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Abstract

B a c k g r o u n d: The aim of this study was to determine the effect of sesquiterpene lactone parthenolide on the cytotoxic and pro-oxidative effects of etoposide in HL-60 cells.
M e t h o d s: Cytotoxic effects were determined by incubation of HL-60 cells with various concentrations of examined compounds and combinations thereof, which were then stained with propidium iodide and analyzed using a flow cytometer. To determine the role of oxidative stress in the action of the compounds, co-incubation with N-acetyl-l-cysteine (NAC) and parthenolide and/or etoposide was used and the level of reduced glutathione (GSH) was detected.
R e s u l t s: Parthenolide significantly enhanced the cytotoxic and pro-apoptotic effects of etoposide. However, in most cases of the combinations of parthenolide and etoposide, their effect was antagonistic, as confirmed by an analysis using the CalcuSyn program. The examined compounds significantly reduced the level of GSH in HL-60 cells. Combination of etoposide at a concentration of 1.2 μM and parthenolide also significantly reduced GSH level. However, in the case of a combination of etoposide at a concentration of 2.5 μM with parthenolide, a significant increase in the level of GSH was obtained compared to compounds acting alone. This last observation seems to confirm the antagonism between the compounds tested.
C o n c l u s i o n s: Parthenolide did not limit the cytotoxic effect of etoposide in HL-60 cells even in the case of antagonistic interaction. If parthenolide does increase GSH levels in combination with etoposide in the normal hematopoietic cells, it could protect them against the pro-oxidative effects of this anti-cancer drug.
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Bibliography

1. Bell J.A., Galaznik A., Huelin R., Stokes M., Guo Y., Fram R.J., Faller D.V.: Effectiveness and safety of therapeutic regimens for elderly patients with acute myeloid leukemia: a systematic literature review. Clin Lymphoma Myeloma Leuk. 2018; 18: e303–e314.
2. Hackl H., Astanina K., Wieser R.: Molecular and genetic alterations associated with therapy resistance and relapse of acute myeloid leukemia. J Hematol Oncol. 2017; 20: 51.
3. Foran J.M.: Do cytogenetics affect the post-remission strategy for older patients with AML in CR1? Best Pract Res Clin Haematol. 2017; 30: 306–311.
4. Yunos N.M., Beale P., Yu J.Q., Huq F.: Synergism from the combination of oxaliplatin with selected phytochemicals in human ovarian cancer cel lines. Anticancer Res. 2011; 31: 4283–4290.
5. Shah K., Mirza S., Desai U., Jain N., Rawal R.: Synergism of curcumin and cytarabine in the down regulation of multi-drug resistance genes in acute myeloid leukemia. Anticancer Agents Med Chem. 2016; 16: 128–135.
6. Banudevi S., Swaminathan S., Maheswari K.U.: Pleiotropic role of dietary phytochemicals in cancer: emerging perspectives for combinational therapy. Nutr Cancer. 2015; 67: 1021–1048.
7. Pei S., Minhajuddin M., D’Alessandro A., Nemkov T., Stevens B.M., Adane B., Khan N., Hagen F.K., Yadav V.K., De S., Ashton J.M., Hansen K.C., Gutman J.A., Pollyea D.A., Crooks P.A., Smith C., Jordan C.T.: Rational design of a parthenolide-based drug regimen that selectively eradicates acute myelogenous leukemia stem cells. J Biol Chem. 2016; 291: 21984–22000.
8. Guzman M.L., Rossi R.M., Karnischky L., Li X., Peterson D.R., Howard D.S., Jordan C.T.: The sesquiterpene lactone parthenolide induces apoptosis of human acute myelogenous leukemia stem and progenitor cells. Blood. 2005; 105: 4163–4169.
9. Papiez M.A., Baran J., Bukowska-Straková K., Wiczkowski W.: Antileukemic action of (-)-epicatechin in the spleen of rats with acute myeloid leukemia. Food Chem Toxicol. 2010; 48: 3391–3397.
10. Papież M.A.: The influence of curcumin and (-)-epicatechin on the genotoxicity and myelosuppression induced by etoposide in bone marrow cells of male rats. Drug Chem Toxicol. 2013; 36: 93–101.
11. Siveen K.S., Uddin S., Mohammad R.M.: Targeting acute myeloid leukemia stem cel signaling by natural products. Mol Cancer. 2017; 16: 1–12.
12. Curry E.A., Murry D.J., Yoder C., Fife K., Armstrong V., Nakshatri H., O’Connell M., Sweeney C.J.: Phase I dose escalation trial of feverfew with standardized doses of parthenolide in patients with cancer. Invest New Drugs. 2004; 22: 299–305.
13. Knight D.W.: Feverfew: chemistry and biological activity. Nat Prod Rep. 1995; 12: 271–276.
14. Ordóñez P.E., Sharma K.K., Bystrom L.M., Alas M.A., Enriquez R.G., Malagón O., Jones D.E., Guzman M.L., Compadre C.M.: Dehydroleucodine, a Sesquiterpene Lactone from Gynoxys verrucosa, Demonstrates Cytotoxic Activity against Human Leukemia Cells. J Nat Prod. 2016; 79: 691–696.
15. Merfort I.: Perspectives on sesquiterpene lactones in inflammation and cancer. Curr Drug Targets. 2011; 12: 1560–1573.
16. Li C., Jones A.X., Lei X.: Natural product reports synthesis and mode of action of oligomeric sesquiterpene lactones. Nat Prod Rep. 2015; 1–10.
17. Pei S., Minhajuddin M., Callahan K.P., Balys M., Ashton J.M., Neering S.J., Lagadinou E.D., Corbett C., Ye H., Liesveld J.L., O’Dwyer K.M., Li Z., Shi L., Greninger P., Settleman J., Benes C., Hagen F.K., Munger J., Crooks P.A., Becker M.W., Jordan C.T.: Targeting aberrant glutathione metabolism to eradicate human acute myelogenous leukemia cells. J Biol Chem. 2013; 288: 33542–33558.
18. Klein K., Kaspers G., Harrison C.J., Beverloo H.B., Reedijk A., Bongers M., Cloos J., Pession A., Reinhardt D., Zimmerman M., Creutzig U., Dworzak M., Alonzo T., Johnston D., Hirsch B., Zapotocky M., De Moerloose B., Fynn A., Lee V., Taga T., Tawa A., Auvrignon A., Zeller B., Forestier E., Salgado C., Balwierz W., Popa A., Rubnitz J., Raimondi S., Gibson B.: Clinical impact of additional cytogenetic aberrations, ckit and ras mutations, and treatment elements in pediatric t(8;21)-aml: results from an international retrospective study by the international Berlin–Frankfurt–Münster study group. J Clin Oncol. 2015; 20: 4247–4258.
19. Burnett A.K.: New induction and postinduction strategies in acute myeloid leukemia. Curr Opin Hematol. 2012; 19: 76–81.
20. Kagan V.E., Yalowich J.C., Borisenko G.G., Tyurina Y.Y., Tyurin V.A., Thampatty P., Fabisiak J.P.: Mechanism-based chemopreventive strategies against etoposide-induced acute myeloid leukemia: free radical/antioxidant approach. Mol Pharmacol. 1999; 56: 494–506.
21. Patel N.M., Nozaki S., Shortle N.H., Bhat-Nakshatri P., Newton T.R., Rice S., Gelfanov V., Boswell S.H., Goulet R.J., Sledge G.W., Nakshatri H.: Paclitaxel sensitivity of breast cancer cells with constitutively active NF-kappaB is enhanced by Ikappa-B alpha super-repressor and parthenolide. Oncogene. 2000; 19: 4159–4169.
22. deGraffenried L.A., Chandrasekar B., Friedrichs W.E., Donzis E., Silva J., Hidalgo M., Freeman J.W., Weiss G.R.: NF-kappa B inhibition markedly enhances sensitivity of resistant breast cancer tumor cells to tamoxifen. Ann Oncol. 2004; 15: 885–890.
23. Tietze F.: Enzymatic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues. Ann Biochem. 1969; 27: 502–522.
24. Papież M.A., Krzyściak W., Szade K., Bukowska-Straková K., Kozakowska M., Hajduk K., Bystrowska B., Dulak J., Jozkowicz A.: Curcumin enhances the cytogenotoxic effect of etoposide in leukemia cells through induction of reactive oxygen species. Drug Des Devel Ther. 2016; 10: 557–570.
25. Wurthwein G., Krumpelmann S., Tillmann B., Real E., Schulze-Westhoff P., Jurgens H., Boos J.: Population pharmacokinetic approach to compare oral and i.v. administration of etoposide. Anticancer Drugs. 1999; 10: 807–814.
26. Kim Y.R., Eom J.I., Kim S.J., Jeung H.K., Cheong J.W., Kim J.S., Min Y.H.: Myeloperoxidase expression as a potential determinant of parthenolide-induced apoptosis in leukemia bulk and leukemia stem cells. JPET. 2010; 335: 389–400.
27. Vlasova I.I., Feng W., Goff J.P., Giorgianni A., Do D., Gollin S.M., Lewis D.W., Kagan V.E., Yalowich J.C.: Myeloperoxidase-dependent oxidation of etoposide in human myeloid progenitor CD34+ cells. Mol Pharmacol. 2011; 79: 448–479.
28. Seo K.H., Ko H.M., Han A., Kim H.A., Choi J.H., Park S.J., Kim K.J., Lee H.K., Im S.Y.: Platelet-activating factor induces up-regulation of antiapoptotic factors in a melanoma cell line through nuclear factor-kb activation. Cancer Res. 2006; 66: 4681–4686.
29. Teufelhofer O., Weiss R.M., Parzefall W., Schulte-Hermann R., Micksche M., Berger W., Elbling L.: Promyelocytic HL60 cells express NADPH oxidase and are exellent targets in a rapid spectrophotometric microplate assay for extracellular superoxide. Toxicol Sci. 2003; 76: 376–383.
30. Skalska J., Brookes P.S., Nadtochiy S.M., Hilchey S.P., Jordan C.T., Guzman M.L., Maggirwar S.B., Briehl M.M., Bernstein S.H.: Modulation of cell surface protein free thiols: a potential novel mechanism of action of the sesquiterpene lactone parthenolide. PLoS One. 2009; 2: e8115.
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Authors and Affiliations

Monika A. Papież
1
Oliwia Siodłak
1
Wirginia Krzyściak
2

  1. Department of Cytobiology, Faculty of Pharmacy, Jagiellonian University Medical College, Kraków, Poland
  2. Department of Medical Diagnostic, Faculty of Pharmacy, Jagiellonian University Medical College, Kraków, Poland

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