Observations on the incidence, harmfulness and some elements of Cameraria ohridella control on white horse chestnut (Aesculus hippocastanum L.) were carried out in Wrocław, Poland, in 2001–2003. Three generations of the pest developed on A. hippocastanum. The leaf infestation by pest’s larvae increased systematically throughout the spring-summer season. Cultural control by removing the fallen leaves was suggested. Using this procedure, the abundance of the pest was considerably reduced, therefore, the percentage of the leaf damage was lower and the trees lost these leaves later in the season.
Observations on the development of the horse chestnut leafminer on red horse chestnut (Aesculus x carnea H.) were carried out in Wrocław, Lower Silesia, Poland, in 2001–2003. Three generations of the pest were recorded to lay eggs on the red horse chestnut leaves. Although females of each generation deposited eggs abundantly, the hatching larvae died after a short period of feeding in the plant’s leaves and the species did not complete its development on this tree. Mostly L1 and L2 larvae were found inside the leaf mines. The observed leaf damage was, therefore, negligible.
This study set out to investigate, for the first time, the distribution and colocalization pattern of cocaine-and amphetamine-regulated transcript (CART) and one of the calcium binding-proteins: parvalbumin (PV) in the chinchilla’s hippocampus proper (HP). HP, consisting of Ammon’s horn (CA) and the dentate gyrus (DG), is an important component of the limbic system, involved in learning and memory processes. CA showed a higher immunoreactivity of CART (-IR) compared to DG. CART-IR neurons were mainly observed in the molecular layer of DG and in the pyramidal layer of CA. CART-IR fibers were present in the granular layer; in the hilus numerous mossy fibers were detected, while in the molecular layer CART-IR fibers were not found. In all CA fields (CA1-CA3), CART-IR fibers were only present in the lacuno- sum-molecular layer. Immunofluorescence with double- labeling showed that only CART-IR cells stained positive for PV, whereas in CART-IR fibers there was no PV-positive reaction. Our research supplements missing knowledge about the distribution and colocalization pattern of CART with PV in the chinchilla’s hippocampus, and also provides a better understanding of the similarities and differences among individuals of the same species and also with other mammals.