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Number of results: 9
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Helmintofauna of turbot Scophthalmus maximus (Linnaeus, 1758) from the southern Baltic Sea including new data

M. Skrzypczak L. Rolbiecki
Polish Journal of Veterinary Sciences | 2015 | No 3 | DOI: 10.1515/pjvs-2015-0077
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Authors and Affiliations

M. Skrzypczak
L. Rolbiecki

Babesia gibsoni infection in dogs in Poland

O. Teodorowski M. Kalinowski M. Skrzypczak K. Witt J. Madany S. Winiarczyk Ł. Adaszek
Polish Journal of Veterinary Sciences | 2020 | vol. 23 | No 3 | 469-471 | DOI: 10.24425/pjvs.2020.134694
Keywords Babesia gibsoni dogs PCR vector-borne diseases
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Abstract

Canine babesiosis is a tickborne, protozoal, haemoparasitic disease. Babesia organisms are frequently classified as either large (B. canis) or small (B. gibsoni). The aim of this study was an attempt to detect B. gibsoni DNA in blood samples taken from dogs suspected of suffering from tick-borne diseases. 216 samples were tested using PCR, of which, in 99 of them B. canis DNA was detected, whereas in 3 of them B. gibsoni was detected. Positive PCR results for B. gibsoni were confirmed using a Qube MDx real-time analyzer. The results indicate that infections with this B. gibsoni should be taken into account and included in the differential diagnosis of vector-borne diseases in dogs in Poland, and that the accurate identification of the species of parasite causing the infection is crucial for developing the correct treatment regimen and prognosis.

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Authors and Affiliations

O. Teodorowski
M. Kalinowski
M. Skrzypczak
K. Witt
J. Madany
S. Winiarczyk
Ł. Adaszek

Lyme disease in Bernese Mountain Dogs. Is it a real problem?

Ł. Adaszek M. Pisarek M. Kalinowski M. Skrzypczak M. Winiarczyk B. Abramowicz S. Winiarczyk
Polish Journal of Veterinary Sciences | 2022 | vol. 25 | No 4 | 639-647 | DOI: 10.24425/pjvs.2022.142036
Keywords Lyme disease vector-borne diseases dogs
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Abstract

Borreliosis is the most frequently diagnosed tick-borne disease caused by spirochete bacteria belonging to the genus Borreliae - Borrelia burgdorferi sensu stricto (s.s.), Borrelia afzelii and Borrelia garinii. Clinical manifestations in dogs include fever, lameness, polyarthritis and glomerulonephritis. Diagnosis is mainly serological and is based on an immunoenzymatic test followed by a Western blot confirmatory test. Early treatment with antibiotics such as doxycycline or amoxicillin, for four weeks, usually reduces the risk of chronic disease. Tick control, including tick repellents, is highly reliable in preventing transmission. Vaccines are available to reduce transmission and the clinical manifestations of infection in dogs. Bernese Mountain Dogs are a breed that often test positive for antibodies against B. burgdorferi without showing any clinical symptoms of the disease. Quantitative determination of the immunoglobulin level for spirochetes has indicated that Bernese Mountain Dogs may have an increased susceptibility to Borrelia spp. infections of a hereditary nature.
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Authors and Affiliations

Ł. Adaszek
1
M. Pisarek
1
M. Kalinowski
1
M. Skrzypczak
2
M. Winiarczyk
3
B. Abramowicz
4
S. Winiarczyk
1
  1. Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-612 Lublin, Poland
  2. Second Department of Gynecology, Medical University of Lublin, 20-954 Lublin, Poland
  3. Department of Vitreoretinal Surgery, Medical University of Lublin, 20-097 Lublin, Poland
  4. Department and Clinic of Animal Internal Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-612 Lublin, Poland

Clinical course of granulocytic anaplasmosis in hunting dogs

O. Teodorowski S. Winiarczyk P. Debiak M. Skrzypczak Ł. Mazurek Ł. Adaszek
Polish Journal of Veterinary Sciences | 2021 | vol. 24 | No 2 | 175-181 | DOI: 10.24425/pjvs.2021.136807
Keywords Anaplasma phagocytophilum hunting dog vector-borne disease
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Abstract

The aim of this study was to analyze cases of granulocytic anaplosmosis diagnosed in 53 hunting dogs in Poland. Medical records of dogs naturally infected with Anaplasma phagocytophilum were retrospectively evaluated with regard to clinical signs and laboratory abnormalities at the time of presentation, therapy and course of disease. The most common clinical signs in A. phagocytophilum-positive dogs included in the study were lethargy (100%), inappetence (94%) and fever (92.5%). Thrombocytopenia was the most common laboratory abnormality (100%), followed by a drop in haematocrit level (79.3%) and increased AST activity (75.5%).
Of the 53 infected dogs, 51 (96%) recovered and two dogs (with neurological symptoms) died.
Analysis of these cases indicates that A. phagocytophilum infection must be considered in differential diagnosis in dogs living in Poland, especially in hunting dogs with thrombocyto- penia and Ixodes ricinus tick invasions.
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Authors and Affiliations

O. Teodorowski
1
S. Winiarczyk
2
P. Debiak
3
M. Skrzypczak
4
Ł. Mazurek
2
Ł. Adaszek
2
  1. “Teodorowscy” Veterinary Clinic in Mikołów, Poland
  2. Department of Epizootiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Głęboka 30, 20-612 Lublin, Poland
  3. Department and Clinic of Animal Surgery, Laboratory of Radiology and Ultrasonography, University of Life Sciences, Głęboka 30, 20-612 Lublin, Poland
  4. Second Department of Gynecology, Prof. F. Skubiszewski University School of Medicine, Lublin, Poland

Comparison of serum protein profiles of Borrelia burgdorferi-positive Bernese Mountain Dogs and dogs of other breeds using MALDI-TOF technique

M. Pisarek M. Kalinowski M. Skrzypczak Ł. Mazurek K. Michalak D. Pietras-Ożga B. Dokuzeylü S. Winiarczyk Ł. Adaszek
Polish Journal of Veterinary Sciences | 2023 | vol. 26 | No 3 | 493–496 | DOI: 10.24425/pjvs.2023.145041
Keywords Borrelia burgdorferi Bernese Mountain dogs mass spectrometry
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Abstract

The aim of the study was to compare the serum protein profile of Bernese Mountain Dogs (BMDs) reacting positive for Bb in snap testing with the serum protein profile of dogs of other breeds (healthy and with clinical borreliosis) using the MALDI time-of-flight (MALDI-TOF) technique. The observations included five groups of dogs. BMDs reacting positively to Bb in snap serological testing and showing symptoms of borreliosis (group 1), BMDs for which no borreliosis symptoms were determined but with seropositivity for Bb determined with snap serological tests (group 2), clinically healthy BMDs with no antibodies for Bb found in the serum (group 3), five dogs of different breeds, reacting positively in serological testing, in which borreliosis symptoms were observed (group 4), clinically healthy dogs of different breeds with negative reaction in tests towards Bb (group 5). A proteomic analysis demonstrated the presence of five identical protein fractions among all five groups. An additional two protein fractions of approximately 7.630 and 15.260 kDa were found in all the serum samples obtained from the dogs positive for borrelia in a snap test, both in those exhibiting symptoms of borreliosis, and seropositive BMDs not presenting symptoms of the disease. These two additional protein fractions may be used to differentiate between seropositive and seronegative B. burgdorferi dogs and may be considered a seropositivity marker, however, it cannot be used to differentiate between animals with the clinical form of the disease and those that are only seropositive.
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Bibliography

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Authors and Affiliations

M. Pisarek
1
M. Kalinowski
1
M. Skrzypczak
2
Ł. Mazurek
1
K. Michalak
1
D. Pietras-Ożga
1
B. Dokuzeylü
3
S. Winiarczyk
1
Ł. Adaszek
1
  1. Department of Epizoology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine of the University of Life Sciences in Lublin, ul. Głęboka 30, 20-612 Lublin, Poland
  2. Second Department of Gynecology, Medical University of Lublin, 20-954 Lublin, Poland
  3. Department of Internal Medicine, Veterinary Faculty, Istanbul University-Cerrahpasa, 34320 Avcilar Campus, Avcilar, Istanbul, Turkey

Molecular analysis of a fragment of gene E1B 19K of canine adenovirus 2 (CAV-2) isolated from dogs with symptoms of cough

M. Kalinowski Ł. Adaszek S. Winiarczyk P. Miłoszowska M. Skrzypczak A. Ziętek-Barszcz J. Kutrzuba Z. Grądzki
Polish Journal of Veterinary Sciences | 2012 | No 3 | DOI: 10.2478/v10181-012-0066-7
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Authors and Affiliations

M. Kalinowski
Ł. Adaszek
S. Winiarczyk
P. Miłoszowska
M. Skrzypczak
A. Ziętek-Barszcz
J. Kutrzuba
Z. Grądzki

Detection of Borrelia burgdorferi sensu lato DNA in the blood of wild bison from Białowieza Primeval Forest in eastern Poland

M. Staniec Ł. Adaszek S. Winiarczyk B. Dzięgiel M. Krzysiak M. Skrzypczak M. Adaszek D. Wyłupek
Polish Journal of Veterinary Sciences | 2014 | No 4 | DOI: 10.2478/pjvs-2014-0103
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Authors and Affiliations

M. Staniec
Ł. Adaszek
S. Winiarczyk
B. Dzięgiel
M. Krzysiak
M. Skrzypczak
M. Adaszek
D. Wyłupek

The identification of Anaplasma spp. isolated from fallow deer (Dama dama) on a free-range farm in eastern Poland

Ł. Adaszek S. Winiarczyk J. Ziętek P. Klimiuk M. Skrzypczak M. Górna
Polish Journal of Veterinary Sciences | 2012 | No 2 | DOI: 10.2478/v10181-012-0060-0
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Authors and Affiliations

Ł. Adaszek
S. Winiarczyk
J. Ziętek
P. Klimiuk
M. Skrzypczak
M. Górna

Application of Matrix-assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry for Identification of Coagulase-negative Staphylococci Isolated from Milk of Cows with Subclinical Mastitis

T. Banach Ł. Adaszek S. Winiarczyk J. Ziętek M. Bochniarz P. Łyp W. Wawron B. Furmaga M. Skrzypczak
Polish Journal of Veterinary Sciences | 2016 | No 3 | DOI: 10.1515/pjvs-2016-0078
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Authors and Affiliations

T. Banach
Ł. Adaszek
S. Winiarczyk
J. Ziętek
M. Bochniarz
P. Łyp
W. Wawron
B. Furmaga
M. Skrzypczak

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