The aim of this study was to determine the hardness and reduced modulus of elasticity of juvenile wood of Scots pine (Pinus sylvestris L.) using the nanoindentation method, and then to compare the results obtained with those of mature wood. The hardness of juvenile pine wood determined by means of the nanoindentation method was 0.444 GPa while for mature wood it was 0.474 GPa. Statistically significant differences between the values were found. The reduced modulus of elasticity in juvenile wood was 14.0 GPa and 16.4 GPa in mature wood. Thus, the hardness values obtained were about 7% higher, while the modulus of elasticity was 17% higher in mature wood. All determinations were made in the S2-layer of the secondary cell wall.