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Abstract

Based on the rolling bearing vibration measurement principle in ISO standard, a nonlinear dynamic model of ball bearing is built and motion equations of the inner ring, outer ring, and rolling elements are derived by using Lagrange’s equation. The ball bearing model includes the influence of waviness, rotational speed, external load, arbor supporting stiffness and arbor eccentricity. Ball bearing high-speed vibration tests are performed and used to verify the theoretical results. Simulated results showed that specific waviness orders produced the principal frequencies that were proportional to rotational speed. Rotational speed mainly affected the value of the natural frequency of the bearing system, and RMS (Root Mean Square) of the full band had a great fluctuation with the increase of rotational speed. In the experiment, spectrum and RMS of 2fs-30 kHz (fs: the rotational frequency of inner ring/arbor) under high speed could include not only the influence of rotational speed but also principal frequencies produced by waviness, which could cover the part of requirements of the standard bearing vibration measurement.

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Authors and Affiliations

P.P Hou
L.Q. Wang
Q.Y. Peng
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Abstract

Alveolar macrophages (AMs) are not only important immune cell of the host, but also important target cell of a variety of respiratory pathogens. They play an important role in defense against pathogen invasion and in maintaining tissue balance. Interferon (IFN)-γ is a well known multipotent cytokine that has antiviral and antibacterial immune activity and enhances antigen presentation. To better reveal the biological processes of porcine AMs activated by IFN-γ, transcriptomic analysis was performed using Illumina HiSeqTM technique. The results identified 2,248 differentially expressed genes (DEGs), of which 753 were upregulated and 1,495 were downregulated, in porcine AMs 12 h after IFN-γ stimulation, compared with mock-treated porcine AMs. A gene ontology function enrichment analysis of these DEGs indicated that these genes were significantly enriched in functional clusters such as immune response, defense response, and intracellular signaling cascades. Analyzing the Kyoto Encyclopedia of Genes and Genomes pathways of the DEGs showed that these genes are mainly involved in cytokine–cytokine receptor interactions, alpha linolenic acid metabolism, and the RIG-I-like receptor signaling pathway. This study shows that a massive gene expression change occurred in porcine AMs following IFN-γ stimulation, which is critical for understanding the mechanisms of IFN-γ-mediated macrophage activation and immune regulation.
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Authors and Affiliations

Q. Liu
1
H.-Y. Wang
1

  1. Nanchong Key Laboratory of Disease Prevention, Control, and Detection in Livestock and Poultry, Nanchong Vocational and Technical College, Nanchong 637131, China
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Abstract

This work evaluates the influence of gate drive circuitry to cascode GaN device’s switching waveforms. This is done by comparing three PCBs using three double-pulse-test (DPT) with different gate driving loop design. Among important parasitic elements, source-side inductance shows a significant impact to gate-source voltage waveform. A simulation model based on experimental measurement of the cascode GaNFET used in this work is modified by author. The simulation model is implemented in a synchronous buck converter topology and hereby to assess the impact of gate driving loop of cascode GaN device in both continuous conduction mode (CCM) and critical conduction mode (CRM). Apart from simulation, a synchronous buck converter prototype is presented for experimental evaluation, which shows a 99.15% efficiency at 5A under soft-switching operation (CRM) with a 59ns dead-time.
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Bibliography

  1.  Power Electronics UK and CSA CATAPULT, “Opportunities and Challenges of Wide Band Gap Power Devices”, pp. 1–8, 2020.
  2.  E.A. Jones, F.F. Wang, and D. Costinett, “Review of Commercial GaN Power Devices and GaN-Based Converter Design Challenges”, IEEE J. Emerg. Sel. Top. Power Electron. 4(3), 707–719 (2016).
  3.  H. Jain, S. Rajawat, and P. Agrawal, “Comparision of wide band gap semiconductors for power electronics applications”, 2008 Int. Conf. Recent Adv. Microw. Theory Appl. Microw, 2008, pp. 878–881.
  4.  S. Chowdhury, Z. Stum, Z. Da Li, K. Ueno, and T.P. Chow, “Comparison of 600 V Si, SiC and GaN power devices”, Mater. Sci. Forum 778–780, pp. 971–974 (2014).
  5.  A. Taube, M. Sochacki, J. Szmidt, E. Kamińska, and A. Piotrowska, “Modelling and Simulation of Normally-Off AlGaN/GaN MOS- HEMTs”, Int. J. Electron. Telecommun. 60(3), 253–258 (2014).
  6.  B.N. Pushpakaran, A.S. Subburaj, and S.B. Bayne, “Commercial GaN-Based Power Electronic Systems: A Review”, J. Electron. Mater. 49(11), 6247–6262 (2020).
  7.  C.T. Ma and Z.H. Gu, “Review of GaN HEMT applications in power converters over 500 W”, Electronics 8(12), 1401 (2019).
  8.  H. Jain, S. Rajawat, and P. Agrawal, Comparision of wide band gap semiconductors for power electronics applications, 2008.
  9.  H. Umeda et al., “High power 3-phase to 3-phase matrix converter using dual-gate GaN bidirectional switches”, 2018 IEEE Applied Power Electronics Conference and Exposition (APEC), San Antonio, USA, 2018, pp. 894‒897, doi: 10.1109/APEC.2018.8341119.
  10.  K. Nowaszewski and A. Sikorski, “Predictive current control of three-phase matrix converter with GaN HEMT bidirectional switches”, Bull. Pol. Acad. Sci. Tech. Sci. 68(4), 1077–1085 (2020).
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  19.  K. Umetani, K. Yagyu, and E. Hiraki, “A design guideline of parasitic inductance for preventing oscillatory false triggering of fast switching GaN-FET”, IEEJ Trans. Electr. Electron. Eng. 11(52), S84–S90 (2016).
  20.  T. Ibuchi and T. Funaki, “A study on parasitic inductance reduction design in GaN-based power converter for high-frequency switching operation”, 2017 International Symposium on Electromagnetic Compatibility – EMC EUROPE, Angers, 2017, pp. 1‒5, doi: 10.1109/ EMCEurope.2017.8094824.
  21.  B. Sun, Z. Zhang, and M.A.E. Andersen, “Research of low inductance loop design in GaN HEMT application”, IECON 2018 – 44th Annual Conference of the IEEE Industrial Electronics Society, Washington, USA, 2018, pp. 1466‒1470, doi: 10.1109/IECON.2018.8591732.
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Authors and Affiliations

Q.Y. Tan
1
E.M.S. Narayanan
1

  1. Department of Electronic and Electrical Engineering, The University of Sheffield, S1 3JD, UK
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Abstract

The public transport service is highly essential to meet the demand due to a rapidly growing population and mobility. Thus providing public service and improve its service becomes an urgent need in recent years. In Iraq, the Bus system represents the backbone in public transportation, which is based mainly on highway infrastructure. To meet the growing mobility needs, enhancing public service provided only by bus routes is essential. Measuring bus route performance represents one of the crucial transit research topics in the last recent years. The current study tries to investigate the urban public route's efficiency utilizing the "data envelopment analysis (DEA)" technique. To analyze route performance, DEA is using, and performance measures include route design, cost, service, operation, and comfort efficiency are selected and calculated for different routes. Efficiency and effectiveness are the output of this process. Bus company owners can also use the results of this study to improve their services, attract new customers, and better manage their resources.

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Authors and Affiliations

Noor M. Asmael
Mohanned Q. Waheed
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Abstract

Despite over 40 years of research on the human immunodeficiency virus type 1 (HIV-1) vaccine, we still lack a considerable progress. Equine infectious anemia virus (EIAV) is a lentivirus in the Retroviridae family, akin to HIV-1 in genome structure and antigenicity. EIA is an important infectious disease in equids, characterized by anemia, persistent infection, and repeated fevers. The EIAV attenuated vaccine in China is the only lentiviral vaccine used on a large scale. Elucidating the mechanism of waning and induction of protective immunity from this attenuated vaccine strain will provide a critical theoretical basis and reference point for vaccine research, particularly in the development of lentivirus vaccines, with far-reaching scientific value and social significance. In this paper, we summarize the information related to EIAV integration site selection, particularly for the Chinese EIAV attenuated vaccine strains on the equine genome. This may improve our mechanistic understanding of EIAV virulence reduction at the host genome level. The obtained data may help elucidate the biological characteristics of EIAV, particularly the Chinese attenuated EIAV vaccine strain, and provide valuable information regarding retroviral infections, particularly lentiviral infection and associated therapeutic vectors.
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Authors and Affiliations

Y.-Y. Yu
1
M.-S. Xu
2
H. Liang
1
H.-Y. Wang
1
C.-Q. Yu
3
Q. Liu
1

  1. Nanchong Key Laboratory of Disease Prevention, Control and Detection in Livestock and Poultry, Nanchong Vocational and Technical College, Nanchong 637131, China
  2. Chongqing Three Gorges Vocational College, Wanzhou 404155, China
  3. School of Advanced Agricultural Sciences, Yibin Vocational Technical College, Yibin 644003, China
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Abstract

With the improvement of people’s living standards and rapid economic development, the incidence of diabetes mellitus (DM) is increasing in most parts of the world. DM presents an important potential threat to human health. In the present study, a model of diabetes in female mice was established, and fasting blood glucose was detected at week 4, after which the biochemical profiles were evaluated by histopathological analysis. The success rate of modeling in the normal control (NC) group and the low/ middle/high-dose streptozotocin (STZ) group were 0, 0, 25% and 60%, respectively. In the middle-dose and high-dose STZ groups, the liver index was increased significantly compared with the NC group (p<0.05). The blood biochemical indicators of total cholesterol and low density lipoprotein cholesterol in three STZ injection groups were as follows: alanine aminotransferase and aspartate transaminase in middle- and high-dose STZ groups, high-density lipoprotein cholesterol and serum creatinine in the high-dose STZ group, and blood urea nitrogen in the middle-dose STZ group were significantly increased (p<0.05). The level of total triglycerides was lower, obviously, in the high-dose STZ group than in the NC group (p<0.05). The mice showed marked steatosis, green-dyed fiber tissue coloring in varying degrees, and the contour of the hepatic lobules basically disappeared in STZ injection groups. The results suggest that to establish a diabetes model for female ICR mice, the optimum dose of STZ is 100 mg/kg.
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Authors and Affiliations

R. Guo
1 2
J. Dong
3
D.Q. Wang
3
Y.F. Gu
1 2

  1. State Key Laboratory for Diagnosis and Treatment of Infectious Disease, National Clinical Research Center for Infectious Disease, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China
  2. Jinan Microecological Biomedicine Shandong Laboratory, No. 3716 Qingdao Road, Huaiyin District, Jinan City, Shandong Province, Solutia City Light West Building, 21F, Shandong Laboratory of Microecological Biomedicine, Jinan 250117, China
  3. Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
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Abstract

Thymus, an important central immune organ in pigs, is the site of T lymphocyte development and maturation and an important target organ for infection and replication of various pathogens. Highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) infection results in severe thymic atrophy in piglets. This study aimed to explore the effects of HP-PRRSV on the thymic structure of piglets to elucidate the pathogenesis of thymic atrophy induced by HP-PRRSV. In this study, histopathological techniques and immunofluorescence double staining techniques were used to analyze thymic tissues infected by HP-PRRSV to explore the structural changes of thymus caused by the viral infection and its target cell types. An antibody of cluster of differentiation (CD) 3 (CD3), CD20, CD80, or calgranulin + calprotectin was applied to identify T cells, B cells, dendritic cells (DCs), and macrophages, respectively. The results indicated that a variety of cell components in the thymic tissue were diffusely damaged after viral infection. In the infected thymic tissue, CD80- or calgranulin + calprotectin- -labeled cells supported the HP-PRRSV infection, whereas CD3-labeled T cells and CD20- -labeled B cells did not support the viral infection. The results showed that HP-PRRSV caused the reduction of visible cell components in the thymic tissue, and the virus attacked CD80- and calgranulin + calprotectin-positive cells (such as DCs and macrophages) in the thymic tissue, which played an important role in the pathogenesis of thymus atrophy. These results lay the foundation for elucidating the immunosuppression of piglets after infection with HP-PRRSV.
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Authors and Affiliations

Q. Liu
1
Y.-Y. Yu
1
H.-Y. Wang
1

  1. Nanchong Key Laboratory of Disease Prevention, Control and Detection in Livestock and Poultry, Nanchong Vocational and Technical College, Nanchong 637131, China
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Abstract

African swine fever (ASF) is an acute, hemorrhagic, and devastating viral infectious disease that causes important economic losses to the swine industry. Currently, there are no effective vaccines or drugs available. Epigenetic mechanisms, especially cytosine methylation of cytosine- -phosphate-guanine (CpG) islands, have a significant impact on the life cycle of several viruses. Hence, drugs targeting DNA methylation may potentially be used for the treatment of ASF. Here, we selected the inner core, core shell, inner membrane, capsid, and external envelope membrane, to analyze the characteristics of CpG islands in the ASF virus (ASFV) genomes. Furthermore, we analyzed the promoters and CpG islands in the upstream regions of these genes. Results showed that the CpG islands of seven genes were conserved in the genomes of two genotype of ASFV strains, whereas the CpG islands of other genes were relatively conserved (ASFV strains differed mainly in the quantity of CpG islands). The different distribution of CpG islands in the genomes of different ASFV strains may affect their methylation status, which may in turn affect the regulation of viral gene expression, leading to different clinical outcomes. In addition, the predicted promoter regions based on the upstream sequences of most genes overlapped with CpG island positions. Methylation of the binding sites of the promoter regions inhibits the binding of the transcription factors to the promoters, thus inhibiting the activation of the promoters and limiting the synthesis of viral proteins. The results of this study provide a basis for exploring new antiviral therapeutic strategies from an epigenetic perspective.
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Authors and Affiliations

Y.-Y. Yu
1
M.-S. X
2
Q. Liu
1

  1. Nanchong Key Laboratory of Disease Prevention, Control and Detection in Livestock and Poultry, Nanchong Vocational and Technical College, Nanchong 637131, China
  2. Chongqing Three Gorges Vocational College, Wanzhou 404155, China
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Abstract

Several recent earthquakes have indicated that the design and construction of bridges based on former seismic design provisions are susceptible to fatal collapse triggered by the failure of reinforced concrete columns. This paper incorporates an experimental investigation into the seismic response of nonductile bridge piers strengthened with low-cost glass fiber reinforced polymers (LC-GFRP). Three full-scale bridge piers were tested under lateral cyclic loading. A control bridge pier was tested in the as-built condition and the other two bridge piers were experimentally tested after strengthening them with LC-GFRP jacketing. The LC-GFRP strengthening was performed using two different configurations. The control bridge pier showed poor seismic response with the progress of significant cracks at very low drift levels. Test results indicated the efficiency of the tested strengthening configurations to improve the performance of the strengthened bridge piers including crack pattern, yield, and ultimate cyclic load capacities, ductility ratio, dissipated energy capacity, initial stiffness degradation, and fracture mode.

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Authors and Affiliations

K. Rodsin
Q. Hussain
P. Joyklad
A. Nawaz
H. Fazliani
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Abstract

To study the difference in seismic vulnerability of multiple typical structures in multiple intensity zones, the seismic damage of 7099 buildings of Dujiangyan masonry structure (MS), reinforced concrete structure (RC) and bottom frame seismic wall masonry (BFM) in the 2008 Wenchuan earthquake in China is summarized and analysed. First, a statistical analysis of the data is carried out, the empirical seismic vulnerability matrix and model curves are established by considering the number of storeys, the age and the fortification factors.The vulnerability curves of the cumulative exceeding probability of the empirical seismic damage and the grade of the seismic damage in multiple intensity zones are shown. The mean damage index vulnerability matrix model is proposed and verified using the empirical seismic damage matrix of typical structures.

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Authors and Affiliations

S.Q. Li
T.L. Yu
Y.S. Chen
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Abstract

In recent years, GaN-based light-emitting diode (LED) has been widely used in various applications, such as RGB lighting system, full-colour display and visible-light communication. However, the internal quantum efficiency (IQE) of green LEDs is significantly lower than that of other visible spectrum LED. This phenomenon is called “green gap”. This paper briefly describes the physical mechanism of the low IQE for InGaN/GaN multiple quantum well (MQW) green LED at first. The IQE of green LED is limited by the defects and the internal electric field in MQW. Subsequently, we discuss the recent progress in improving the IQE of green LED in detail. These strategies can be divided into two categories. Some of these methods were proposed to enhance crystal quality of InGaN/GaN MQW with high In composition and low density of defects by modifying the growth conditions. Other methods focused on increasing electron-hole wave function overlap by eliminating the polarization effect.

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Authors and Affiliations

Q. Zhou
M. Xu
H. Wang
ORCID: ORCID
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Abstract

Composition effect on electro-optic (EO) properties of a LiNbO3 (LN) single-crystal has been investigated in a Li2O-content range of 47.0–49.95 mol%. Some non-congruent LN crystals with different Li2O-contents were prepared by performing Li-deficient or Li-rich vapour transport equilibration treatments on as-grown congruent LN crystals. Unclamped EO coefficients γ13 and γ33 of these samples were measured by a Mach–Zehnder interferometric method. The measurements show that in the Li-deficient regime both γ13 and γ33 increase by ∼8% as Li2O-content decreases from the congruent 48.6 mol% to the 47.0 mol% in the Li-deficient regime. The feature is desired for the EO application of the Li-deficient crystal. In the near-stoichiometric regime, both γ13 and γ33 reveal a non-monotonic dependence. As the Li2O-content increases from the 48.6 mol%, the EO coefficient decreases. Around Li2O-content 49.5 mol%, a minimum is reached. After that, the EO coefficient recovers slowly. At the stoichiometric composition, it recovers to a value close to that at the congruent point. Comparison shows that different crystal growth methods give rise to different defect structure features and hence different composition effects.

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Authors and Affiliations

J.-Q. Xu
W.-Y. Du
Q. Sun
W.-H. Wong
D.-Y. Yu
E.Y.-B. Pun
D.-L. Zhang
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Abstract

Porcine circovirus type 2 (PCV2) is an economically important swine pathogen and, although small, it has the highest evolution rate among DNA viruses. Commercial PCV2 commercial vaccines are inactivated PCV2 isolates or a subunit vaccine based on the Cap protein of PCV2. Currently, PCV2 VLPs of individual subtype vaccines are available. Although the main prevalent genotype worldwide is PCV2b, the emerging subtype PCV2d subtype is also increasingly associated with PCV disease. The aim of the study was to evaluate the protective efficacy of VLP based on the PCV2b and 2d subtypes against the mixed challenge of two hypotype PCV2 in mice. Thirty-six female SPV Kunming mice were immunized twice with PCV2b and 2d VLPs, then challenged with PCV2b and PCV2d, to assess the immunogenicity and effectiveness of the VLPs. Vaccination of the mice with PCV2b and 2d VLPs elicited a robust antibody response specific for the PCV2. The virus load detected in the 2b and 2d spleen vaccine group was the lowest compared to other groups. Furthermore, there was no pathological damage in the HE stained sections of the 2b and 2d spleen vaccine, and no virus was detected in the immunohistochemical sections. Our data suggest that the mixed PCV2b and 2d VLP vaccine could protect mice from challenge with the mixed infection of PCV2b and PCV2d.
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Authors and Affiliations

X.M. Yuan
1
Q.C. Yuan
1
S.M. Feng
1
Z.B. Deng
1

  1. Laboratory of Animal Disease Prevention and Control and Animal model, Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, College of Veterinary Medicine, Hunan Agricultural University, No. 1 Nongda road, Furong District, Changsha, 410128, People’s Republic of China
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Abstract

In a dairy cow herd consisted of herd of 200 lactating Holstein-Friesian cows and heifers, clinical signs of mastitis in 40 out of 170 animals were observed. Treatments with antibiotics were proved ineffective. Milk bacterial cultures from 15 affected animals revealed Pseudomonas aeruginosa. An autogenous vaccine was administered subcutaneously, twice in a month period, to all adults. Cases of clinical mastitis declined significantly (p<0.0001) during next 3 months.
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Authors and Affiliations

E.J. Petridou
1
I.A. Fragkou
2
S.Q. Lafi
3
N.D. Giadinis
4

  1. Laboratory of Microbiology and Infectious Diseases, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, Greece
  2. Veterinary Service, Ministry of Rural Development and Food, Athens, Greece
  3. Department of Pathology and Animal Health, Faculty of Veterinary Medicine, Jordan University of Science and Technology, P.O. Box 3030, Irbid, Jordan
  4. Clinic of Farm Animals, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, Greece
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Abstract

Montmorillonite (MMT), a natural absorbent agent, has widely been accepted for its antidiarrhea function in human and farm animals; however, its specific physicochemical property limits its biological function in practical use. In the current study, raw MMT was loaded by andrographolide, namely andrographolide loaded montmorillonite (AGP-MMT). The microstructure of AGP-MMT was observed by scanning electron microscope (SEM) and X-ray diffraction (XRD). The effect of AGP-MMT on the growth performance, intestinal barrier and inflammation was investigated in an enterotoxigenic Escherichia coli (ETEC) challenged mice model. The results show that the microstructure of MMT was obviously changed after andrographolide modification: AGP-MMT exhibited a large number of spheroid particles, and floccule aggregates, but lower interplanar spacing compared with MMT. ETEC infection induced body weight losses and intestinal barrier function injury, as indicated by a lower villus height and ratio of villus height/crypt depth, whereas the serum levels of diamine oxidase (DAO), D-xylose and ETEC shedding were higher in the ETEC group compared with the CON group. Mice pretreated with AGP-MMT showed alleviated body weight losses and the intestinal barrier function injury induced by ETEC challenge. The villus height and the ratio of villus height/crypt depth, were higher in mice pretreated with AGP-MMT than those pretreated with equal levels of MMT. Pretreatment with AGP-MMT also alleviated the increased concentration of serum tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β), and the corresponding genes in the jejunum induced by ETEC infection in mice. The protein and mRNA levels of IL-1β were lower in mice pretreated with AGP-MMT than those with equal levels of MMT. The results indicate that AGP-MMT was more effective in alleviating intestinal barrier injury and inflammation in mice with ETEC challenge than MMT.
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Authors and Affiliations

P. Wang
1
L. Li
1
L. Gan
1
Q. Chen
1
H. Qiao
1
W. Gao
1
Y. Zhang
1
J. Wang
1

  1. College of Biology Engineering, Henan University of Technology, Zhengzhou, China
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Abstract

Bovine parvovirus (BPV), bovine coronavirus (BCoV) and bovine parainfluenza virus (BPIV) are common etiologies causing gastrointestinal and respiratory diseases in dairy herds. However, there are few reports on the synchronous detection of BPV, BCoV and BPIV. The present article aimed to develop a quick and accurate RT-PCR assay to synchronously detect BPV, BCoV and BPIV based on their specific probes. One pair universal primers, one pair specific primers and one specific probe was designed and synthesized. After the concentrations of primer and probe and annealing temperature were strictly optimized, the specificity, sensitivity and repeatability of the established triplex probe qRT-PCR were evaluated, respectively. The results showed the recombinant plasmids of pMD18-T-BPV, pMD18-T-BCoV and pMD18-T-BPIV were 554bp, 699bp and 704bp, respectively. The optimal annealing temperature was set at 45.0°C for triplex qRT-PCR. The triplex probe qRT-PCR can only synchronously detect BPV, BCoV and BPIV. Detection sensitivities were 2.0×102, 2.0×102 and 2.0×101 copies/μL for BPV, BCoV and BPIV, being 1000-fold greater than that in the conventional PCR. Detection of clinical samples demon- strated that triplex probe qRT-PCR had a higher sensitivity and specificity. The intra-assay and inter-assay coefficient of variation were lower than 2.0%. Clinical specimens verified that the triplex qRT-PCR had a higher sensitivity and specificity than universal PCR. In conclusion, this triplex probe qRT-PCR could detect only BPV, BCoV and BPIV. Minimum detection limits were 2.0×102 copies/μL for BPV and BCoV, and 2.0×101 copies/μL for BPIV. The sensitivity of this triplex probe qRT-PCR was 1000-fold greater than that in the conventional PCR. The newly qRT-PCR could be used to monitor or differentially diagnose virus infection.

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Authors and Affiliations

J. Geng
Y. Niu
L. Wei
Q. Li
Z. Gong
S. Wei
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Abstract

Porcine parvovirus (PPV) is a major causative agent in reproductive pig disease. The swine industry faces a significant economic and epizootic threat; thus, finding a reliable, quick, and practical way to detect it is essential. In this investigation, recombinant PPV VP2 protein was expressed in the Escherichia coli ( E. coli) expression systems. As shown by electron microscopy (TEM), Western blot, and hemagglutination (HA) assays, the recombinant VP2 protein was successfully assembled into virus-like particles (VLPs) after being expressed and purified. These VLPs had a structure that was similar to that of real PPV viruses and also exhibited HA activity. These VLPs induced high levels of PPV-specific antibody titers in mice after immunization, indicating that the VLPs may be beneficial as potential candidate antigens. VLPs were used as the coating antigens for the VLP ELISA, and the PPV VLPs-based ELISA displayed a high sensitivity (99%), specificity (93.0%) and agreement rate (98.3%) compared to HI assay, and the agreement rate of this ELISA was 97.5% compared to a commercial ELISA kit. Within a plate, the coefficient of variation (CV) was 10%, and between ELISA plates, the CV was 15%. According to a cross-reactivity assay, the technique was PPV-specific in contrast to other viral illness sera. The PPV VLP indirect-ELISA test for PPV detection in pigs with an inactivated vaccine showed that the PPV-positive rate varied among different sample sources from 88.2 to 89.6%. Our results indicate that this ELISA technique was quick, accurate, and repeatable and may be used for extensive serological research on PPV antibodies in pigs.
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Authors and Affiliations

Y. Li
1
Q. Wang
2
W. Yue
1
X. Li
1
Y. Chen
1
Y. Gao
1

  1. Beijing Biomedicine Technology Center of JoFunHwa Biotechnology (Nanjing Co. Ltd.); No.25 Xiangrui Street Daxing District, Beijing 102600 China
  2. State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
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Abstract

Phosphorothioate CpG oligodeoxynucleotides (ODN) are reported to be recognized by the membrane-bound TLR9 and trigger the MyD88-dependent up-regulation of Type I interferons and pro-inflammatory cytokines. Whether plasmids containing multiple CpG motifs stimulate the same signaling pathway is yet to be determined. The present results show that the CpG motifs enrich plasmid pUC18-CpG stimulates RAW 264.7 in vitro, mainly through the TBK1-mediated signaling pathway, causing the up-regulation of IFN-β, and pro-inflammatory cytokines TNF-α and IL-6. When pUC18-CpG is co-administered with the recombinant Echinococcus granulosus antigen, the antigen-specific antibody titers are markedly increased compared to the Quil-A adju- vanted group. Antigen specific cytokine quantification shows that cytokine profiles from the pUC18-CpG adjuvanted-group are switched to a Th1-biased immune response.

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Authors and Affiliations

J. Wu
Q. Chen
T. Xin
Y. Sun
H. Jia
S.H. Hou
X.Y. Guo
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Abstract

Fruit flies belonging to the Bactrocera dorsalis species complex pose a significant threat to mangoes and other crops in the Philippines and worldwide. Identifying cryptic species within this complex is challenging, particularly when relying solely on morphological analysis. In this study, we sequenced two fragments of the nuclear 18S and ITS2-28S rRNA genes from specimens of Bactrocera dorsalis Hendel collected in the Philippines to assess their applicability for species diagnostics. Subsequent sequencing and analysis revealed that the 18S and 28S rRNA gene fragments matched B. dorsalis sequences in NCBI but also displayed high similarity with other Bactrocera and insect species. On the other hand, sequences of the ITS2 segment showed hits specific to B. dorsalis. Further analysis of the 18S rRNA gene in fruit flies collected from various sources and host plants in the country suggests conserved sequences among Bactrocera samples, irrespective of collection site and host plant species. In conclusion, our findings suggest that, among the tested nuclear DNA fragments, only the ITS2 demonstrates sufficient species-level nucleotide variation for effective use as a molecular diagnostic marker for B. dorsalis identification.
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Authors and Affiliations

Cris Q. Cortaga
1
ORCID: ORCID
Maria Luz J. Sison
1

  1. Institute of Plant Breeding, College of Agriculture and Food Science, University of the Philippines Los Baños, 4031 College, Laguna, Philippines
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Abstract

The perovskites XBiO3 (X = Al, Ga, In) have been studied in terms of mechanical, optical and thermoelectric behavior for energy harvesting application. Density functional theory is applied to study electronic, optical and thermoelectric properties of the studied materials. Structural, mechanical and thermodynamic stabilities are confirmed from the tolerance factor, Born mechanical stability and formation energy/specific heat capacity. Poisson and Plough ratios show the studied materials are ductile and have ability to withstand pressure. Band structure analysis shows the indirect band gap 3.0/2.1/1.0 eV for ABO/GBO/IBO. A complete set of optical spectra is reported by dielectric constants, refractive index, optical conduction, absorption of light and optical loss energy. Shifting of maximum absorption band to visible region increases the potential of perovskites XBiO3. Transport characteristics are also investigated by electrical conductivity, Seebeck coefficient and figure of merit.

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Authors and Affiliations

Q. Mahmood
S. A. Rouf
E. Algrafy
G. Murtaza
S. M. Ramay
A. Mahmood
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Abstract

The electronic, optical and thermoelectric properties of zirconia-based MgZrO3 oxide have been studied theoretically at a variant pressure up to 25 GPa. Calculations for the formation energy and tolerance factor reveal the thermodynamic and structural stability of MgZrO3. To tune the indirect band gap from to a direct band gap, the optimized structure of MgZrO3 has been subjected to external pressure up to 25 GPa. The optical properties have been discussed in the form of dielectric constant and refraction that brief us about the dispersion, polarization, absorption, and transparency of the MgZrO3. In the end, the thermoelectric parameters have been analyzed at variant pressure against the chemical potential and temperature. The narrow band gap and high absorption in the ultraviolet region increase the demand of the studied oxide for energy harvesting device applications.

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Authors and Affiliations

N.A. Noor
M. Rashid
Q. Mahmood
B. Ul Haq
M.A. Naeem
A. Laref
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Abstract

A high performance distributed sensor system with multi-intrusions simultaneous detection capability based on phase sensitive OTDR (Φ−OTDR) has been proposed and demonstrated. To improve system performance, three aspects have been investigated. Firstly, a model of one−dimensional impulse response of backscattered light and a Monte Carlo method have been used to study how the laser line width affects the system performance. Theoretical and experimental results show that the performances of the system, especially the signal−noise−ratio (SNR), decrease with the broadening of laser linewidth. Secondly, a temperature−compensated fibre Bragg grating with a 3 dB linewidth of 0.05 nm and a wavelength stability of 0.1 pm has been applied as an optical filter for effective denoising. Thirdly, a novel interrogation method for multi−intrusions simultaneous detection is proposed and applied in data denoising and processing. Consequently, benefiting from the three−in−one improvement, a high performance Φ−OTDR has been realized and four simultaneous applied intrusions have been detected and located at the same time along a 14 km sensing fibre with a spatial resolution of 6 m and a high SNR of 16 dB. To the best of our knowledge, this is the most multifunctional Φ−OTDR up to now and it can be used for perimeter and/or pipeline intrusion real−time monitoring.

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Authors and Affiliations

Y. Zhan
Q. Yu
K. Wang
F. Yang
Y. Kong
X. Zhao
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Abstract

Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to be a major public health concern. Nucleocapsid (N) protein is the most abundant structural protein on SARS-CoV-2 virions and induces the production of antibodies at the early stage of infection. Large-scale preparation of N protein is essential for the development of immunoassays to detect antibodies to SARS-CoV-2 and the control of virus transmission. In this study, expression of water-soluble N protein was achieved through inducing protein expression at 25°C with 0.5 mM IPTG for 12 h. Western blot and ELISA showed that recombinant N protein could be recognized by sera collected from subjects immunized with Sinovac inactivated SARS-CoV-2 vaccine. Four monoclonal antibodies namely 2B1B1, 4D3A3, 5G1F8, and 7C6F5 were produced using hybridoma technology. Titers of all four monoclonal antibodies in ELISA reached more than 1.28×10 6.0. Moreover, all monoclonal antibodies could react specifically with N protein expressed by transfection of pcDNA3.1-N into BHK-21 cells in IPMA and IFA. These results indicated that water-soluble N protein retained high immunogenicity and possessed the same epitopes as that of native N protein on virions. In addition, the preparation of water-soluble N protein and its monoclonal antibodies laid the basis for the development of immunoassays for COVID-19 detection.
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Authors and Affiliations

Y.B. Wang
1
S.W. Wang
2
Q.Y. Jin
3
L.P. Chen
4
F.Q. Zhang
1
J.J. Shi
1
Y. Yin
5
Z.X. Fan
1
X.Y. Liu
6
L.P. Wang
6
P. Li
6

  1. School of Public Health, Xinxiang Medical University, Xinxiang 453003, P.R. China
  2. School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang 453003, P.R. China
  3. Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, P.R. China
  4. Gushi County Center for Animal Disease Control and Prevention, Xinyang 465200, P.R. China
  5. Mingde College of Xinxiang Medical University, Xinxiang 453003, P.R. China
  6. School of Biological Engineering, Xinxiang University, Xinxiang 453003, P.R. China

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