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The aim of the study was to analyse mechanical properties and microstructure of joints obtained using friction stir welding (FSW) technology. The focus of the study was on overlap linear FSW joints made of 1.4541 DIN 17441 steel sheets with thickness of 1.2 mm. Tools used during friction stir welding of steel joints were made of W-Re alloy. The joints were subjected to visual inspection and their load bearing capacity was evaluated by means of the tensile strength test with analysis of joint breaking mechanism. Furthermore, the joints were also tested during metallographic examinations. The analysis performed in the study revealed that all the samples of the FSW joints were broken outside the joint area in the base material of the upper sheet metal, which confirms its high tensile strength. Mean load capacity of the joints was 15.8 kN. Macroscopic and microscopic examinations of the joints did not reveal significant defects on the joint surface and in the cross-sections.

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Authors and Affiliations

W. Więckowski

P. Lacki

J. Adamus

Immunoreactivity of acetylcholinesterase and M1 muscarinic receptors in the hippocampus and striatum of rats treated with Rebaudioside A

K. Rycerz J. Jaworska-Adamu A. Krawczyk M.B. Arciszewski

Polish Journal of Veterinary Sciences | 2022 | vol. 25 | No 2 | 303-310 | DOI: 10.24425/pjvs.2022.141815

Keywords rebaudioside A stevia acetylcholinesterase hippocampus striatum rat

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Abstract

Rebaudioside A (RebA) is a steviol glycoside used for production of sweeteners. It was shown that the glycosides affect memory and learning processes. The aim of the study was to investigate neurons immunoreactive for acetylcholinesterase (AChE) and M1 muscarinic receptors (mAChRs-M1) of the hippocampal CA1 and CA3 fields and striatal caudateputamen (CP) and globus pallidus (GP) in rats receiving RebA. RebA was administrated to adult rats for 45 days in dilutions of 1 mg and 2 mg RebA/ml water. Indirect peroxidase-antiperoxidase immunohistochemical reaction was conducted on frontal sections containing the hippocampus and striatum with use of antibodies against AChE and mAChRs-M1. Immunoreactive for the studied proteins neurons were morphologically and morphometrically assessed in hippocampal CA1 and CA3 fields and in CP and GP. Microscopic observations did not reveal significant changes in morphology of immunoreactive neurons, which suggests no neurotoxic effect of the studied glycoside on these cells. Morphometric analyses revealed an increase in the density of AChE and mAChRs-M1 immunoreactive neurons. A decrease in reaction intensity of AChE-positive neurons was also demonstrated in the hippocampal CA1 field and in GP. In contrast, an increase in reaction intensity of mAChRs-M1-positive neurons was found in CA1, CA3 fields and in CP and GP. The results of our preliminary studies indicate that RebA administrated to rats has an impact on cholinergic neurons in the studied area. The results suggest a possible increase in the activity of the cholinergic system, responsible for memory and learning processes, after administration of RebA.

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