Abstract
The aim of this study was to analyze the chemiluminescence (CL) of peripheral blood in clinically healthy horses of different sexes and ages. The tests were carried out on 119 half- -breed horses, representing various forms of use (66 recreational horses and 53 sport horses). The test material was peripheral blood, which was collected under resting conditions, i.e. before physical activity related to the use of these animals. In the blood samples, spontaneous and stimulated CL with zymosan and phorbol myristate acetate were determined. It has been found that regular training effort increases the blood’s pro-oxidative potential, which was demonstrated by significantly higher (p<0.05) CL values in sport horses compared to recreational animals. Analysis of the results did not show any statistically significant correlation between sex or age of the horses with chemiluminescence values in peripheral blood. The result of the research suggests the need to optimize the results of blood CL measurements, taking into account the number of neutrophils and the concentration of hemoglobin in the blood of tested animals. Analysis of non-optimized blood CL results may lead to premature conclusions.
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Bibliography
Albright JM, Dunn RC, Shults JA, Boe DM, Afshar M, Kovacs EJ (2016) Advanced Age Alters Monocyte and Macrophage Responses. Antioxid Redox Signal 25: 805-815.
Bartoskova A, Ondrackova P, Leva L, Vitasek R, Novotny R, Janosovska M, Faldyna M (2014) The effects of in vitro exposure to proges-terone and estradiol-17β on the activity of canine neutrophils. Vet Med 59: 202-209.
Bedouhène S, Moulti-Mati F, Hurtado-Nedelec M, Dang PM, El-Benna J (2017) Luminol-amplified chemiluminescence detects mainly su-peroxide anion produced by human neutrophils. Am J Blood Res 7: 41-48.
Bereshchenko O, Bruscoli S, Riccardi C (2018) Glucocorticoids, Sex Hormones, and Immunity. Front Immunol 9: 1332.
Burlikowska K, Bogusławska-Tryk M, Szymeczko R, Piotrowska A (2015) Haematological and biochemical blood parameters in horses used for sport and recreation. J Cent Eur Agric 16: 370-382.
Caldefie-Chézet F, Walrand S, Moinard C, Tridon A, Chassagne J, Vasson MP (2002) Is the neutrophil reactive oxygen species production measured by luminol and lucigenin chemiluminescence intra or extracellular? Comparison with DCFH-DA flow cytometry and cytochrome C reduction. Clin Chim Acta 319: 9–17.
Cywińska A, Wyszyńska Z, Górecka R, Szarska E, Witkowski L, Dziekan P, Winnicka A, Schollenberger A (2010) The effect of the 162 km endurance rideon equine peripheral blood neutrophil and lymphocyte functions. Pol J Vet Sci 13: 279-285.
Cywińska A, Turło A, Witkowski L, Szarska E, Winnicka A (2014) Changes in blood cytokine concentrations in horses after long-distance endurance rides. Med Weter 70: 568-571.
Doucet DR, Bonitz RP, Feinman R, Colorado I, Ramanathan M, Feketeova E, Condon M, Machiedo GW, Hauser CJ, Xu DZ, Deitch EA (2010) Estrogenic hormone modulation abrogates changes in red blood cell deformability and neutrophil activation in trauma hemorrhagic shock. J Trauma 68: 35-41.
Escribano BM, Castejón FM, Vivo R, Santisteban R, Agűera EI, Rubio MD (2005) Effects of training on phagocytic and oxidative metabo-lism of peripheral neutrophils in horses exercised in the aerobic-anaerobic transition area. Vet Res Commun 29: 149-158.
Forslid J, Hed J (1982) In vitro effect of hydrocortisone on the attachment and ingestion phases of immunoglobulin G- and complement com-ponent 3b-mediated phagocytosis by human neutrophils. Infect Immun 38: 811-816.
Frape D (2010) Equine nutrition and feeding. 4th ed., Oxford, UK John Wiley & Sons Ltd, pp 90-179.
Hughes DL, Richards RS, Lexis LA (2018) Using chemiluminescence to determine whole blood antioxidant capacity in rheumatoid arthritis and Parkinson’s disease patients. Luminescence 33: 764-770.
Hyyppä S (2005) Endocrinal responses in exercising horses. Livestock Prod Sci 92: 113-121.
Iranifam M (2014) Analytical applications of chemiluminescence methods for cancer detection and therapy. TrAC Trends in Analytical Chem-istry 59: 156-183.
Jimenez AM, Navas MJ (2002) Chemiluminescence Methods (Present and Future). Grasas y Aceites 53: 64-75.
Klink M, Bednarska K, Blus E, Kiełbik M, Sulowska Z (2012) Seasonal changes in activities of human neutrophils in vitro. Inflamm Res 61: 11-16.
Koenig JB, Hart J, Harris DM, Cruz AM, Bienzle D (2009) Evaluation of endotoxin activity in blood measured via neutrophil chemilumines-cence in healthy horses and horses with colic. Am J Vet Res 70: 1183-1186.
Korhonen PA, Lilius EM, Hyyppä S, Räsänen LA, Pösö AR (2000) Production of reactive oxygen species in neutrophils after repeated bouts of exercise in Standardbred trotters. J Vet Med A Physiol Pathol Clin Med 47: 565-573.
Kraemer WJ, Ratamess NA, Hymer WC, Nindl BC, Fragala MS (2020) Growth Hormone(s), Testosterone, Insulin-Like Growth Factors, and Cortisol: Roles and Integration for Cellular Development and Growth with Exercise. Front Endocrinol (Lausanne) 11: 33.
Krumrych W, Wiśniewski E (2006) Influence of selected stimulators on chemiluminescence of peripheral blood neutrophils in horses. Med Weter 62: 204-206.
Krumrych W, Danek J (2012) Chemiluminescence of Peripheral Blood Neutrophils in Mares with Endometritis. Bull Vet Inst Pulawy 56: 51-56.
Krumrych W, Skórzewski R, Malinowski E (2013) The effect of storage on whole blood chemiluminescence measurement of equine neutro-phils. Luminescence 28: 327-331.
Krumrych W, Gołda R, Gołyński M, Markiewicz H, Buzała M (2018) Effect of physical exercise on cortisol concentration and neutrophil oxygen metabolism in peripheral blood of horses. Ann Anim Sci 18: 53-68.
Lewkowicz P, Lauk-Puchala B, Banasik M, Gorańska N, Tchórzewski H (1999) A standarisation attempt of a measurment method of the whole blood chemiluminescency as a means to estimate human neutrophiles functions. Diagn Lab 35: 497-510. (In Polish with English ab-stract).
Li Z, Jiang H, Xie W, Zhang Z, Smrcka AV, Wu D (2000) Role of PLC-beta2 and – beta3 and PI3Kgamma in chemoattractant – mediated signal transduction. Science 287: 1046-1049.
Liburt NR, Adams AA, Betancourt A, Horohov DW, McKeever KH (2010) Exercise-induced increases in inflammatory cytokines in muscle and blood of horses. Equine Vet J Suppl 38: 280-288.
Marin DP, Bolin AP, de Cássia Santos Macedo R, Curi R, Otton R (2010) Testosterone suppresses oxidative stress in human neutrophils. Cell Biochem Funct 28: 394-402.
Martin EM, Till RL, Sheats MK, Jones SL (2017) Misoprostol Inhibits Equine Neutrophil Adhesion, Migration, and Respiratory Burst in an In Vitro Model of Inflammation. Front Vet Sci 4: 159.
McTaggart C, Yovich JV, Penhale J, Raidal SL (2001) A comparison of foal and adult horse neutrophil function using flow cytometric tech-niques. Res Vet Sci 71: 73-79.
Nelson RJ (2004) Seasonal immune function and sickness responses. Trends Immunol 25: 187-192.
Nieman DC, Wentz LM (2019) The compelling link between physical activity and the body’s defense system. J Sport Health Sci 8: 201-217.
Okunnu BM, Berg RE (2019) Neutrophils Are More Effective than Monocytes at Phagosomal Containment and Killing of Listeria mono-cytogenes. Immunohorizons 3: 573-584.
Ortega E (2003) Neuroendocrine mediators in the modulation of phagocytosis by exercise: physiological implications. Exerc Immunol Rev 9: 70-93.
Papp Z, Smits JE (2007) Validation and novel applications of the whole-blood chemiluminescence assay of innate immune function in wild vertebrates and domestic chickens. J Wildl Dis 43: 623-634.
Primary immunodeficiency diseases. Report of WHO Scientific Group (1997) Clin Exp Immunol 109 Suppl 1: 1-28.
Raidal SL, Love DN, Bailey GD, Rose RJ (2000) Effect of single bouts of moderate and high intensity exercise and training on equine pe-ripheral blood neutrophil function. Res Vet Sci 68: 141-146.
Robson PJ, Alston TD, Myburgh KH (2003) Prolonged suppression of the innate immune system in the horse following an 80 km endurance race. Equine Vet J 35: 133-137.
Shaw AC, Joshi S, Greenwood H, Panda A, Lord JM (2010) Aging of the innate immune system. Curr Opin Immunol 22: 507-513.
Siddiqi M, Garcia ZC, Stein DS, Denny TN, Spolarics Z (2001) Relationship between oxidative burst activity and CD11b expression in neu-trophils and monocytes from healthy individuals: effects of race and gender. Cytometry 46: 243-246.
Simpson RJ, Kunz H, Agha N, Graff R (2015) Exercise and the Regulation of Immune Functions. Prog Mol Biol Transl Sci 135: 355-380.
Szuster-Ciesielska A, Kandefer-Szerszeń M (2002) The influence of sex and age on serum catalase, peroxidase, superoxide dismutase activity and production of O-2 and H2O2 by human blood neutrophils. Ann UMCS sect C Lublin – Polonia 57: 1-12.
Terra R, Gonçalves da Silva SA, Salerno Pinto V, Dutra PM (2012) Effect of exercise on the immune system: response, adaptation and cell signaling. Rev Bras Med Esporte 18: 208-214.
Walton RH, Lawson CA (2021) Equine Hematology. In: Walton RM, Cowell RL, Valenciano AC (eds) Equine Hematology, Cytology, and Clinical Chemistry. 2nd ed., Hoboken, USA, John Wiley & Sons, Inc, pp 9-26.
Wenisch C, Patruta S, Daxböck F, Krause R, Hörl W (2000) Effect of age on human neutrophil function. J Leukoc Biol 67: 40-45.
Yellon SM, Fagoaga OR, Nehlsen-Cannarella SL (1999) Influence of photoperiod on immune cell functions in the male Siberian hamster. Am J Physiol 276: R97-R102.
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