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Abstract

The aim of this study was to analyze cases of granulocytic anaplosmosis diagnosed in 53 hunting dogs in Poland. Medical records of dogs naturally infected with Anaplasma phagocytophilum were retrospectively evaluated with regard to clinical signs and laboratory abnormalities at the time of presentation, therapy and course of disease. The most common clinical signs in A. phagocytophilum-positive dogs included in the study were lethargy (100%), inappetence (94%) and fever (92.5%). Thrombocytopenia was the most common laboratory abnormality (100%), followed by a drop in haematocrit level (79.3%) and increased AST activity (75.5%).
Of the 53 infected dogs, 51 (96%) recovered and two dogs (with neurological symptoms) died.
Analysis of these cases indicates that A. phagocytophilum infection must be considered in differential diagnosis in dogs living in Poland, especially in hunting dogs with thrombocyto- penia and Ixodes ricinus tick invasions.
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Authors and Affiliations

O. Teodorowski
1
S. Winiarczyk
2
P. Debiak
3
M. Skrzypczak
4
Ł. Mazurek
2
Ł. Adaszek
2

  1. “Teodorowscy” Veterinary Clinic in Mikołów, Poland
  2. Department of Epizootiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Głęboka 30, 20-612 Lublin, Poland
  3. Department and Clinic of Animal Surgery, Laboratory of Radiology and Ultrasonography, University of Life Sciences, Głęboka 30, 20-612 Lublin, Poland
  4. Second Department of Gynecology, Prof. F. Skubiszewski University School of Medicine, Lublin, Poland
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Abstract

The aim of the study was to compare the serum protein profile of Bernese Mountain Dogs (BMDs) reacting positive for Bb in snap testing with the serum protein profile of dogs of other breeds (healthy and with clinical borreliosis) using the MALDI time-of-flight (MALDI-TOF) technique. The observations included five groups of dogs. BMDs reacting positively to Bb in snap serological testing and showing symptoms of borreliosis (group 1), BMDs for which no borreliosis symptoms were determined but with seropositivity for Bb determined with snap serological tests (group 2), clinically healthy BMDs with no antibodies for Bb found in the serum (group 3), five dogs of different breeds, reacting positively in serological testing, in which borreliosis symptoms were observed (group 4), clinically healthy dogs of different breeds with negative reaction in tests towards Bb (group 5). A proteomic analysis demonstrated the presence of five identical protein fractions among all five groups. An additional two protein fractions of approximately 7.630 and 15.260 kDa were found in all the serum samples obtained from the dogs positive for borrelia in a snap test, both in those exhibiting symptoms of borreliosis, and seropositive BMDs not presenting symptoms of the disease. These two additional protein fractions may be used to differentiate between seropositive and seronegative B. burgdorferi dogs and may be considered a seropositivity marker, however, it cannot be used to differentiate between animals with the clinical form of the disease and those that are only seropositive.
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Bibliography

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Authors and Affiliations

M. Pisarek
1
M. Kalinowski
1
M. Skrzypczak
2
Ł. Mazurek
1
K. Michalak
1
D. Pietras-Ożga
1
B. Dokuzeylü
3
S. Winiarczyk
1
Ł. Adaszek
1

  1. Department of Epizoology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine of the University of Life Sciences in Lublin, ul. Głęboka 30, 20-612 Lublin, Poland
  2. Second Department of Gynecology, Medical University of Lublin, 20-954 Lublin, Poland
  3. Department of Internal Medicine, Veterinary Faculty, Istanbul University-Cerrahpasa, 34320 Avcilar Campus, Avcilar, Istanbul, Turkey

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