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Number of results: 4
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Abstract

Previous studies indicated that particle size distribution affects the composition of keratinolytic and keratinophilic fungi in sewage sludge. The present study was to determine the composition of these fungi in sludge particle size fractions (> I, 1-0.5, 0.5--0.25, 0.25--0.125, 0.125-0.063, O 063--0.032 and < 0.032 mm) in a model experiment. I n the original sludge sample and its fractions, the composition of keratinolytic and kcratinophilic fungi was determined by using the hair baiting method. The composition of actidioneresistant fungi was also determined by using the dilution method and the Wiegand medium supplemented with chloramphenicol ( I 00 mg/dm') and actidione (500 mg/dm3). The number of keratinolytic and keratinophilic fungi isolated by the hair baiting method was lower in fractions than in the original sludge sample. In contrast, fungal quantities obtained by the dilution method were higher in fractions than in the original sludge sample. Qualitative differences were also observed. The conclusion was that nutrient factors associated with sludge particle size fractions, chietly total sulfur content and C:S ratio, affected the composition of keratinolytic and keratinophilic fungi in the sludge more than the fungal propagule quantities (inocula).
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Authors and Affiliations

Krzysztof Ulfig
Grażyna Plaza
Maciej Terakowski
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Abstract

The study was to determine the effect of water activity (0.850; 0.900; 0.950; 0.995; and 0.999 aw) on the growth of T. lanuginosus on solid media containing different cellulose substrates (crystalline cellulose, carboxymethyl cellulose - CMC, fi lter paper, and sawdust) and xylan. The growth of isolates from coffee beans and garden composts were compared. All isolates did not grow on media with aw < 0.950. On media with aw > 0.950, the hydrolysis zones were only observed on xylan and CMC. The highest daily growth and hydrolysis zone rates were mostly obtained at 0.995 aw and the lowest values were observed at 0.950 aw. The coffee beans isolates at 0.950 aw had the CMC hydrolysis coeffi cient 1.7-times higher than that for xylan. The fungal growth (FG) coeffi cient data indicate that the coffee beans isolates were able to utilize CMC and crystalline cellulose for growth and the highest growth rate was obtained at 0.999 aw. Subsequently, the compost isolates were able to grow on all substrates but the highest growth rate was obtained on CMC at 0.950 and 0.999 aw. Thus, coffee beans and composts provide T. lanuginosus isolates with various growth and hydrolytic zone rates in the range of 0.950−0.999 aw.
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Authors and Affiliations

Agata Markowska-Szczupak
Krzysztof Ulfig
Katarzyna Janda
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Abstract

The aim of this work was to determine the influence of native and alien keratinolytic fungi inocula on the petroleum hydrocarbon removal rate in soil covered and not covered with hair. The hair was the major nutrient for keratinolytic fungi and other soil microorganisms. The fungal inocula accelerated the petroleum hydrocarbon biodegradation process during the first month of the experiment. During the second month, TPH removal rates were similar for soil inoculated and not inoculated with fungi. The highest petroleum hydrocarbon removal rate was observed in soil inoculated with native fungal strains. The TPI-I removal rates were about 64% in soil covered with hair and 77% in soil not covered with hair. The lowest removal rate was observed in soil not inoculated with fungi (60%). The hair applied as additional nitrogen, sulfur and carbon source did not impact, or slightly inhibited, the petroleum hydrocarbon biodegradation process. The fungal inocula caused dramatic changes in soil fungal qualitative composition.
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Authors and Affiliations

Wioletta Przystaś
Krzysztof Ulfig
Korneliusz Miksch
Aleksandra Witała
Jerzy Szdzuj
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Abstract

TCE artificially contaminated soil was cleaned under anaerobic, reductive conditions. A laboratory scale treatability studies were carried out to determine optimal physico-chcmical and microbiological parameters for biorcmcdiation process. Upon treatability studies results a sewage sludge mixture was chosen as a microorganism's source. The chlorinated solvents contaminated soil bioreactor (CSCS bioreactor) was designed and built. It consists of a 6 m3 reactor vessel, a gas recirculation system, a leachate recirculation system and a data acquisition system. The bioreactor vessel was designed as a continuous gas flow packed bed reactor. During 210 days 4 Mg of soil containing approximately 350 mg TCE/kg of soil has been completely remediated under anaerobic conditions. The obtained results indicate that the stepwise dechlorination of TCE to ETH occurs in the bioreactor. Increasing amounts of chloride in the leachate were correlated with dechlorination.
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Authors and Affiliations

Adam Worsztynowicz
Dorota Rzychoń
Tomasz Siobowicz
Sebastian lwaszenko
Grażyna Płaza
Krzysztof Ulfig

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