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Abstract

Variability of stress proteins concentration in caged carp exposed to transplantation experiment model dam reservoir was caused only by natural (climatic and biological) conditions. Thus, the reference data of stress proteins concentration range in young carp individuals were obtained. Metallothionein, HSP70 and HSP90 protein concentrations as biomarkers were assayed in the livers, gills and muscles of six-month-old (summer) or nine-month-old (autumn) carp individuals in relation to the site of encaging, season (summer or autumn), the term of sampling (1, 2 or 3 weeks after the transplantation) and tissue. Physicochemical analyses of the condition of water as well as pollution detection were conducted during each stage of the experiment. As the result of this study, the range of the variability of the stress protein concentration in young carp individuals was obtained. According to the analyses of the aquatic conditions of a reservoir with no detectable pollutants, we conclude that the variability in the stress protein concentration levels in the groups that were compared is solely the result of the natural conditions. Future regular monitoring of the reservoir using the transplantation method and young carp individuals will be both possible and reliable. Moreover, the range of variability in the stress protein concentrations that were measured in the young C. carpio individuals acquired from the model dam reservoir in relation to all of the studied factors may be applied in the monitoring of any other similar reservoir.
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Authors and Affiliations

Agnieszka Babczyńska
1
Monika Tarnawska
1
Piotr Łaszczyca
1
ORCID: ORCID
Paweł Migula
1
Bartosz Łozowski
1
Andrzej Woźnica
1
Ilgiz Irnazarow
2
ORCID: ORCID
Maria Augustyniak
1

  1. Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Poland
  2. Institute of Ichtyobiology and Aquaculture in Gołysz, Polish Academy of Sciences, Poland
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Abstract

Ecotoxicological biotests were applied in order to evaluate their suitability as early warning systems in the continuous monitoring of lowland shallow dam reservoirs located in Central Europe. The following biotests were used: Daphtoxkit F™magna, Algaltoxkit F™, Ostracodtoxkit F, Phytotoxkit and MARA Test. The experiment was conducted from July 2010 to December 2012 in Goczalkowice Reservoir (the Vistula River, Poland), serving as a model. For the analysis, 41 out of 52 measured water indices were used to assess its toxicity to living organisms. The results of biotests were correlated with 41 hydrochemical indices of water quality. The pattern of relationships among the result of biotest and hydrochemical indices as well as Factor Analysis (FA) and Primary Component Analysis (PCA) revealed that: i) signs of ecotoxicity detected with biotests were associated with either low fl ow periods or spring surface runoff of water; ii) single events of increased ecotoxicity in the depression areas behind saddle dam pump stations appearedafter high fl ow periods; iii) elevated toxicity was accompanied by high concentrations of dissolved and suspended substances; iv) FA and PCA demonstrated correlations among the results of biotests and damming parameters, water conductivity, alkali and transitory metal metals (Ca, Fe, Cu, Zn), and several forms of nitrogen phosphorous and carbon compounds concentration. The relationships suggest that batteries of biotests may serve as a cost-eff ective tool for continuous monitoring of water quality in dam reservoirs and can detect eff ects of extreme hydrologic events, local toxic discharges, and signs of the trophic status of the reservoirs
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Authors and Affiliations

Piotr Łaszczyca
1
ORCID: ORCID
Mirosław Nakonieczny
2
ORCID: ORCID
Maciej Kostecki
3
ORCID: ORCID

  1. Retired university professor, University of Silesia in Katowice, Poland
  2. University of Silesia in Katowice, Poland
  3. Institute of Environmental Engineering Polish Academy of Sciences, Zabrze, Poland

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