Search results

Filters

  • Journals
  • Authors
  • Keywords
  • Date
  • Type

Search results

Number of results: 2
items per page: 25 50 75
Sort by:
Download PDF Download RIS Download Bibtex

Abstract

Onion yellow dwarf virus (OYDV), an aphid-borne potyvirus is one of the major viral pathogens of garlic causing significant yield losses worldwide. It is found almost everywhere in the world where Allium species is grown. The aim of this study was to test the presence of OYDV infection in garlic from Ethiopia. The presence of the virus was tested by Reverse transcription polymerase chain reaction (RT-PCR). The direct sequencing of the PCR product produced a sequence of 296 bp. Sequence analysis showed 89.27% sequence homology with an isolate from Australia (HQ258894) and 89.29% with an isolate from Spain (JX429964). A phylogenetic tree constructed with MEGA 7.0 revealed high levels of homology with various isolates of OYDV from all over the world and thus further confirmed the identity of the virus.

Go to article

Authors and Affiliations

Yohanis Kebede
Jyoti Singh
Shahana Majumder
ORCID: ORCID
Download PDF Download RIS Download Bibtex

Abstract

Banana is the major fruit crop produced in Ethiopia. Since Cucumber mosaic virus (CMV) is one of the most devastating plant viruses infecting banana, the present study was undertaken to survey and identify CMV strains infecting banana plants in Ethiopia. Dot immune-binding assay (DIBA) and reverse transcription-polymerase chain reaction (RT-PCR) revealed the presence of CMV in all of the symptomatic samples tested. The results of sequence and phylogenetic analysis revealed that the isolate under study was a CMV isolate from the IB subgroup. Multiple sequence alignment revealed a three nucleotide sequence variation that could be used to distinguish CMV subgroups. Selection pressure analysis showed the CMV-RNA1 region undergoing positive selection pressure. Tajima`s test of neutrality revealed a positive value of 0.86468 indicating CMV population contraction. To the best of our knowledge, this is the first report and molecular characterization of CMV IB subgroup isolate infecting banana plants in Ethiopia.
Go to article

Bibliography


Basavaraj S., Rangaswamy K.T., Bhagyashree M. 2017. Molecular characterization of CMV infecting banana from Karnataka based on complete coat protein gene sequence. International Journal of Current Microbiology and Applied Sciences 6 (11): 3758–3763. DOI: https://doi.org/10.20546/ ijcmas.2017.611.440
Bujarski J.J. 2021. Bromoviruses (Bromoviridae). p. 260–267. “Encyclopedia of Virology” 4th ed. (D.H. Bamford, M. Zuckerman, eds.). Academic Press, Oxford. DOI: https://doi.org/10.1016/B978-0-12-809633-8.21563-X
Canto T., Palukaitis P. 2001. A Cucumber mosaic virus (CMV) RNA 1 transgene mediates suppression of the homologous viral RNA 1 constitutively and prevents CMV entry into the phloem. Journal of Virology 75 (19): 9114–9120. DOI: https://doi.org/10.1128/JVI.75.19.9114-9120.2001
Chou C.N., Chen C.E., Wu M.L., Su H.J., Yeh H.H. 2009. Biological and molecular characterization of Taiwanese isolates of Cucumber mosaic virus associated with banana mosaic disease. Journal of Phytopathology 157 (2): 85–93. DOI: https://doi.org/10.1111/j.1439-0434.2008.01455.x
Domingo E., Holland J.J. 1994. Mutation rates and rapid evolution of RNA viruses. p. 161–184. In: “The Evolutionary Biology of Viruses” (S.S. Morse, ed.). Raven Press, New York.
Doolittle S.P. 1916. A new infectious mosaic disease of cucumber. Phytopathology 6: 145–147.
Garcia-Arenal F., Palukaitis P. 2008. Cucumber mosaic virus. Desk encyclopedia of plant and fungal virology. p. 171–176. In: “Desk Encyclopedia of Plant and Fungal Virology” (B.W.J. Mahy, M.H.V. Van Regenmortel, eds.). Academic Press-Elsevier, Oxford, United Kingdom.
Garcia-Arenal F., Fraile A. 2011. Population dynamics and genetics of plant infection by viruses. p. 263–281. In: ”Recent Advances in Plant Virology” (C. Caranta, M.A. Aranda, M. Tepfer, J.J. Lopez-Moye, eds.). Caister Academic Press, Norfolk, United Kingdom.
Gorbalenya A.E., Koonin E.V., Donchenko A.P., Blinov V.M. 1989. Two related superfamilies of putative helicases involved in replication, recombination, repair and expression of DNA and RNA genomes. Nucleic Acids Research 17 (12): 4713–4730. DOI: https://doi.org/10.1093/nar/17.12.4713
Hampton R.O., Francki R.I.B. 1992. RNA-1 dependent seed transmissibility of cucumber mosaic virus in Phaseolus vulgaris. Phytopathology 82 (2): 127–130.
Kang W.H., Seo J.K., Chung B.N., Kim K.H., Kang B.C. 2012. Helicase domain encoded by Cucumber mosaic virus RNA1 determines systemic infection of Cmr1 in pepper. PLoS One 7 (8): e43136. DOI: https://doi.org/10.1371/journal.pone.0043136
Kebede Y., Majumder S. 2020. Molecular detection and first report of Cucumber mosaic virus infecting ‘Cavendish’ banana plants in Ethiopia. Journal of Plant Disease and Protection 127: 417–420. DOI: https://doi.org/10.1007/s41348-020-00315-z
Khan S., Jan A.T., Aquil B., Mohd Q., Haq R. 2011. Coat protein gene based on characterization of cucumber mosaic virus isolates infecting banana in India. Journal of Phytology 3: 94–101.
Kim M.K., Seo J.K., Kwak H.R., Kim J.S., Kim K.H., Cha B.J., Choi H.S. 2014. Molecular genetic analysis of Cucumber mosaic virus populations infecting pepper suggests unique patterns of evolution in Korea. Phytopathology 104 (9): 993–1000. DOI: https://doi.org/10.1094/PHYTO-10-13-0275-R
Kumar A., Hanson J., Jones C.S., Assefa Y., Mulatu F. 2020. Screening and characterization of virus causing yellow leaf disease of Tephrosia in Ethiopia. Australasian Plant Pathology 49: 447–450. DOI: https://doi.org/10.1007/s13313-020-00717-5
Kumari R., Bhardwaj P., Singh L., Zaidi A.A., Hallan V. 2013. Biological and molecular characterization of Cucumber mosaic virus subgroup II isolate causing severe mosaic in cucumber. Indian Journal of Virology 24 (1): 27–34. DOI: 10.1007/s13337-012-0125-9
Lakshman D.K., Gonsalves D. 1985. Genetic analyses of two large-lesion isolates of Cucumber mosaic virus. Phytopathology 75 (7): 758–762.
Leach J.E., Vera Cruz C.M., Bai J., Leung H. 2001. Pathogen fitness penalty as a predictor of durability of disease resistance genes. Annual Review of Phytopathology 39 (1): 187–224. DOI: https://doi.org/10.1146/annurev.phyto.39.1.187
Liu Y.Y., Yu S.L., Lan Y.F., Zhang C.L., Hou S.S., Li X.D., Zhu X.P. 2009. Molecular variability of five Cucumber mosaic virus isolates from China. Acta Virologica 53 (2): 89–97. DOI: 10.4149/av_2009_02_89
Martin D.P., Murrell B., Golden M., Khoosal A., Muhire B. 2015. RDP4: detection and analysis of recombination patterns in virus genomes. Virus Evollution 1 (1). Available on: https:// doi.org/10.1093/ve/vev003 [Accessed: 10 June 2020]
Nouri S., Arevalo R., Falk B.W., Groves R.L. 2014. Genetic structure and molecular variability of Cucumber mosaic virus isolates in the United States. PLoS One 9 (5): e96582. DOI: https://doi.org/10.1371/journal.pone.0096582
Palukaitis P., Garcia-Arenal F. 2003. Cucumoviruses. Advances in Virus Research 62: 241–323. DOI: 10.1016/s0065-3527-(03)62005-1
Palukaitis P., Roossinck M.J., Dietzgen R.G., Francki R.I.B. 1992. Cucumber mosaic virus. Advances in Virus Research 41: 281–348. DOI: http://dx.doi.org/10.1016/s0065-3527-(08)60039-1
Prince W.C. 1934. Isolation and study of some yellow strains of Cucumber mosaic virus. Phytopathology 24: 743–761.
Roossinck M.J., Zhang L., Hellwald K.H. 1999. Rearrangements in the 5’ nontranslated region and phylogenetic analyses of cucumber mosaic virus RNA 3 indicate radial evolution of three subgroups. Journal of Virology 73 (8): 6752–6758. DOI: https://doi.org/10.1128/jvi.73.8.6752-6758.1999
Roossinck M.J. 1997. Mechanisms of plant virus evolution. Annual Review of Phytopathology 35 (1): 191–209. DOI: https://doi.org/10.1146/annurev.phyto.35.1.191
Roossinck M.J. 2001. Cucumber mosaic virus, a model for RNA virus evolution. Molecular Plant Pathology 2 (2): 59–63. DOI: https://doi.org/10.1046/j.1364-3703.2001.00058.x
Roossinck M.J., Palukaitis P. 1990. Rapid induction and severity of symptoms in zucchini squash (Cucurbita pepo) map to RNA 1 of Cucumber mosaic virus. Molecular Plant-Microbe Interactions 3 (1): 188–192. DOI: 10.1094/MPMI-3-188
Rozanov M.N., Koonin E.V., Gorbalenya A.E. 1992. Conservation of the putative methyltransferase domain: a hallmark of the ‘Sindbis-like’supergroup of positive-strand RNA viruses. Journal of General Virology 73 (8): 2129–2134. DOI: https://doi.org/10.1099/0022-1317-73-8-2129
Rozas J., Ferrer-Mata A., Sanchez-DelBarrio J.C., Guirao- -Rico S., Librado P., Ramos-Onsins SE, Sanchez-Gracia A. 2017. DnaSP 6: DNA sequence polymorphism analysis of large data sets. Molecular Biology and Evolution 34 (12): 3299–3302. DOI: https://doi.org/10.1093/molbev/msx248
Rybicki E.P. 2015. A top ten list for economically important plant viruses. Archives of Virology 160 (1): 17–20. DOI: 10.1007/s00705-014-2295-9
Seo J.K., Kwon S.J., Choi H.S., Kim K.H. 2009. Evidence for alternate states of Cucumber mosaic virus replicase assembly in positive-and negative-strand RNA synthesis. Virology 383 (2): 248–260. DOI: https://doi.org/10.1016/j.virol.2008.10.033
Simon A.E., Bujarski J.J. 1994. RNA-RNA recombination and evolution in virus-infected plants. Annual Review of Phytopathology 32 (1): 337–362.
Zitter T.A., Murphy J.F. 2009. The plant health instructor: Cucumber mosaic virus. American Phytopathological Society. DOI: 10.1094/PHI-I-2009-0518-01 [Accessed: 25 July 2020]
Go to article

Authors and Affiliations

Yohanis Kebede
1
Shahana Majumder
2
ORCID: ORCID

  1. Department of Biotechnology, Sharda University, Greater Noida, Uttar Pradesh, India
  2. Department of Botany, Mahatma Gandhi Central University, Bihar, India

This page uses 'cookies'. Learn more