lived (3.7 ms). Also, up-conversion luminescence spectra of Er3+ ions in lead silicate glass have been examined as a function of temperature. The relative intensities of luminescence bands corresponding to 2H11/24I15/2 and 4S3/24I15/2 transitions of Er3+ were determined with temperature. The fluorescence intensity ratio and temperature sensitivity were calculated. The maximum sensitivity for Er3+ doped lead silicate glass is close to 26.4 × 10−4 K−1 at T = 590 K.

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Authors and Affiliations

W.A. Pisarski
J. Pisarska
R. Lisiecki
W. Ryba-Romanowski

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