Highly active antagonistic actinomycete Streptomyces griseoviridis and entomopathogenic fungus Beauveria bassiana were applied to the soil separately and together (in association) in the laboratory experiments. We assessed survival rate, insecticidal and fungistatic activity of these strains. We also tested the influence of synthetic insecticide Regent 25® (fipronil 25g/l) on investigated parameters. Additionally, insecticidal activity of both strains was compared with insecticidal activity of Regent. It was shown that both strains, especially S. griseoviridis, good survived in soil. Population density of S. griseoviridis in the association with B. bassiana increased 2–3 times compared to initial density. Regent considerably reduced population density of S. griseoviridis and B. bassiana. Insecticidal efficiency of S. griseoviridis was comparable with the effect of synthetic incecticide Regent and reached 89.2% and 86.8% respectively. Fungistatic activity towards Fusarium oxysporum showed only S. griseoviridis and it was observed that this activity decreased in time course.