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Number of results: 5
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Modal parameter identification of cable stayed bridge based on Exploratory Data Analysis

I. Khan D. Shan Q. Li
Archives of Civil Engineering | 2015 | No 2 | 3-22
Keywords EDA DATA-SSI outlier analysis stabilization diagram
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Abstract

In order to identify the modal parameters of civil structures it is vital to distinguish the defective data from that of appropriate and accurate data. The defects in data may be due to various reasons like defects in the data collection, malfunctioning of sensors, etc. For this purpose Exploratory Data Analysis (EDA) was engaged toenvisage the distribution of sensor’s data and to detect the malfunctioning with in the sensors. Then outlier analysis was performed to remove those data points which may disrupt the accurate data analysis. Then Data Driven Stochastic Sub-space Identification (DATA-SSI) was engaged to perform the modal parameter identification. In the end to validate the accuracy of the proposed method stabilization diagrams were plotted. Sutong Bridge, one of the largest span cable stayed bridge was used as a case study and the suggested technique was employed. The results obtained after employing the above mentioned techniques are very valuable, accurate and effective.

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Authors and Affiliations

I. Khan
D. Shan
Q. Li

GP5 Protein-based ELISA for the Detection of PRRSV Antibodies

J. Yang Y. Wang J. Guo S. Qiao Q. Li Q. Jin G. Zhang
Polish Journal of Veterinary Sciences | 2016 | No 3 | DOI: 10.1515/pjvs-2016-0062
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Authors and Affiliations

J. Yang
Y. Wang
J. Guo
S. Qiao
Q. Li
Q. Jin
G. Zhang

Triplex qRT-PCR with specific probe for synchronously detecting Bovine parvovirus, bovine coronavirus, bovine parainfluenza virus and its applications

J. Geng Y. Niu L. Wei Q. Li Z. Gong S. Wei
Polish Journal of Veterinary Sciences | 2020 | vol. 23 | No 4 | 481-489 | DOI: 10.24425/pjvs.2020.134696
Keywords bovine parvovirus bovine coronavirus bovine parainfluenza virus qRT-PCR specificity sensitivity
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Abstract

Bovine parvovirus (BPV), bovine coronavirus (BCoV) and bovine parainfluenza virus (BPIV) are common etiologies causing gastrointestinal and respiratory diseases in dairy herds. However, there are few reports on the synchronous detection of BPV, BCoV and BPIV. The present article aimed to develop a quick and accurate RT-PCR assay to synchronously detect BPV, BCoV and BPIV based on their specific probes. One pair universal primers, one pair specific primers and one specific probe was designed and synthesized. After the concentrations of primer and probe and annealing temperature were strictly optimized, the specificity, sensitivity and repeatability of the established triplex probe qRT-PCR were evaluated, respectively. The results showed the recombinant plasmids of pMD18-T-BPV, pMD18-T-BCoV and pMD18-T-BPIV were 554bp, 699bp and 704bp, respectively. The optimal annealing temperature was set at 45.0°C for triplex qRT-PCR. The triplex probe qRT-PCR can only synchronously detect BPV, BCoV and BPIV. Detection sensitivities were 2.0×102, 2.0×102 and 2.0×101 copies/μL for BPV, BCoV and BPIV, being 1000-fold greater than that in the conventional PCR. Detection of clinical samples demon- strated that triplex probe qRT-PCR had a higher sensitivity and specificity. The intra-assay and inter-assay coefficient of variation were lower than 2.0%. Clinical specimens verified that the triplex qRT-PCR had a higher sensitivity and specificity than universal PCR. In conclusion, this triplex probe qRT-PCR could detect only BPV, BCoV and BPIV. Minimum detection limits were 2.0×102 copies/μL for BPV and BCoV, and 2.0×101 copies/μL for BPIV. The sensitivity of this triplex probe qRT-PCR was 1000-fold greater than that in the conventional PCR. The newly qRT-PCR could be used to monitor or differentially diagnose virus infection.

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Authors and Affiliations

J. Geng
Y. Niu
L. Wei
Q. Li
Z. Gong
S. Wei

Development of a blocking immunoperoxidase monolayer assay for differentiation between pseudorabies virus-infected and vaccinated animals

Y.B. Wang Y.H. Li Q.M. Li W.T. Xie C.L. Guo J.Q. Guo R.G. Deng G.P. Zhang
Polish Journal of Veterinary Sciences | 2019 | vol.22 | No 4 | 717-723 | DOI: 10.24425/pjvs.2019.129985
Keywords pseudorabies virus variant strains anti-pseudorabies virus monoclonal antibody blocking immunoperoxidase monolayer assay differentiation between pseudorabies virus-infected and vaccinated animals
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Abstract

Pseudorabies (PR) outbreaks have devastated many swine farms in several parts of China since late 2011. The outbreak-associated pseudorabies virus (PRV) variant strains exhibited some typical amino acid changes in glycoprotein E (gE), a diagnostic antigen used for discriminating between PRV-infected and vaccinated animals (DIVA). To counteract the potential impact of epitope variations on current serological diagnostics of PRV, we produced monoclonal antibodies (mAbs) against gE protein of one representative PRV variant strain and developed a blocking immunoperoxidase monolayer assay (b-IPMA) for DIVA. The b-IPMA was based on the inhibition of binding between PRV-infected cells and mAb by PRV-specific antibodies present in clinical swine sera and was validated by comparison with a commercial PRV gpI Antibody Test Kit (IDEXX Laboratories, USA). The diagnostic sensitivity, diagnostic specificity and agreement were determined to be 99.25%, 98.18% and 99.02% respectively upon testing 509 serum samples. b-IPMA detected only PRV-specific antibodies and showed no cross- -reactivity with antibodies elicited by gE-deleted vaccine or other common swine pathogens. Thus, b-IPMA has the potential to be used for high-throughput screening of PRV-infected animals in veterinary clinics.

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Authors and Affiliations

Y.B. Wang
Y.H. Li
Q.M. Li
W.T. Xie
C.L. Guo
J.Q. Guo
R.G. Deng
G.P. Zhang

ABHD2 deficiency aggravates ovalbumin-induced airway remodeling through the PI3K/Akt pathway in an animal model of chronic asthma

L. Qiang X. Li Q. Li H. Bo Y. Liu M. Lv X. Chen H. Ju X. Sang Z. Li S. Jin
Polish Journal of Veterinary Sciences | 2023 | vol. 26 | No 4 | 635-646 | DOI: 10.24425/pjvs.2023.148283
Keywords Abhd2 airway remodeling, inflammation PI3K/Akt signalling pathway
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Abstract

Airway remodeling is a major pathological characteristic of chronic obstructive pulmonary disease (COPD). This study aimed to investigate the effect of Abhd2 deficiency on ovalbumin (OVA)-induced airway remodeling and inflammation in vivo. Abhd2-deficient mice were used to establish an OVA-induced asthma model. Lung tissues were analyzed using hematoxylin and eosin (HE) staining, Masson staining, immunohistochemistry, quantitative reverse transcription- polymerase chain reaction (qRT-PCR), and western blotting were used to determine the role of Abhd2 in the regulation of OVA-induced airway remodeling and inflammation. Our findings revealed that the RNA expression of inflammatory factors, including IL-1β, IL-6, IL-4, and IL-13, was significantly increased in OVA-induced Abhd2 Gt/Gt asthmatic mice. The expression of IFN-γ was decreased significantly in OVA-induced Abhd2 Gt/Gt asthmatic mice. The protein expression of airway remodeling factors, including α-SMA, type I collagen, and Ki67, was also increased in OVA-induced Abhd2 Gt/Gt asthmatic mice compared to that in OVA-induced wild-type (WT) mice. Additionally, Abhd2 deficiency promoted the expression of p-Akt in tissues of the asthma model. These results suggest that Abhd2 deficiency exacerbates airway remodeling and inflammation through the PI3K/Akt pathway in chronic asthma.
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Authors and Affiliations

L. Qiang
1
X. Li
1
Q. Li
2
H. Bo
3
Y. Liu
1
M. Lv
1
X. Chen
1
H. Ju
1
X. Sang
1
Z. Li
4
S. Jin
1
  1. Department of Respiratory Medicine, Fourth Affiliated Hospital, Harbin Medical University, 37# Yiyuan Street, Harbin 150001, Heilongjiang, China
  2. Department of pulmonary diseases, Heilongjiang Academy of Traditional Chinese Medicine, 33# Xidazhi Street, Harbin 150036, Heilongjiang, China
  3. Department of Intensive Care Unit, Fourth Affiliated Hospital, Harbin Medical University, 37# Yiyuan Street, Harbin 150001, Heilongjiang, China
  4. University of Tokyo, 3-8-1# Bunkyo ku, Tokyo 1130033, Tokyo, Japan

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