The aim of this research was to determine the embryotoxic and teratogenic effects and lethal dose (LD50) of maropitant in ovo, using fertile chicken eggs. The study was designed in two stages, CHEST-I and CHEST-II. For CHEST-I, 210 fertile eggs were divided into seven equal groups; control, saline solution and 5 different doses of maropitant (10, 5, 2.5, 1.25, 0.625 mg/kg) injected groups. For CHEST-II, 150 fertile eggs were divided into five equal groups; control, saline solution and 3 different doses of maropitant (8, 6, 4 mg/kg)-injected groups. Eggs were opened on day 21 of incubation. Maropitant did not cause teratogenicity at any dose, while higher embryonic death rates were observed at doses above 4 mg/kg. The LD50 dose of maropitant was determined as 7.24 mg/kg. In conclusion, maropitant should only be used after full consideration of risks and benefits in pregnancy.

Brucellosis is a worldwide zoonosis, that can still be classified as endemic despite its ancient origins which causes economic losses and public health problems. Although effectively controlled by vaccination in animals, there is currently no vaccine for use in humans. Outer Membrane Proteins (OMP) that play an active immunogenic and protective role in the Brucellae family. OMP19 is present in all Brucella species as a surface antigen and is a potent immunogen responsible for Brucellosis intracellular infection. For this reason, the study was aimed to be used safely as a potential recombinant vaccine candidate against all Brucella infections, especially in humans and pregnant animals. This study evaluated a Brucella lipoprotein antigen, i.e. 19 kilodalton (kDa) outer membrane protein (OMP19), which was amplified and cloned into the pETSUMO vector system. The immunogenic power of the purified recombinant OMP19 antigen against brucellosis was compared with that of OMP19 (Raybiotech Inc, USA) in a mouse model and the obtained rOMP19 antigen was found to be similar to the commercially available recombinant protein.

Material suppliers typically recommend different additive amounts and applications for foundry practices. Therefore, even in the production of the same standard materials, different results may be obtained from various production processes on different foundry floors. In this study, the liquid metal prepared with the addition of different proportions of a FeSi-based inoculation, which is most commonly used in foundries in the production of a cast iron material with EN-GJL-250 lamellar graphite cast iron, was cast into sand molds prepared with a model designed to provide different solidification times. In this way, the optimization of the inoculation amounts on the casting structure for different solidification times was investigated. In addition, hardness values were determined depending on solidification time in varying amounts of inoculation additions. SolidCast casting simulation software was used to determine the casting model geometry and solidification time. In the scope of the study, sand casting, modeling, microstructure analysis, image analysis, microstructure analysis, and hardness tests techniques were used. When the results are examined, the required amount of inoculation for the optimal structure is optimized for the application procedure depending on the casting module and the solidification time.

Aluminum alloys are widely used in the industry thanks to its many advantages such as light weight and high strength. The use of this material in the market is increasing day by day with the developing technology. Due to the high energy inputs in the primary production, the use of secondary ingots by recycling from scrap material are more advantageous. However, the liquid metal quality is quite important in the use of secondary aluminum. It is believed that the quality of recycled aluminum is low, for this purpose, many liquid metal cleaning methods and test methods are used in the industry to assess the melt cleanliness level. In this study, it is aimed to examine the liquid metal quality in castings with varying temperature using K mold. A206 alloy was used, and the test parameters were selected as: (i) at 725 °C, 750 °C and 775 °C casting temperatures, (ii) different hydrogen levels. The hydrogen level was adjusted as low, medium and high with degassing, as-cast, and upgassing of the melt, respectively. The liquid metal quality of the cast samples was examined by the K mold technique. When the results were examined, it was determined that metal K values and the number of inclusions were high at the as-cast and up-gas liquid with increasing casting temperatures. It has been understood that the K mold technique is a practical method for the determination of liquid metal quality, if there is no reduced pressure test machine available at the foundry floor.