Immunoaffinity chromatography (IAC) is a fundamental isolation and purification tool which is incorporated in a substantial range of therapeutic and diagnostic applications. This study has reappraised the usefulness of immunoaffinity chromatography for the purification of polyclonal antibodies. Protein A based IAC is a convenient and reliable method for purification of IgG, from hyperimmunesera (HIS) raised in experimental animals such as rabbits, guinea pigs and mice to be utilized in pharmaceutics and diagnostics. The 146S fraction of Foot and Mouth Disease virus (FMDV) TCID50=10 ^5.6 was cultured on a baby hamster kidney cell line 21 (BHK-21), concentrated using salt precipitation method using PEG 6000, purified by size exclusion chromatography (SEC) using Sepharose-30 at 254nm absorbance. Purification of 146S FMDV was analyzed using 12% SDS-PAGE which provided two bands of light and heavy chains. The alum-based vaccine, consisting of ≥10μg of 146S FMDV, was applied in 10 male rabbits and 10 male guinea pigs and two animals of each group were taken as a negative control. The titer of serum was calculated using virus neutralization test. A Protein-A kit (Thermo scientific- 44667, 0528.2) was used to purify HIS raised against 146S FMDV and validated using 12% SDS PAGE in reducing condition. The data demonstrate that protein-A affinity chromatography is an efficient tool for the purification of antibodies from hyper-immune sera raised against 146S FMDV and can be used for the production of diagnostic kits e.g. Enzyme linked immuno-sorbent assay (ELISA) and radioimmunoassay.

The aim of the study was to analyse mechanical properties and microstructure of joints obtained using friction stir welding (FSW) technology. The focus of the study was on overlap linear FSW joints made of 1.4541 DIN 17441 steel sheets with thickness of 1.2 mm. Tools used during friction stir welding of steel joints were made of W-Re alloy. The joints were subjected to visual inspection and their load bearing capacity was evaluated by means of the tensile strength test with analysis of joint breaking mechanism. Furthermore, the joints were also tested during metallographic examinations. The analysis performed in the study revealed that all the samples of the FSW joints were broken outside the joint area in the base material of the upper sheet metal, which confirms its high tensile strength. Mean load capacity of the joints was 15.8 kN. Macroscopic and microscopic examinations of the joints did not reveal significant defects on the joint surface and in the cross-sections.

In this brief article five bronze fibulae, being exposed in the museum of Şanlıurfa and belonging to the Iron Age, will be presented. At least two of these five were found at Lidar Höyük.