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Abstract

Endopolyploidy is a condition of a cell containing reduplicated genetic material in its nucleus. Cells with the nuclei of different ploidy levels are often present within a single polysomatic organism. Endoreduplication is thus a modified cell cycle that omits cytokinesis and leads to chromatin replication in the endopolyploid cells. This study aimed to research the effect of salinity on endopolyploidy of Trifolium pratense and T. repens. Both species are important pasture legumes and belong to the genus Fabaceae with the well documented endopolyploidy occurence. Endopolyploidy levels in the seedlings treated with 0, 30, 60, 90 and 120 mM NaCl were investigated by flow cytometry. The seedling organs were evaluated during three ontogeny stages. The cytometric data plotted on a histogram showed the presence of 2C-16C nuclei in T. pratense and 2C-8C in T. repens. The hypothesis that salinity induces additional endocycles was not confirmed. Our results show that the distribution of nuclei among ploidy levels does not differ markedly between the treatment groups and the control ones. Additionally, only minor changes were observed among the endoreduplication indexes (EI) of plant organs after exposure to various salt concentrations. Endopolyploidy patterns within the salt-treated seedlings during ontogeny are similar to the controls. We suggest that endopolyploidy in Trifolium species is a conserved genetic trait, rather than an adaptation to salinity stress. The analyses of the roots of T'. pratense at stage III show that with the increased concentrations of NaCl the length of roots decreased, but no evident changes in endopolyploidy occured.

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Authors and Affiliations

V Kocova
D Bubanova
A Rakai
V Kolarcik
P Martonfi
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Abstract

The content of this paper is dedicated to the analysis of the flat planarity of forklift stacker’s track and cross sections of lanes between racks in a warehouse. These results will serve as a basis for a possible reconstruction of the track and racks and shall contribute to the overall reduction of costs related to an unexpected bad technical condition. The contribution aims to assess the geometric parameters of warehouse racks at the selected company operation in terms of their suitability for further use. The choice of the selected topic represents a relevant issue, which can be possibly encountered in daily practice related to the storage and transport processes of products. The measurements and processing of longitudinal profiles and cross-sections were made in the local coordinate and local vertical system. Points on the lower, middle and upper level of racks were measured for good and correct interpretation of results. Testing the measured positional change of poles is on the end of this paper. The immediate readiness of interest groups of subjects for adopting necessary actions to ensure the stability and safe operation of the whole network of lanes of the warehouse spaces is the expected contribution of the presented results.

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Authors and Affiliations

Slavomír Labant
Marcela Bindzárová Gergel’ová
Štefan Rákay
Erik Weiss
ORCID: ORCID
Jozef Zuzik

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