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Abstract

The proper functioning of the perinatal sucking reflex in calves is essential for the prevention of milk leakage into the rumen. The complex process behind its regulation is mediated at the gut level via multiple excitatory and inhibitory neurotransmitters, of which acetylcholine and nitric oxide are of fundamental importance. The aim of our study was to depict age-related alterations in the cholinergic and nitrergic innervation of the esophageal groove (EG) using immunohistochemistry and Real-Time PCR methods. We found out that the highest number of cholinergic nerve cells was present in the second trimester fetuses. From this developmental stage onward, their amount was gradually decreasing and reached the lowest value in 4-year-old cows. The same developmental pattern was observed for nitrergic nerve structures with the highest percentage of nitrergic neurons in the third trimester fetuses. Our observations prove that both neuronal populations are crucial for a proper closure of EG in calves. Therefore, their contribution to a general neuronal activity in the ENS diminishes with age as the high motility of a gastric groove is not necessarily required in older cattle.
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Bibliography

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Sanders KM, Ward SM (2019) Nitric oxide and its role as a non-adrenergic, non-cholinergic inhibitory neurotransmitter in the gastrointestinal tract. Br J Pharmacol 176: 212-227.
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Woliński J, Słupecka M, Weström B, Prykhodko O, Ochniewicz P, Arciszewski M, Ekblad E, Szwiec K, Ushakova, Skibo G, Kovalenko T, Osadchenko I, Goncharova K, Botermans J, Pierzynowski S (2012) Effect of feeding colostrum versus exogenous immunoglobulin G on gastrointestinal structure and enteric nervous system in newborn pigs. J Anim Sci 90: 327-330.
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Authors and Affiliations

M. Marszałek
1
T. Serzysko
1
W. Sienkiewicz
1

  1. Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-719 Olsztyn, Poland
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Abstract

The trapezius muscle (TRAP) belongs to the scapulothoracic group of muscles, which play a crucial role in the integrity and strength of the upper limb, trunk, head, and neck movements and, thus, in maintaining balance. Combined retrograde tracing (using fluorescent tracer Fast Blue, FB) and double-labelling immunohistochemistry were applied to investigate the chemical coding of motoneurons projecting to the porcine TRAP. FB-positive (FB+) motoneurons supplying the cervical (c-TRAP) and thoracic part (th-TRAP) of the right (injected with the tracer) TRAP were located within the IX-th Rexed lamina in the ipsilateral ventral horn of the grey matter of the spinal medulla. Immunohistochemistry revealed that nearly all the neurons were cholinergic in nature [choline acetyltransferase (CHAT)- or vesicular acetylcholine transporter (VACHT)-positive]. Many retrogradelly labelled neurons displayed also immunoreactivity to calcitonin gene-related peptide (CGRP; approximately 68% of FB+ neurons). The smaller number of nerve cells (5%, 3%, 2% or 1%, respectively) stained for nitric oxide synthase (n-NOS), vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY) and substance P (SP). The retrogradely labelled neurons were closely apposed by nerve fibres expressing immunoreactivity to CHAT, VACHT, CGRP, SP, DβH, VIP, n-NOS, NPY, GAL, Leu-Enk and Met-Enk. Taking into account the clinical relevance of TRAP, the present results may be useful in designing further research aimed at the management of various dysfunctions of the muscle.
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Authors and Affiliations

A. Dudek
1
W. Sienkiewicz
1
E. Lepiarczyk
2
J. Kaleczyc
1

  1. Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-719 Olsztyn, Poland
  2. Department of Human Physiology and Pathophysiology, School of Medicine, Collegium Medicum,University of Warmia and Mazury in Olsztyn, Warszawska 30, 10-082, Olsztyn, Poland
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Abstract

The present study investigated the distribution and chemical properties of nerve cell bodies within the trunk of the vagus nerve in juvenile female pigs (n=4) using double-labelling immunofluorescence. The neurons appeared mostly as single cells or formed streaks of cells or small ganglia. Many of the perikarya were cholinergic (VAChT-positive; VAChT+) or adrenergic (DβH+) in nature and no SP+ or CGRP+ neurons were encountered. There were no distinct left-right differences regarding the number and chemical coding of the neuronal somata, however, these characteristics significantly varied between particular nerve segments investigated. The vagosympathetic trunks, and thoracic and abdominal segments of the vagus nerve contained on average (the numerical values represent the means for both the left and right corresponding nerve segments) 142, 236, and 111 PGP 9.5-positive neurons, respectively. Proportions of cholinergic and adrenergic neurons were as follows: 0% and 100%, 54.2% and 33.2%, and 52.8% and 35.4%, respectively. Relatively many neurons in the thoracic and abdominal segments stained also for NOS (39.2% and 39.9%, respectively). It remains to be determined whether the porcine intravagal neurons represent a developmental relic, or whether they have any specific functional significance.
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Authors and Affiliations

W. Sienkiewicz
1
M. Klimczuk
1
M. Majewska
2
J. Kaleczyc
1

  1. Department of Animal Anatomy, Faculty of Veterinary Medicine,University of Warmia and Mazury, Oczapowskiego 13, 10-719 Olsztyn, Poland
  2. Department of Human Physiology and Pathophysiology, School of Medicine,University of Warmia and Mazury in Olsztyn, Warszawska 30, 10-082 Olsztyn, Poland
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Abstract

The present study investigated the expression of androgen receptor (AR) in neurons of the anterior pelvic ganglion (APG) and celiac-superior mesenteric ganglion (CSMG; ganglion not involved in the innervation of reproductive organs) in the male pig with quantitative real-time PCR (qPCR) and immunohistochemistry. qPCR investigations revealed that the level of AR gene expression in the APG tissue was approximately 2.5 times higher in the adult (180-day-old) than in the juvenile (7-day-old) boars. Furthermore, in both the adult and juvenile animals it was sig- nificantly higher in the APG than in CSMG tissue (42 and 85 times higher, respectively). Immu- nofluorescence results fully confirmed those obtained with qPCR. In the adult boars, nearly all adrenergic (DβH-positive) and the majority of non-adrenergic neurons in APG stained for AR. In the juvenile animals, about half of the adrenergic and non-adrenergic neurons were AR-posi- tive. In both the adult and juvenile animals, only solitary CSMG neurons stained for AR. The present results suggest that in the male pig, pelvic neurons should be considered as an element of highly testosterone-dependent autonomic circuits involved in the regulation of urogenital func- tion, and that their sensitization to androgens is a dynamic process, increasing during the prepu- bertal period.

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Authors and Affiliations

J. Kaleczyc
N. Kasica-Jarosz
Z. Pidsudko
A. Przyborowska
W. Sienkiewicz
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Abstract

The development of the enteric nervous system (ENS) is still a valid and intensely studied issue. However, literature in the field has no data on this topic in the dog. The present investiga- tions were performed in three groups of fetuses from mongrel dogs – from the third, sixth- -seventh, and ninth week of pregnancy – and in 3-5-day-old puppies (3 specimens for each age group). The tissues (the medial parts of the duodenum, jejunum, and ileum with the cecum and a small portion of the adjacent ascending colon) were cut using a cryostat and the sections were processed for single- and double-labeling immunohistochemistry using antisera against acetylat- ed tubulin (AcTub), vesicular acetylcholine transporter (VAChT), nitric oxide synthase (NOS), vasoactive intestinal polypeptide (VIP), galanin (GAL), neuropeptide Y (NPY), substance P (SP), and calcitonin gene-related peptide (CGRP). In the 3-week-old fetuses, some oval cells invading the gut wall were found. From the seventh week of pregnancy onwards, two different enteric ganglia were present: submucosal and myenteric. The estimated number of nerve elements in the 9-week-old fetuses was much higher than that observed in the 6-7-week-old individuals. There was no significant difference in the estimated number of nerve structures between the 9-week-old fetuses and the 3-5-day-old puppies. The colonization pattern and the develop- ment of the ENS in the canine small intestine are very similar to those observed in other mam- mals. However, a few exceptions have been confirmed, regarding the time of appearance of the VIP-, GAL-, and CGRP-immunoreactive neurons, and their distribution in different portions of the canine bowel during development.
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Authors and Affiliations

T. Serzysko
1
A. Skwarek
1
E. Chudziak
1
M. Malina
1
J. Kaleczyc
1
W. Sienkiewicz
1

  1. Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland
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Abstract

European beaver (Castor fiber), the largest rodent species inhabiting a wide area of Eurasia, feeds mainly on dry parts of plants, bark or wood. Such kind of nourishment needs to be properly digested in each part of the gastrointestinal tract. The time of stomach digestion, which directly influences all the following steps of the digestion process, is precisely controlled by the pylorus and its innervation. However, virtually no data is available on the organization of the enteric nervous system in most of the wild animal species, including beavers. On the other hand, a pecu- liar diet consumed by beavers, suggests that the arrangement of their stomach intramural nerve elements can be atypical. Therefore, the present study investigated the distribution and chemical coding of neurons and nerve fibers in the pylorus of the European beaver.

The experiment was performed on stomachs obtained from a group of 6 beavers caught in Northeastern region of Poland (due to beaver overpopulation). Pyloric wall tissue cryosections were double immunostained with a mixture of antibodies against pan-neuronal marker PGP 9.5 (to visualize enteric neurons) and ChAT (cholinergic marker), nNOS (nitrergic marker), SP, CGRP, Gal (peptidergic markers).

Confocal microscopy analysis revealed that the majority of enteric nerve cells were clustered forming submucosal and myenteric ganglia and all the studied substances were expressed (in various amounts) in these neurons.

We conclude, that the anatomical arrangement and chemical coding of intramural nerve elements in the beaver pylorus resemble those found in other mammalian species.

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Authors and Affiliations

M. Zalecki
K. Makowska
Z. Gizejewski
M. Klimczuk
A. Franke-Radowiecka
N. Kasica-Jarosz
W. Sienkiewicz
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Abstract

The present study investigated the chemical coding of neurons and nerve fibres in local laryngeal ganglia in pigs (n=5) using double-labelling immunohistochemistry. Virtually all the neurons were cholinergic in nature (ChAT- or VAChT-positive). Only very solitary, small nerve cells (presumably representing interneurons) stained intensely for adrenergic marker, DβH. Many neurons also contained immunoreactivity for NOS (91%), VIP (62.7%), NPY (24.7%), galanin (10%), SP (1.3%) and CGRP (5.3%). No neurons expressing somatostatin or Leu-enkephalin were observed. Nearly all the neuronal somata were densely supplied with varicose cholinergic nerve terminals, which presumably represented preganglionic axons, and some of them were also closely apposed with CGRP- and/or SP-positive varicose nerve endings, which were putative collaterals of extrinsic primary sensory fibres. In conclusion, this study has revealed that intrinsic neurons in the porcine larynx, like in many other mammalian species studied, should be classified as parasympathetic cholinergic neurons expressing biologically active substances, predominantly NOS and VIP. Furthermore, they are likely to receive inputs from not only preganglionic neurons but also primary sensory nerve cells. Finally, it appears that the information on the occurrence of the local laryngeal ganglia should be regularly included in textbooks dealing with the cranial portion of the parasympathetic nervous system in mammals.
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Authors and Affiliations

W. Sienkiewicz
1
M. Klimczuk
1
M. Gulbinowicz-Gowkielewicz
2
E. Lepiarczyk
3
J. Kaleczyc
1

  1. Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-719 Olsztyn, Poland
  2. Department of Otolaryngology and Laryngological Oncology, Voivodal Specialistic Hospital, Żołnierska 18, 10-561 Olsztyn, Poland
  3. Department of Human Physiology and Pathophysiology, School of Medicine, University of Warmia and Mazury in Olsztyn, Warszawska 30, 10-082, Olsztyn, Poland
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Abstract

This article examines the analogies, and more specifically the historical 'theatre of the imagination', between Tytus Czyżewski's Robespierre/Rhapsody (1927) and Stanisław Wyspiański's poetic dramas Rhapsodies (Kazimierz the Great and Bolesław the Bold). Each of those poems foregrounds its principal historical character. Wyspiański's dramatic poems, commonly known as Rhapsodies, focus on Kazimierz the Great, Bolesław the Bold, and Piast. kings of pivotal significance in his vision of Poland's historical destiny. Twenty years later Tytus Czyżewski, an acclaimed avant-garde painter and poet, composed a poetic-essayistic salmagundi, in which he sought to render in a similarly elevated style and condensed dialogue the drama of the leaders of the French Revolution, Robespierre and Danton. While Robespierre has to face, apart from some common people, God, the Spirit and Judges that sit in judgment on him, the final section of Rhapsody evokes Juliusz Słowacki. A monologue, mimicking his lofty verse, establishes a metaphorical common thread in Polish history – from the days of mail-clad knights to the wretched everyday life in the trenches – set against a broad background of wars, destruction and the French Revolution. For Czyżewski the French Revolution was a ground-breaking event, the first act of a great historical process that ushered in the Modern Age with its ideas of progress, reason, freedom, social justice, the elimination of poverty. It continues to inspire mankind with the hope that even a most ambitious change is possible. For Wyspiański, on the other hand, the grand project of human emancipation does give rise to doubts whether a wholesale obliteration of the Old is justified and to questions about God, free will, theodicy and destiny, and the 'tyranny of reason'. The differences between the two philosophies of history – Wyspiański's, from the turn of the 19th century, and Czyżewski's, representative of the artistic and intellectual climate of the late 1920s – are no doubt profound, and yet, what both of them seem to share is a deep concern with the relevance of history for the present and for designing the future.

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Authors and Affiliations

Barbara Sienkiewicz
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Abstract

The presented paper concerns a novel concept of hybrid piezoelectric motor based on electroactive lubrication principle. Its structure is combined of quasi-static and resonance piezoelectric actuators, synchronizing their work to generate the rotary movement. The hybrid motor topology is compared to the existing piezoelectric motors, regarding its field of applications in embedded systems with very high security requirements. The electroactive lubrication principle is briefly presented with regards to optimization of the hybrid motor. The performance principle of the hybrid motor is described in terms of its working cycle. The assembling process of the prototype hybrid motor is briefly explained with emphasis put on the frequency and impedance tuning of the applied quasi-static and resonance piezoelectric actuators. Next, the hybrid motor power supply system is described and chosen measured performance characteristics are presented. Finally, conclusions concerning the features of the tested prototype hybrid motor and possible solutions of the faced issues, during assembling and testing, are presented.

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Authors and Affiliations

Jean-Francois Rouchon
Dominique Harribey
Duc-Hoan Tran
Roland Ryndzionek
Łukasz Sienkiewicz
Mieczysław Ronkowski
Michal Michna
Grzegorz Kostro
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Abstract

Autonomic nervous system of the pelvis is still poorly understood. Every year more and more pelvic procedures are carried out on patients suff ering from diff erent pelvic disorders what leads to numerous pelvic dysfunctions. Authors tried to review, starting from historical and clinical background, the most important reports on anatomy of the pelvic autonomic plexuses. We also pay attention to complete lack of knowledge of students of medicine on the autonomic nervous structures in the area studied. We present anatomical description of the pelvic plexuses including their visceral branches and anatomy of surrounding pelvic tissues which still remains unclear. More and more attention is paid to the topography of the plexuses specially because of new pain releasing techniques — neurolysies.

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Authors and Affiliations

Justyna Sienkiewicz-Zawilińska
Jarosław Zawiliński
Lourdes Niroya Kaythampillai
Marcin Jakiel
Jacenty Urbaniak
Tomasz Bereza
Wojciech Kowalski
Marios Loukas
Jerzy Walocha

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