An Internet application was developed to support the design process of the WLAN access network. The whole program was designed as a desktop application using the following languages: HTML, CSS and JavaScript. The main task of the application facilitates the planning process of the WLAN access network, including the location of multiple base stations. There is an example of using the program in the WLAN network planning process. The main purpose of this application as the ability to carry out network planning using multiple base stations and provide radio coverage for the entire area served by Wireless Internet Service Provider, is obtained.

The article presents a scientific research concerning the role and development of one of the most important leisure and recreation areas in Warsaw — Pole Mokotowskie, located near the city center, in three districts: Ochota, Śródmieście and Mokotów (part of the area is the Park of Józef Piłsudski). The research concentrates on determining the influence of contemporary spatial transformations of the park area on the spatial composition of the park.

The subject of the article is the design and practical implementation of the wireless mesh network. IQRF radio modules were used for the network design. The IQRF® technique has enabled the construction of a mesh network with the possibility of reconfiguration. The theoretical part contains a description of the IQRF® hardware solutions used. The practical scope includes the design part, where the configuration of the radio modules was carried out and the parameters of the radio network were set to allow its implementation in various topologies. Then, a wireless network consisting of 10 IQRF modules was launched in the P3 building of the Opole University of Technology. At this stage, configured radio modules were placed in selected rooms on all five floors of the building in order to carry out tests of the radio network constructed in this way. The tests included measuring the packet transmission delay time as well as the received signal strength. Research was carried out for several network topologies.

Telomeres are repetitive sequence structures at the ends of chromosomes. They consist of the double stranded DNA repeats followed by the short single stranded DNA. In humans and other verterbrates the telomeric sequence is composed of tandem of TTAGGG repeats. With each cells division telomeres shorten by up to 200 base pairs. Telomerase is an enzyme responsible for continuous cell growth and is repressed in most somatic cells, except proliferating progenitor cells, but in more than 85% of cancer cells telomerase expression is observed. Tumour cells with metastatic potential may demonstrate a high telomerase activity, allowing cells to escape from the inhibition of cell proliferation due to shortened telomeres. Determination of telomerase expres- sion was performed with the use of PCR ELISA in samples isolated from bovine leukaemia virus (BLV) infected cows. Telomerase activity was found in almost all investigated samples. The relative telomerase activity (RTA) was higher in infected cows than in healthy animals and the differences were statistically significant (α=0.05). In blood lymphocytes of BLV-infected cows the mean values of telomerase expression determined in real-time PCR were 3534.12 copies, in the healthy group there were 1010.10 copies and these differences were also statistically significant. For telomere length evaluation the Telomere PNA/FITC FISH and Telomere PNA/FITC FISH for flow cytometry were used. The mean fluorescence intensity of telomere sequences calculated on the surface of interphase nuclei of leukaemic blood lymphocytes was lower than that in the control animals and the difference was statistically significant. The mean length of telomeres in BLV- infected and healthy cows was 31.63 ± 12.62 and 38.4 ± 4.03, (p=0.112), respectively.

Dendritic cells (DCs) due to their ability to present antigens are essential during the immune response to infections. The aim of the study was to evaluate the influence of bovine leukaemia virus (BLV) infection on DC properties. Cytokine profiles of myeloid, plasmacytoid and mono- cyte derived DCs from BLV infected cattle were analysed. Concentrations of IL-6, IL-10, IL-12, IFN-γ, and TNF-α in DC cultures were measured by flow cytometry. Obtained results indicated activation of pDCs population, where a significant increase in production of the IFN-γ was shown. Meanwhile, a decrease in production of IFN-γ and increase in production of IL-10 were shown in mDCs; the main population responsible for antigens presentation. This may indicate a contribu- tory role of the population during the process of persistent infection. In MoDCs population a significant elevation in secretion of proinflammatory cytokines – IL-6 and TNF-α was noted.

Local service centre, defined as multi-functional public space providing access to everyday services, promoting social integration and building local identity, is an essential hub in the urban service sector. From the public policy perspective appropriate location and furnishing of local service centres is a basic condition for shaping territorial cohesion. This paper synthesises the to-date knowledge on mechanisms determining the development of service sector in cities, including new phenomena such as decentralisation of urban functions, urban shrinkage, remote services provision or commercialisation of lifestyle. Critical literature review enables the confrontation of various research perspectives and leads to distinguishing key factors affecting the development of urban local service centres in the first half of 21st century and defining the current challenges for public policy.