Present study describes about the effect of coolant water flow rate and coolant water temperature underside cooling slope on structural characteristics of casted AZ91 Mg alloy. Here, over the cooling slope, hot melt flows from top to bottom. Additionally, under the cooling slope, coolant water flows from bottom to top. Slurry gets obtained at bottom of cooling slope by pouring AZ91 Mg melt from top of the slope. Coolant water flow rate with coolant water temperature underside cooling slope warrant necessary solidification and shear to obtain AZ91 Mg slurry. Specifically, slurry at 5 different coolant water flow rates (4, 6, 8, 10, 12 lpm) and at 5 different coolant water temperatures (15, 20, 25, 30, 35°C) underside cooling slope are delivered inside metal mould. Modest coolant water flow rate of 8 lpm with coolant water temperature of 25°C (underside cooling slope) results fairly modest solidification that would enormously contribute towards enhanced structural characteristics. As, quite smaller/bigger coolant water flow rate/temperature underside cooling slope would reason shearing that causes inferior structural characteristics. Ultimately, favoured microstructure was realized at 8 lpm coolant water flow rate and 25°C coolant water temperature underside cooling slope with grain size, shape factor, primary α-phase fraction and grain density of 63 µm, 0.71, 0.68 and 198, respectively. Correspondingly, superior mechanical properties was realized at 8 lpm coolant water flow rate and 25°C coolant water temperature underside cooling slope with tensile strength, elongation, yield strength and hardness of 250 MPa, 8%, 192 MPa and 80 HV, respectively.

Myxomatous mitral valve disease (MMVD) is a cardiac condition commonly found in older dogs. The disease process can lead to heart failure (HF). In HF, an increase of reactive oxygen species (ROS) and abnormal mitochondrial activity, as well as apoptosis, have been reported. Humanin (HN) is a polypeptide that has a cardioprotective effect against apoptosis and oxidative stress. The purposes of this study were (1) to investigate the potential role of plasma HN as a cardiac biomarker to predict disease progression of MMVD, and (2) to compare plasma HN concentrations with plasma NT-pro BNP concentrations. Thirty-one dogs were included in the study. The dogs were separated into four groups: Group 1 was healthy dogs (n = 8), Group 2 was MMVD class B (n = 8), Group 3 was MMVD class C (n = 8), and Group 4 was MMVD class D (n = 7). All dogs were given a physical examination, thoracic radiography, echocardiography, and samples of their blood were collected for hematology and blood chemistry analysis. Levels of plasma HN and plasma NT-proBNP were also investigated. The results showed that plasma HN levels were lower in the dogs with MMVD and that lower plasma HN levels were associated with greater severity of MMVD-induced HF. It was possible to observe changes in plasma HN levels at a less severe disease stage than plasma NT-proBNP in dogs with MMVD. These findings sug- gest that a decreased plasma HN level can be used as a biomarker to identify dogs with MMVD -induced HF.