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Abstract

Neospora caninum ( N. caninum) is the etiologic agent of neosporosis, a potential cause of severe reproductive disorders in cattle, small ruminants, equines, wild animals and canids across the world. The current study is performed to estimate molecular prevalence of N. caninum in small ruminants and equines that had abortion in Kurdistan region of Iraq. A total of 64 tissue samples (brain, placenta, heart, lung and liver) were taken from aborted foetuses, with a total of 122 dam blood samples taken from 63 sheep, 39 goats, 12 mares and 8 jennies in local breed fields. Besides, a risk factor analysis for N. caninum positive animals was performed. The observed prevalence of N. caninum DNA in the blood of sheep, goats, horses and donkeys were 20.6%, 17.9%, 21.4% and 25.0%, respectively, and 19.3%, 17.6%, 18.1 and 20.0% in the aborted foetuses of the animals, respectively. Moreover, occurrence of N. caninum was 20.3% in the blood of aborted dams, while it was 18.7% in their aborted foetuses. Confirmatory analysis was also done through constructing a phylogenetic tree to compare the partial sequences of the Nc-5 gene in our isolates (OP771519, OP771520, OP771521 and OP771522) with the GenBank sequences. This showed 98-100% sequence identity with other N. caninum strains in the GenBank database. Older small ruminants and equines had a higher risk of being positive for N. caninum and exposure to dogs were considered as significant risk factors for N. caninum infection in the studied animals (p<0.05). Thus, the results of this study suggest that N. caninum is one of the microbial abortive agents in small ruminants and equines in Kurdistan region of Iraq. It is hoped that the results of this study will help to control animal abortion in livestock and reduce the economic losses.
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Bibliography

  1. Ahmed NE, Al-Akabway LM, Ramadan MY, El-Gawad SM, Moustafa MM (2017) Serological and PCR-sequencing assays for diagnosis of Toxoplasma gondii and Neospora caninum infecting camels in Egypt. Benha Vet Med J 33: 200-210.
  2. AL-Badrani BA, AL-Farwachi MI, AL-Hankawai OK (2012) Detection of Toxoplasma gondii and Neospora caninum antibodies in cattle in Mosul city, Iraq. Al-Qadisiyah J Vet Med Sci 11: 46-50.
  3. Al-Farwachi MI, Al-Badrani BA, Al-Khafaji WS (2018) Serodiagnosis of ovine neosporosis in Mosul city , Iraq. Eurasian J Vet Sci 28: 190-193.
  4. Al-Farwachi MI, Al-Hankawe OK, Al-Khafaji WS (2012) Serodiagnosis of Bovine Neosporosis in Mosul City, Iraq. Assiut Vet Med J 58: 1-4.
  5. Al-Shaeli SJ, Ethaeb AM, Gharban HA (2020) Molecular and histopathological identification of ovine neosporosis (Neospora caninum) in aborted ewes in Iraq. Vet World 13: 597-603.
  6. Almería S, Serrano-Perez B, Darwich L, Domingo M, Mur-Novales R, Regidor-Cerrillo J, Cabezón O, Pérez-Maillo M, Lopez-Helguera I, Fernández-Aguilar X, Puig-Ribas M, Ortega-Mora LM, García-Ispierto I, Dubey JP, López-Gatius F (2016) Foetal death in naive heifers inoculated with Neospora caninum isolate Nc-Spain7 at 110 days of pregnancy. Exp Parasitol 168: 62-69.
  7. Amdouni Y, Abedennebi I, Amairia S, Abdelkader A, Chandoul W, Gharbi M (2022) First molecular detection of Neospora caninum from naturally infected slaughtered camels in Tunisia. Vet Med Sci 8: 2241-2247.
  8. Amdouni Y, Rjeibi MR, Awadi S, Rekik M, Gharbi M (2018) First detection and molecular identification of Neospora caninum from naturally infected cattle and sheep in North Africa. Transbound Emerg Dis 65: 976-982.
  9. Anderson JA, Alves DA, Cerqueira-Cézar CK, da Silva AF, Murata FH, Norris JK, Howe DK, Dubey JP (2019) Histologically, immunohistochemically, ultrastructurally, and molecularly confirmed neosporosis abortion in an aborted equine fetus. Vet Parasitol 270: 20-24.
  10. Antonello AM, Pivoto FL, Camillo G, Braunig P, Sangioni LA, Pompermayer E, Vogel FS (2012) The importance of vertical transmission of Neospora sp. in naturally infected horses. Vet Parasitol 187: 367-370.
  11. Bartley PM, Guido S, Mason C, Stevenson H, Chianini F, Carty H, Innes EA, Katzer F (2019) Detection of Neospora caninum DNA in cases of bovine and ovine abortion in the South-West of Scotland. Parasitol 146: 979-982.
  12. Bártová E, Sedlák K, Kobédová K, Budíková M, Joel Atuman Y, Kamani J (2017) Seroprevalence and risk factors of Neospora spp. and Toxoplasma gondii infections among horses and donkeys in Nigeria, West Africa. Acta Parasitol 62: 606-609.
  13. Basso W, Holenweger F, Schares G, Müller N, Campero LM, Ardüser F, Moore-Jones G, Frey CF, Zanolari P (2022) Toxoplasma gondii and Neospora caninum infections in sheep and goats in Switzerland: Seroprevalence and occurrence in aborted foetuses. Food Waterborne Parasitol 28: e00176.
  14. Basso W, Venturini L, Venturini MC, Hill DE, Kwok OCH, Shen SK, Dubey JP (2001) First isolation of Neospora caninum from the feces of a naturally infected dog. J Parasitol 87: 612-618.
  15. Beck HP, Blake D, Dardé ML, Felger I, Pedraza-Díaz S, Regidor-Cerrillo J, Gómez-Bautista M, Ortega-Mora LM, Putignani L, Shiels B, Tait A, Weir W (2009) Molecular approaches to diversity of populations of apicomplexan parasites. Int J Parasitol 39: 175-189.
  16. Cong W, Nie LB, Qin SY, Wang WL, Qian AD, Meng QF (2018) Prevalence of Neospora spp. in donkeys in China. Parasite 25, 16.
  17. Costa RC, Mesquita LP, Nunes MV, Oliveira IM, Oliveira LF, Souza AR, Maiorka PC, Varaschin MS (2018) Neospora caninum bioassay in gerbils using placental tissues from naturally infected goats. Vet Parasitol 249: 70-73.
  18. Dahourou LD, Gbati OB, Savadogo M, Yougbare B, Dicko A, Combari AH, Kamga-Waladjo AR (2019) Prevalence of Toxoplasma gondii and Neospora caninum infections in households sheep “Elevage en case” in Dakar, Senegal. Vet World 12: 1028-1032.
  19. Diakou A, Papadopoulos E, Panousis N, Karatzias C, Giadinis N (2013) Toxoplasma gondii and Neospora caninum seroprevalence in dairy sheep and goats mixed stock farming. Vet Parasitol 198: 387-390.
  20. Della Rosa P, Fiorentino MA, Morrell EL, Scioli MV, Paolicchi FA, Moore DP, Cantón GJ, Hecker YP (2021) Neospora caninum and Toxoplasma gondii as causes of reproductive losses in commercial sheep flocks from Argentina. Curr Res Parasitol Vector Borne Dis 1: 100057.
  21. Duarte PC, Conrad PA, Barr BC, Wilson WD, Ferraro GL, Packham AE, Carpenter TE, Gardner IA (2004) Risk of transplacental transmission of Sarcocystis neurona and Neospora hughesi in California horses. J Parasitol 90: 1345-1351.
  22. Dubey JP (2003) Review of Neospora caninum and neosporosis in animals. Korean J Parasitol 41: 1-16.
  23. Dubey JP, Buxton D, Wouda W (2006) Pathogenesis of Bovine Neosporosis. J Comp Pathol 134: 267-289.
  24. Dubey JP, Hemphill A, Calero-Bernal R, Schares G (2017) Neosporosis in animals, 1st ed., CRC Press, Taylor and Francis group.
  25. Dubey JP, Jenkins MC, Ferreira LR, Choudhary S, Verma SK, Kwok OC, Fetterer R, Butler E, Carstensen M (2014) Isolation of viable Neospora caninum from brains of wild gray wolves (Canis lupus). Vet Parasitol 201: 150-153.
  26. Dubey JP, Schares G (2011) Neosporosis in animals-the last five years. Vet Parasitol 180: 90-108.
  27. Dubey JP, Schares G, Ortega-Mora LM (2007) Epidemiology and control of neosporosis and Neospora caninum. Clin Microbiol Rev 20: 323-367.
  28. Faraj AA, Ghattof HH (2018) Diagnosis of Neospora caninum using ELIZA and study of histopathological changes in dairy goat in Wasit province: Iraq. J Entom Zool Stud 6: 1256-1259.
  29. Gazzonis AL, Garcia GA, Zanzani SA, Ortega Mora LM, Invernizzi A, Manfredi MT (2016) Neospora caninum infection in sheep and goats from north-eastern Italy and associated risk factors. Small Rumin Res 140: 7-12.
  30. Gennari SM, Pena HF, Lindsay DS, Lopes MG, Soares HS, Cabral AD, Vitaliano SN, Amaku M (2016) Prevalence of antibodies against Neospora spp. and Sarcocystis neurona in donkeys from northeastern Brazil. Rev Bras Parasitol Vet 25: 109-111.
  31. Gharekhani J, Yakhchali M, Berahmat R (2020) Neospora caninum infection in Iran (2004-2020): A review. J Parasit Dis 44: 671-686.
  32. Gharekhani J, Yakhchali M, Heidari R (2022) Molecular detection and phylogenetic analysis of Neospora caninum in various hosts from Iran. Comp Immunol Microbiol Infect Dis 80: 101737.
  33. Ghattof HH, Faraj AA (2015) Seroprevalence of Neospora caninum in goats in Wasit province Iraq. Int J Curr Microbiol Appl Sci 4: 182-191.
  34. Gondim LF (2006) Neospora caninum in wildlife. Trends Parasitol 22: 247-252.
  35. Gondim LF, McAllister MM (2022) Experimental Neospora caninum Infection in Pregnant Cattle: Different Outcomes Between Inoculation With Tachyzoites and Oocysts. Front Vet Sci 9: 911015.
  36. González-Warleta M, Castro-Hermida JA, Regidor-Cerrillo J, Benavides J, Álvarez-García G, Fuertes M, Ortega-Mora LM, Mezo M (2014) Neospora caninum infection as a cause of reproductive failure in a sheep flock. Vet Res 45: 88.
  37. Irehan B, Sonmez A, Atalay MM, Ekinci AI, Celik F, Durmus N, Ciftci AT, Simsek S (2022) Investigation of Toxoplasma gondii, Neospora caninum and Tritrichomonas foetus in abortions of cattle, sheep and goats in Turkey: Analysis by real-time PCR, conventional PCR and histopathological methods. Comp Immunol Microbiol Infect Dis 89: 101867.
  38. Japa O, Morand S, Karnchanabanthoeng A, Chaisiri K, Ribas A (2018) Detection of Neospora caninum (Toxoplasmatidae) in wild small mammals from Thailand. Folia Parasitologica 65.
  39. King JS, Jenkins DJ, Ellis JT, Fleming P, Windsor PA, Šlapeta J (2011) Implications of wild dog ecology on the sylvatic and domestic life cycle of Neospora caninum in Australia. Vet J 188: 24-33.
  40. Langoni H, Greca HJ, Guimarães FF, Ullmann LS, Gaio FC, Uehara RS, Rosa EP, Amorim RM, Da Silva RC (2011) Serological profile of Toxoplasma gondii and Neospora caninum infection in commercial sheep from São Paulo State, Brazil. Vet Parasitol 177: 50-54.
  41. Locatelli-Dittrich R, Dittrich JR, Richartz RR, Gasino JM, Antunes J, Pinckney RD, Deconto I, Hoffmann DC, Thomaz-Soccol V (2006) Investigation of Neospora sp. and Toxoplasma gondii antibodies in mares and in precolostral foals from Parana State, Southern Brazil. Vet Parasitol 135: 215-221.
  42. Machačová T, Bártová E, Di Loria A, Sedlák K, Guccione J, Fulgione D, Veneziano V (2013) Seroprevalence and risk factors of Neospora spp. in donkeys from Southern Italy. Vet Parasitol 198: 201-204.
  43. Manca R, Ciccarese G, Scaltrito D, Chirizzi D (2022) Detection of Anti-Neospora caninum Antibodies on Dairy Cattle Farms in Southern Italy. Vet Sci 9: 87
  44. Marsh AE, Barr BC, Packham AE, Conrad PA (1998) Description of a new neospora species (Protozoa: apicomplexa: sarcocystidae). J Parasitol 5: 84-91.
  45. Mazuz ML, Mimoun L, Schvartz G, Tirosh-Levy S, Savitzki I, Edery N, Blum SE, Baneth G, Pusterla N, Steinman A (2020) Detection of Neospora caninum infection in aborted equine fetuses in Israel. Pathogenes 9: 1-11.
  46. Mendoza-Morales LF, Lagorio V, Corigliano MG, Sánchez-López E, Ramos-Duarte VA, Clemente M, Sander VA (2022) Neosporosis in sheep: A systematic review and meta-analysis of global seroprevalence and related risk factors. Acta Trop 233: 106569.
  47. Moore DP, de Yaniz MG, Odeón AC, Cano D, Leunda MR, Späth EA, Campero CM (2007) Serological evidence of Neospora caninum infections in goats from La Rioja Province, Argentina. Small Rumin Res 73: 256-258.
  48. Moreira TR, Sarturi C, Stelmachtchuk FN, Andersson E, Norlander E, de Oliveira FL, Machado Portela J, Marcili A, Emanuelson U, Gennari SM, Minervino AH (2019) Prevalence of antibodies against Toxoplasma gondii and Neospora spp. in equids of Western Pará Brazil. Acta Trop 189: 39-45.
  49. Moreno B, Collantes-Fernández E, Villa A, Navarro A, Regidor-Cerrillo J, Ortega-Mora LM (2012) Occurrence of Neospora caninum and Toxoplasma gondii infections in ovine and caprine abortions. Vet Parasitol 187: 312-318.
  50. Müller N, Zimmermann V, Hentrich B, Gottstein B (1996) Diagnosis of Neospora caninum and Toxoplasma gondii infection by PCR and DNA hybridization immunoassay. J Clin Microbiol 34: 2850-2852.
  51. Nayeri T, Sarvi S, Moosazadeh M, Daryani A (2022) The Global Prevalence of Neospora caninum Infection in Sheep and Goats That Had an Abortion and Aborted Fetuses: A Systematic Review and Meta-Analysis. Front Vet Sci 9: 870904
  52. Nooruldeen MY, Jaafar SE, Salih AI (2021) Seroprevalence of Neospora caninum infections in cattle in Kirkuk province. Iraqi J Vet Sci 35: 331-334.
  53. Novoa MB, Soler JP, Cirone KM, Hecker YP, Valentini BS, Primo ME, Moore DP (2023) Use and comparison of serologic assays to detect anti-Neospora caninum antibodies in farmed red deer (Cervus elaphus). Vet Parasitol 313: 109839.
  54. Rahmani SS, Malekifard F, Tavassoli M (2022) Neospora caninum, a cause of abortion in donkeys (Equus asinus) in Iran. Parasitol Res 121: 367-372.
  55. Reichel MP, Ayanegui-Alcérreca MA, Gondim LF, Ellis JT (2013) What is the global economic impact of Neospora caninum in cattle - the billion dollar question. Int J Parasitol 43: 133-142.
  56. Rodrigues AA, Brito DR, Kono IS, Reis SS, de Souza Lima Nino B, Nascimento TV, Barros LD, Garcia JL, de Cunha IA (2022) Seroprevalence of Neospora caninum and risk factors associated with infection in water buffaloes (Bubalus bubalis) from Maranhão State, Brazil. Vet Parasitol Reg Stud Reports 27: 100661.
  57. Sedlák K, Bartova E, Machacova T (2014) Seroprevalence of Neospora caninum in cats from the Czech Republic. Acta Parasitol 59: 359-361.
  58. Špilovská S, Reiterová K (2008) Seroprevalence of Neospora caninum in aborting sheep and goats in the Eastern Slovakia. Folia Vet 52: 33-35.
  59. Tayyub M, Ali S, Javid A, Imran M (2022) Molecular detection of Toxoplasma gondii and Neospora caninum in rock pigeons (Columba livia) in Punjab, Pakistan. Parasitol Res 121: 1499-1505
  60. Tirosh-Levy S, Savitsky I, Blinder E, Mazuz ML (2022) The involvement of protozoan parasites in sheep abortions – a ten-year review of diagnostic results. Vet Parasitol 303: 109664.
  61. Tirosh-Levy S, Steinman A, Minderigiu A, Arieli O, Savitski I, Fleiderovitz L, Edery N, Schvartz G, Mazuz ML (2020) High Exposure to Toxoplasma gondii and Neospora Spp. in Donkeys in Israel: Serological Survey and Case Reports. Animals 10: 1921
  62. Waap H, de Oliveira UV, Nunes T, Gomes J, Gomes T, Bärwald A, Dias Munhoz A, Schares G (2020) Serological survey of Neospora spp. and Besnoitia spp. in horses in Portugal. Vet Parasitol Reg Stud Reports 20: 100391.
  63. Wouda W, van den Ingh TS, van Knapen F, Sluyter FJ, Koeman JP, Dubey JP (1992) Neospora abortion in cattle in The Netherlands. Tijdschr Diergeneeskd 117: 599-602.
  64. Yang J, Ai J, Qi T, Ni X, Xu Z, Guo L, Sun Y, Li Y, Kang M, Li J (2022) Toxoplasma gondii and Neospora caninum Infections in Stray Cats and Dogs in the Qinghai-Tibetan Plateau Area, China. Animals 12: 1390
  65. Zhao SS, Tao DL, Chen JM, Chen X, Geng XL, Wang JW, Yang X, Song JK, Liu Q, Zhao GH (2022) Neospora caninum infection activated autophagy of caprine endometrial epithelial cells via mTOR signaling. Vet Parasitol 304: 109685.
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Authors and Affiliations

R.R. Mohammed
1
M. Tavassoli
1
K.R. Sidiq
2
B. Esmaeilnejad
1

  1. Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Nazloo Campus, PO Box 1177, Urmia, West Azerbaijan, Iran
  2. Department of Medical Laboratory Science, College of Medical and Applied Sciences, Charmo University, 46023 Chamchamal, Sulaimani, Kurdistan Region, Iraq
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Abstract

The present study was aimed to investigate oxidative stress, DNA damage, and histopatholog- ical alterations in hepatic tissues of splenectomized Wistar rats experimentally infected with Ba- besia bigemina. Rats were challenged with 5x106 infected erythrocytes. Babesia infection was con- firmed both with Giemsa’s staining blood smears and nested-PCR amplified region of apical membrane antigen-1 (AMA-1) gene. Parasitemia reached approximately 10 % at day 5 post-in- fection. Livers of infected rats were enlarged and darker in color, became extremely brittle with marked congestion. Microscopic evaluation showed cytoplasmic clearing of hepatocytes and se- vere hydropic changes with significantly dilated sinusoids containing macrophages and also intra- sinosoidal parasitized erythrocytes. Severe infiltration of lymphoplasma cells was also present throughout the liver parenchyma. Furthermore, Kupffer cells were enlarged and, occasionally, containing Babesia-parasitized erythrocytes. The activity of Glutathione (GSH) and catalase (CAT), and total antioxidant capacity (TAC) were also significantly decreased (p < 0.05) after infection of rats with B. bigemina. B. bigemina infection also induced a significant increase (p < 0.05) in hepatic malondialdehyde (MDA) and nitric oxide-derived products (NOx) concentra- tions as well as amount of endogenous hepatocytes DNA damage. Hepatic damage was also re- flected through the measurement of lactic acid dehydrogenase (LDH) and protein carbonyl con- tent (PCO) in liver cells. These two indices of liver injury were also significantly elevated (p < 0.5) during B. bigemina infection. Evaluation of correlation between assayed variables in infected rats revealed that MDA levels were positively correlated with PCO, NOx, LDH and DNA damage in the infected group and negatively correlated with GSH, CAT and TAC. There was also an inverse relationship between the antioxidant enzymes activities of GSH, CAT and TAC with PCO, NOx and DNA damage in infected rats. However, NOx showed positive correlation with PCO and DNA damage in infected rats. On the basis of the above results it can be concluded that the Ba- besia infection increases oxidative stress markers, protein carbonyl content and DNA damage and decreases antioxidant enzymes activities in the liver. These results suggest that B. bigemina infec- tion could alter the liver histopathology and causes DNA damage following oxidative stress in hepatic tissue. Further studies are needed to precisely define how hepatic tissue damage takes place in B. bigemina infection.

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Authors and Affiliations

B. Esmaeilnejad
M. Tavassoli
A. Samiei
A. Abbasi
A. Shafipour
N. Esmaeilnejad

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