Biological diversity within a mixture field allows for better use of habitat and agro-technical conditions by the mixtures, which can be seen by higher and more stable yields than varieties sown separately. Our studies were conducted in the growing seasons 2011/2012–2014/2015 as field experiments with four winter barley varieties (Bombaj, Gil, Gregor, Bażant) and three, two- and three-component mixtures (Bombaj/Gil, Bombaj/Gregor, Gil/Gregor/Bażant). Seven different chemical treatments with fungicides were applied. The aim of this study was to compare the different varieties of winter barley with their mixtures for resistance to powdery mildew infection. To achieve this aim the logistic model for the analysis of data was used. Of the varieties under consideration, the best and the most resistant variety was Gregor, while the weakest and the most susceptible to diseases (powdery mildew) was Gil. This variety was also significantly weaker than any of the other mixtures taken into account. Moreover, it was so weak that when it was included in mixtures with other varieties, it weakened these mixtures as well.