In the present paper we show results of our research on the contents of selected heavy metals in the surface and subsurface water layers of the estuarine lake Gardno. Obtained results show that there are substantial differences between concentrations of lead, copper and zinc in surface microlayer and subsurface waters. We observed that the surface microlayer is capable of accumulating much higher amounts of these metals than the subsurface waters. Analysis of seasonal changes in heavy metal concentrations in surface microlayers and subsurface waters shows some periodicity of these changes.