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Number of results: 6
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Abstract

Alginate – chitosan – alginate multilayer hydrogel encapsulation systems were investigated for

encapsulation of chondrocytes. Hydrogel is crosslinked due to ionic interaction between cationic

chitosan and anionic alginate, and additionally by calcium ions. Two types of chitosan with

molecular weight were investigated. Cells were encapsulated in two shape microcapsules, microbeads with diameter size 300 – 400 and 500 - 600 µm and fibres with diameter 500 - 600 µm. The

work provides a detailed examination of the impact of the microencapsulation process on the growth

of cells. The viability of chondrocytes can be influenced by the size of produced microcapsules,

while the shape of microcapsules has no important significance on cell viability. The applied

encapsulation methods do not contain harmful stages and create conducive conditions for cell

growth. A possible application area of the developed system is dressing and regeneration of

damaged joint cartilage.

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Authors and Affiliations

Iga Wasiak
Tomasz Ciach
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Abstract

A method of manufacturing hydrogel coatings designed to increase the hydrophilicity of polyurethanes (PU) is presented. Coatings were obtained from polyvinylpyrrolidone (PVP) by free radical polymerisation. The authors proposed a mechanism of a two-step grafting - crosslinking process and investigated the influence of reagent concentration on the coating’s physical properties - hydrogel ratio (HG) and equilibrium swelling ratio (ESR). A surface analysis of freeze-dried coatings using scanning electron microscopy (SEM) showed a highly porous structure. The presented technology can be used to produce biocompatible surfaces with limited protein and cell adhesive properties and can be applied in fabrication of number of biomedical devices, e.g. catheters, vascular grafts and heart prosthesis.

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Authors and Affiliations

Tomasz Ciach
Beata Butruk
Maciej Trzaskowski
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Abstract

Because of excellent properties, similar to natural bone minerals, and variety of possible biomedical applications, hydroxyapatite (HAp) is a valuable compound among the calcium phosphate salts. A number of synthesis routes for producing HAp powders have been reported. Despite this fact, it is important to develop new methods providing precise control over the reaction and having potential to scale-up. The main motivation for the current paper is a view of continuous synthesis methods toward medical application of produced hydroxyapatite, especially in the form of nanoparticles.

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Authors and Affiliations

Joanna Latocha
Michał Wojasiński
Paweł Sobieszuk
Tomasz Ciach
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Abstract

Every bone implant to work correctly after implantation needs to integrate with the surrounding bone. To enhance such a process, called osseointegration, various techniques of implant surface modification emerged. One of the approaches is based on the deposition of nano- and submicron materials on the implant surface. This paper presents a solution blow spinning process for producing poly-L-lactic acid (PLLA)/ceramic fibrous composites designed to be deposited directly onto orthopaedic implants prior to implantation to increase osseointegration. We produced plain PLLA fibrous materials for comparison, and fibrous composite materials with ��-tricalcium phosphate (��TCP), hydroxyapatite nanoparticles (nHAp) and hydroxyapatite nanoparticles modified with lecithin (nHAp-LE). We performed the structural analysis of produced materials with scanning electron microscopy, gravimetric determination of porosity, and water contact angle measurement. We also used infrared spectroscopy, Alizarin Red S staining, and cytotoxicity evaluation to conclude that PLLA/nHAp-LE composite material shows the most promising properties to be applied as surface modification of bone implants. To visualise fibrous composite deposition on implants, we used two models: titanium plate and stainlesssteel bolt. Thus, we showed that the solution blow spun materials can be used for surface modification of orthopaedic implants.
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Authors and Affiliations

Michał Wojasiński
1
Tomasz Ciach
1 2
ORCID: ORCID

  1. Warsaw University of Technology, Faculty of Chemical and Process Engineering, Warynskiego 1, 00-645 Warsaw, Poland
  2. Warsaw University of Technology, Centre for Advanced Materials and Technologies CEZAMAT, Poleczki 19, 02-822 Warsaw, Poland
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Abstract

Cell encapsulation seems to be a promising tool in tissue engineering. However, it has been shown to have several limitations in terms of long-term cell cultures due to an insufficient oxygen supply. In this study we propose the use of novel microcapsules designed for long-term cell culture consisting of an alginate shell and perfluorocarbon (PFC) core, which works as a synthetic oxygen carrier and reservoir. The influence of PFC presence in the culture as well as the size of structures on cell metabolism was evaluated during 21-day cultures in normoxia and hypoxia. We showed significant improvement in cell metabolism in groups where cells were encapsulated in hydrogel structures with a PFC core. The cells maintained a typical metabolism (oxidative phosphorylation) through all 21 days of the culture, overcoming the oxygen supply shortage even in large structures (diameter ¡ 1 mm). Applying PFC in alginate matrices can improve cell metabolism and adaptation in long-term cell cultures.
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Authors and Affiliations

Agata Stefanek
1
ORCID: ORCID
Aleksandra Kulikowska-Darłak
1
ORCID: ORCID
Karolina Bogaj
1
Aleksandra Nowak
1
Joanna Dembska
1
Tomasz Ciach
1
ORCID: ORCID

  1. Biomedical Engineering Laboratory, Faculty of Chemical and Process Engineering, Warsaw University of Technology, 00-645 Warsaw, Poland
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Abstract

Developing an effective and safe cancer therapy could significantly reduce the number of deaths and improve the quality of life of treated patients. Nowadays medicine has developed a wide range of anticancer chemotherapeutics but at the same time there is a lack of effective drug delivery methods. Therefore, the development of the targeted drug delivery system which will selectively release drug into the cancer cells is a key challenge of modern medicine.
The main aim of the presented research was to investigate the targeting effect of a drug delivery system based on the controlled release of dextran nanoparticles containing the anticancer drug – doxorubicin from the alginate microspheres coated with chitosan multilayers.
During the research the physicochemical properties of the alginate microspheres and its stability in the physiological environment were investigated. Moreover, the kinetics of the nanoparticles with doxorubicin release from the alginate microspheres covered with chitosan multilayers was characterized, depending on the thickness of the chitosan layer. Further, the cytotoxicity study of the alginate microspheres covered with chitosan multilayer and containing nanoparticles was performed to determine the therapeutic effect of the released nanoparticles with doxorubicin on the HeLa cells during the in vitro cell culture.
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Authors and Affiliations

Aleksandra Kulikowska-Darłak
1
ORCID: ORCID
Agata Stefanek
1
ORCID: ORCID
Iga Wasiak-Wojasińska
1
ORCID: ORCID
Paulina Wiechecka-Ożdżyńska
1
ORCID: ORCID
Tomasz Ciach
1
ORCID: ORCID

  1. Faculty of Chemical and Process Engineering, Warsaw University of Technology, Warynskiego 1 Street, 00-645 Warsaw, Poland

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