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Abstract

The paper deals with the preparation and measurement of an experimental polymer graphite cathode that

seems to be a promising and cheap source of electrons utilizing cold field-emission in high- and ultra-high

vacuum. Polymer graphite seems to be a proper material as it contains a large amount of hybridized carbon

with a low degree of surface oxidation and silicon monoxide (SiO). Within the frame of this work, a special

experimental method of tip preparation has been designed and tuned. This method is based on ion milling

inside a dual-beam electron microscope enabling to obtain ultra-sharp tips of a diameter smaller than 100 nm

with a predefined opening angle. The charge transport within experimental samples is evaluated based on

results provided by the noise spectroscopy of the total emission current in the time and frequency domains.

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Authors and Affiliations

Alexandr Knápek
Miroslav Horáček
Jana Chlumská
Tomáš Kuparowitz
Dinara Sobola
Josef Šikula
Keywords rabbit RHDV2 Poland
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Abstract

In this paper we present the first cases of rabbit haemorrhagic disease virus 2 (RHDV2 – GI.2) in Poland. The virus was detected in liver samples of RHD-suspected rabbits from Lodzkie and west Pomeranian voivodeships. In both cases, the typical clinical symptoms of the disease were observed despite the fact that the rabbits were previously vaccinated against RHD. In order to extend the analysis of the RHDV2 strain infecting the rabbits, the entire VP60 and NSP genes were amplified and sequenced. The results of rRT-PCR assay have shown that tested RHDV samples were positive for the presence of RHDV2. In the phylogenetic analysis of vp60gene the first Polish RHDV isolates (RED 2016 and VMS 2017) clustered together with the reference RHDV2, meaning they represent new evolutionary RHDV linkeages. The first Polish RHDV2 isolates showed about 97% nucleotide sequence identity with the reference RHDV2 strains and approximately 18% difference from classic RHDV and RHDVa variants.

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Authors and Affiliations

A. Fitzner
W. Niedbalski

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