The effects of application of an artificial honeydew mixture of glucose, fructose and trehalose (GFT), honey and Bemisia tabaci nymph-extract as kairomonal sources in enhancing the foraging efficiency and performance of Eretmocerus sp. near furuhashii on cucumber plants were studied. Experiments were conducted in small greenhouses (4×3×3 m) using life table methods. Life table data indicated that the total mortality in B. tabaci immature cohorts in all treatments was in the order of fourth instar > first instar > second = third > egg > pupa cohorts. The tested kairomonal materials had a significant effect on the rate of parasitism (p > 0.0415) with 13.23, 9.04 and 10.54% higher than that of control in artificial honeydew of GFT, nymph-extract and honey treatments, respectively. B. tabaci egg/adult survival ratio was also significantly affected (p > 0.0001) by the tested kairomonal sources being lowest (22.91%) in nymph-extract treatment. Moreover, the tested kairomonal materials arrested significantly more parasitoids to colonize the treated plants comparing to control. Apparently, the tested materials were significantly effective in attracting the parasitoids up to 3 days after applications then significant difference was not found between treatments.
The full-length cDNA of LeTIR1 gene was isolated from tomato with EST-based in silico cloning followed by RACE amplification. LeTIR1 contained an open reading frame (ORF) 1872 bp long, encoding 624 amino acid residues. The predicted protein LeTIR1 had one F-box motif and eleven leucine-rich repeats (LRRs), all of which are highly conserved in TIR1 proteins of other plant species. Phylogenetic analysis showed that the LeTIR1 protein shared high similarity with other known TIR1 proteins. Both sequence and phylogenetic analysis suggested that LeTIR1 is a TIR1 homologue and encodes an F-box protein in tomato. Semi-quantitative RT-PCR indicated that LeTIR1 was expressed constitutively in all organs tested, with higher expression in stem than root, leaf, flower and fruit. Its expression level was positively correlated with the auxin distribution in stem or axillary shoot, and was induced by spraying exogenous IAA.