Search results

Filters

  • Journals
  • Authors
  • Keywords
  • Date
  • Type

Search results

Number of results: 2
items per page: 25 50 75
Sort by:
Download PDF Download RIS Download Bibtex

Abstract

Applications of cold atmospheric plasma/nitric oxide (CAP/NO) gas have recently garnered popularity when treating impaired wound healing in patients with diabetes. In this study, we aimed to investigate the effects of NO gas application for 60 and 120 s on wound healing in diabetic rats. A dorsal excision 3 cm in diameter was performed in 15 diabetic rats; these rats were categorized into the following 3 groups: DC (untreated diabetic control); DNO/60 (exposure to 200 ppm NO gas for 60 s/day); and DNO/120 (exposure to 200 ppm NO gas for 120 s/day). Wound contraction on days 0, 3, 7, 11, and 14 and wound contraction rate between days 0 and 14 were evaluated. On day 14, tissue samples were collected for histopathologic assessment of inflammation, epithelial regeneration, angiogenesis congestion, and collagen fiber organization. Normality of distribution was assessed using the Shapiro-Wilk test, and intergroup comparisons were performed using the Mann-Whitney U test (NPar Test) and the Kruskal-Wallis test (non-parametric ANOVA). Wound contraction during treatment days 7-14 was significantly greater in the NO-treatment groups than in the DC group (p<0.05). The NO60 s and NO120 s groups showed a significantly higher wound contraction rate than the DC group (p=0.033, p=0.049, respectively). Significant differences were noted between the control and NO groups in terms of inflammation (p<0.05) and between the control group and DNO/60 and DNO/120 groups in terms of collagen organization (p<0.05, p<0.01, respectively). Evaluation of epithelialization revealed significant intergroup differences between the control and NO treatment groups (p<0.01). In this study, the application of NO once a day for 60 seconds and 120 seconds in diabetic wounds contributed equally to wound healing.
Go to article

Bibliography

  1. Ahmed R, Augustine R, Chaudhry M, Akhtar UA, Zahid AA, Tariq M, Falahati M, Ahmad IS, Hasan A (2022) Nitric oxide-releasing biomateri-als for promoting wound healing in impaired diabetic wounds: State of the art and recent trends. Biomed Pharmacother 149: 112707.
  2. Anuk T, Öztürk S, Özaydın İ, Kahramanca Ş, Yayla S, Aksoy, Demirkan I (2016) Comparison of Three Fixation Methods for the prevention of wound contractions in diabetic and non-diabetic mice with full-thickness skin excision. Kafkas Univ Vet Fak Derg 22: 647-651.
  3. Bae SH, Bae YC, Nam SB, Choi SJ (2012) A skin fixation method for decreasing the influence of wound contraction on wound healing in a rat model. Arch Plast Surg 39: 457-462.
  4. Bryan NS (2015) Nitric oxide enhancement strategies. Future Sci OA 1: FSO48.
  5. Burgess JL, Wyant WA, Abujamra B, Kirsner RS, Jozic I (2021) Diabetic wound-healing science. Medicina (Kaunas) 57: 1072.
  6. Caskey RC, Liechty KW (2013) Novel animal models for tracking the fate and contributions of bone marrow derived cells in diabetic healing. Methods: 99-115.
  7. Förstermann U, Sessa WC (2012) Nitric oxide synthases: Regulation and function. Eur Heart J 33: 829-837
  8. Fridman G, Friedman G, Gutsol A, Shekhter AB, Vasilets VN, Fridman A (2008) Applied Plasma Medicine. Plasma Process Polym 5: 503-533.
  9. Ghaffari A, Jalili R, Ghaffari M, Miller C, Ghahary A (2007) Efficacy of gaseous nitric oxide in the treatment of skin and soft tissue infec-tions. Wound Repair Regen 15: 368-377.
  10. Ghaffari A, Miller CC, McMullin B, Ghahary A (2006) Potential application of gaseous nitric oxide as a topical antimicrobial agent. Nitric Ox-ide 14: 21-29.
  11. Ghaffari A, Neil DH, Ardakani A, Road J, Ghahary A, Miller CC (2005) A direct nitric oxide gas delivery system for bacterial and mammalian cell cultures. Nitric Oxide 12: 129-140.
  12. Gronbach M, Mitrach F, Lidzba V, Müller B, Möller S, Rother S, Schulz-Siegmund M (2020) Scavenging of Dickkopf-1 by macromer-based biomaterials covalently decorated with sulfated hyaluronan displays pro-osteogenic effects. Acta Biomater 114: 76-89.
  13. Güngör GÇ, Gültekin Ç, Kükner A, Etikan İ, Temizel M, Özgencil FE (2022) Effect of topical insulin and ozonized cream for the treatment of full-thickness dermal burn injuries: A clinical and histopathological study in diabetic rats. Pak Vet J 42: 229-235.
  14. Krausz A, Friedman AJ (2015) Nitric oxide as a surgical adjuvant. Future Sci OA 1: FSO56.
  15. Luo JD, Chen AF (2005) Nitric oxide: A newly discovered function on wound healing. Acta Pharmacol Sin 26: 259-264.
  16. Malone-Povolny MJ, Maloney SE, Schoenfisch MH (2019) Nitric oxide therapy for diabetic wound healing. Adv Healthc Mater 8: e1801210.
  17. Grada A, Mervis J, Falanga V (2018) Research techniques made simple: Animal models of wound healing. J Invest Dermatol 138: 2095-2105..
  18. Mieczkowski M, Mrozikiewicz-Rakowska B, Kowara M, Kleibert M, Czupryniak L (2022) The problem of wound healing in diabetes-from mo-lecular pathways to the design of an animal model. Int J Mol Sci 23: 7930.
  19. Miersch S, Espey MG, Chaube R, Akarca A, Tweten R, Ananvoranich S, Mutus B (2008) Plasma membrane cholesterol content affects nitric oxide diffusion dynamics and signaling. J Biol Chem 283: 18513-18521.
  20. Miller CC, Miller MK, Ghaffari A, Kunimoto B (2004) Treatment of chronic nonhealing leg ulceration with gaseous nitric oxide: A case study. J Cutan Med Surg 8: 233-238.
  21. Pekshev AV, Shekhter AB, Vagapov AB, Sharapov NA, Vanin AF (2018) Study of plasma-chemical NO-containing gas flow for treatment of wounds and inflammatory processes. Nitric Oxide 73: 74-80.
  22. Powers JG, Higham C, Broussard K, Phillips TJ (2016) Wound healing and treating wounds: Chronic wound care and management. J Am Acad Dermatol 74: 607-625.
  23. Moeen Rezakhanlou A, Miller C, McMullin B, Ghaffari A, Garcia R, Ghahary A (2011) Gaseous nitric oxide exhibits minimal effect on skin fibroblast extracellular matrix gene expression and immune cell viability. Cell Biol Int 35: 407-415.
  24. Shekhter AB, Pekshev AV, Vagapov AB, Telpukhov VI, Panyushkin PV, Rudenko TG, Fayzullin AL, Sharapov NA, Vanin AF (2019) Physi-cochemical parameters of NO-containing gas flow affect wound healing therapy. An experimental study. Eur J Pharm Sci 128: 193-201.
  25. Shekhter AB, Serezhenkov VA, Rudenko TG, Pekshev AV, Vanin AF (2005) Beneficial effect of gaseous nitric oxide on the healing of skin wounds. Nitric Oxide 12: 210-219.
  26. Wu M, Lu Z, Wu K, Nam C, Zhang L, Guo J (2021) Recent advances in the development of nitric oxide-releasing biomaterials and their application potentials in chronic wound healing. J Mater Chem B 9: 7063-7075.
  27. Yang Y, Qi PK, Yang ZL, Huang N (2015) Nitric oxide based strategies for applications of biomedical devices. Biosurf Biotribol 1: 177-201.
  28. Young LH, Ikeda Y, Lefer AM (2001) Caveolin-1 peptide exerts cardioprotective effects in myocardial ischemia-reperfusion via nitric oxide mechanism. Am J Physiol Heart Circ Physiol 280: H2489-H2495.
Go to article

Authors and Affiliations

G. Tatlıcıoğlu
1
A. Çürükoğlu
1
G. Akan
2
G. Yeşilovalı
1
G. Öğütçü
3
R. Aktaş
1
A. Kükner
3
M. Temizel
4
Z.K. Sarıtaş
5
F.E. Özgencil
1

  1. Surgery Department, Near East University, Faculty of Veterinary Medicine, Yakın Doğu St, VIC: 99138 Nicosia / TRNC Mersin 10 – Turkey
  2. DESAM Institute, Near East University, Yakın Doğu St, VIC: 99138 Nicosia / TRNC Mersin 10 – Turkey
  3. Histology Department, Near East University, Faculty of Medicine, Yakın Doğu St, VIC: 99138 Nicosia / TRNC Mersin 10 – Turkey
  4. Experimental Animal Research Center, Near East University Faculty of Veterinary Medicine, Yakın Doğu St, VIC: 99138 Nicosia / TRNC Mersin 10- Turkey
  5. Surgery Department, Afyon Kocatepe University, Faculty of Veterinary Medicine, ANS Campus, Erenler, Afyonkarahisar/Turkey

This page uses 'cookies'. Learn more