In order to identify the modal parameters of civil structures it is vital to distinguish the defective data from that of appropriate and accurate data. The defects in data may be due to various reasons like defects in the data collection, malfunctioning of sensors, etc. For this purpose Exploratory Data Analysis (EDA) was engaged toenvisage the distribution of sensor’s data and to detect the malfunctioning with in the sensors. Then outlier analysis was performed to remove those data points which may disrupt the accurate data analysis. Then Data Driven Stochastic Sub-space Identification (DATA-SSI) was engaged to perform the modal parameter identification. In the end to validate the accuracy of the proposed method stabilization diagrams were plotted. Sutong Bridge, one of the largest span cable stayed bridge was used as a case study and the suggested technique was employed. The results obtained after employing the above mentioned techniques are very valuable, accurate and effective.

An eukaryotic expression system of Congjiang pigs IFN-λ1 was constructed to obtain its expression in CHO-K1 cells and the inhibition effect of Congjiang pig IFN-λ1 on PRRSV proliferation was verified. The eukaryotic expression plasmid pEGFP-PoIFN-λ1 was constructed from the pig IFN-λ1 gene fragment and transfected into CHO-K1 cells. Expression was detected by fluorescence microscopy and Western blotting. The influence on the proliferation of PRRSV was assessed. The results of the study showed that the recombinant plasmid pEGFP-PoIFN-λ1 was constructed correctly. After transfection, green fluorescent signal was detected in CHO-K1 cells by fluorescence microscopy. Western blot analysis revealed that in cells at different time periods after transfection, porcine IFN-λ1 was expressed, with the highest expression observed 36 h after transfection. The antiviral activity of the supernatant after 36 h of transfection was determined by the micro cytopathic inhibition method, and the biological activity was 2.1×103 U/mL. Quantitative PCR was used to detect the proliferation of PRRSV, and the results showed that Congjiang pigs IFN-λ1 significantly inhibited the mRNA expression of PRRSV and viral proliferation in a dose- and time-dependent manner. This study established a Congjiang pig IFN-λ1 eukaryotic expression system, and the quantitative PCR method showed that it has a significant inhibitory effect on the proliferation of PRRSV, which lays a foundation for the future production of antiviral drugs and clinical application.