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Abstract

The aim of the study was to develop a method of laparoscopic embryo transfer in pigs and to compare different variants of this method. Two catheter diameters (1.6 mm and 1.0 mm), the method and site of embryo deposition (oviduct or uterus), the embryo development stage (2 – 4 cell or blastocyst), the method for oviduct or uterus stabilization, the potential for cryopreserved embryo transfer, the developmental potential of the embryos after transfer to the oviduct, patomorphology of the oviduct after transfer and possible clinical complications were taken into consideration. Two studies compared two variants of transfer to the uterus, and five variants of transfer to the fallopian tube. The transfer of embryos by the infundibulum may be of limited use due to handling problems and very low efficiency (pregnancy was not achieved). Very low efficiency was shown after transfer of vitrified embryos. Transfer to the fallopian tube by puncture of the fallopian tube, regardless of the developmental stage of the embryo, is the recommended method of embryo transfer. The histopathological examination of the fallopian tube revealed possible changes within the puncture site. The numerous clinical complications observed did not affect the effectiveness of the method.
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Authors and Affiliations

J. Wieczorek
1
E. Stodolak-Zych
2
ORCID: ORCID
K. Okoń
3
J. Koseniuk
4
M. Bryła
5
J. Jura
5
K. Poniedziałek-Kempny
5
I. Rajska
5
K. Sobol
5
M. Kotula Balak
1
M. Chmurska-Gasowska
1

  1. University Centre of Veterinary Medicine, University of Agriculture in Krakow, Mickiewicza 24/28, 30-059 Krakow, Poland
  2. Department of Biomaterials, Faculty of Materials Science and Ceramics, University of Science and Technology, Mickiewicza 30, 30-059 Krakow, Poland
  3. Chair of Pathomorphology, Collegium Medicum, Jagiellonian University, Grzegorzecka 16, 30-526 Krakow, Poland
  4. Artvimed Centre for Reproductive Medicine, Czyzowka 14, 30-526 Krakow, Poland
  5. Department of Reproductive Biotechnology and Cryoconservation, National Research Institute of Animal Production, Krakowska 1, 30-322 Balice/Krakow, Poland

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