Cytological evaluation of bone marrow smears stained by May-Grünwald Giemsa method was performed. The smears came from 20 fallow deer (Dama dama) 3 days old divided into 2 groups each consisting of 10 animals. The experimental group (E) received intramuscularly selenium and vitamin E at a dose of 3.0 ml (tocopherol acetate – 50 mg, sodium selenite – 0.5 mg, solvent - 1 ml) in the 3rd day of age. The control group (C) did not receive any supplementation or placebo. For hematological analyzes blood was collected three times: on 0, 15th and 25th day of the experiment. Serum concentration of selenium and vitamin E was determined using high perfor- mance liquid chromatography and glutathione peroxidase activity (GSH-Px) by kinetic method. On the 15th day after supplementation, a statistically significant increase in the percentage of erythroblastic cell line was observed in bone marrow smears. At that time, the increase in GSH-Px activity in the E group was also observed, reaching the value of 165.3 U/gHb, which was statisti- cally significant. The percentage of proerythroblasts (8.23% in group E and 5.02% in group C) differed significantly between groups at the 25th day after supplementation. This study revealed that supplementation of selenium and vitamin E resulted in an increase in the number of erythro- cytes to an average of 13.5 (˟ 10¹²/l) in the experimental group on 25th day with a significant increase in hemoglobin to 193 g/l in the experimental group.
The article presents the results of the investigations performed on high manganese austenitic steel which underwent the test of uniaxial tension, with the application of electric current impulses. The application of low voltage impulse alternating current of high intensity during the plastic deformation of the examined steel caused the occurrence of the electroplastic effect, which changed the shape of the stress-strain curve. A drop of flow stress and elongation of the tested material was observed in the case of the application of electric current impulses, in respect of the material stretched without such impulses and stretched at an elevated temperature. The analysis of the morphology of the fractures showed differences between the samples tested under the particular conditions. An analysis of the alloy’s microstructure was also performed under different conditions. The application of electric current impulses can have a significant influence on the reduction of the forces in the plastic forming processes for this type of steel.
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