How to search?
advanced search

Search results

Filters

  • Journals
  • Authors
  • Keywords
  • Date
  • Type

Search results

Number of results: 2
items per page: 25 50 75
Sort by:

Antibiofilm activity against Staphylococcus aureus and content analysis of Taraxacum Officinale phenolic extract

P. Xu X.B. Xu A. Khan T. Fotina S.H. Wang
Polish Journal of Veterinary Sciences | 2021 | vol. 24 | No 2 | 243-251 | DOI: 10.24425/pjvs.2021.137659
Keywords Taraxacum Officinale phenolic antibacterial mechanism HPLC Staphylococcus aureus
Download PDF Download RIS Download Bibtex

Abstract

Taraxacum Officinale, commonly called dandelion, is herbaceous perennial belonging to the family of Asteraceae, having good antibacterial effects which are related to its phenolic substances. In this study, the effect of phenolic contents as well as the antibiofilm activity against Staphylococcus aureus of phenolic extract from T. Officinale were evaluated in vitro. With 70% metha- nol-water (v/v) as a solvent, the dandelion was extracted by ultrasonic assisted extraction method. Subsequent identification and quantification of phenol in extract was carried out using High Performance Liquid Chromatography (HPLC). The minimum inhibitory concentration and anti- bacterial kinetic curve of dandelion phenolic extract were analyzed by spectrophotometry. Changes in extracellular alkaline phosphatase (AKP) contents, electrical conductivity, intracellular protein contents, and DNA of S. aureus after the action of dandelion phenolic extract were determined to study its effect on the permeability of S. aureus cell wall and cell membrane. The results showed that chlorogenic acid (1.34 mg/g) was present in higher concentration, followed by luteolin (1.08 mg/g), ferulic acid (0.22 mg/g), caffeic acid (0.21 mg/g), and rutin (0.19 mg/g) in the dandelion phenolic extract. The minimum inhibitory concentration (MIC) of dandelion phenolic extract against S. aureus was 12.5 mg/mL. The antibacterial kinetic curve analysis showed that the inhibitory effect of dandelion phenolic extract on S. aureus was mainly in the exponential growth phase. After applying the dandelion phenolic extract, the growth of S. aureus was signifi- cantly inhibited entering into the decay phase early. Furthermore, after the action of dandelion, the extracellular AKP contents of S. aureus, the electrical conductivity and the extracellular protein contents were all increased. The phenolic extract also affected the normal reproduction of S. aureus. These results suggest that dandelion has an inhibitory effect on S. aureus, and the mechanism of its action was to destroy the integrity of the cell walls and cell membranes.
Go to article

Authors and Affiliations

P. Xu
1 2 3
X.B. Xu
1
A. Khan
4
T. Fotina
3
S.H. Wang
2
  1. School of Life Science and Basic Medicine, Xinxiang University, Jinsui St. 191, 453003 Xinxiang, China
  2. College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Eastern Hua Lan Street, 453003 Xinxiang, China
  3. Department of Veterinary Medicine, Sumy National Agrarian University, Herasima Kondratieva St. 160, 40021 Sumy, Ukraine
  4. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, P.R. China

Sire pedigree error estimation and sire verification of the Taiwan dairy cattle population by using SNP markers

C.H. Chao Y.H. Yeh Y.M. Chen K.H. Lee S.H. Wang T.Y. Lin
Polish Journal of Veterinary Sciences | 2022 | vol. 25 | No 1 | 61-65 | DOI: 10.24425/pjvs.2022.140841
Keywords Holstein cattle genetic testing sire pedigree
Download PDF Download RIS Download Bibtex

Abstract

Information regarding the correct pedigree of and relationship between animals is useful for managing dairy breeding, reducing inbreeding, estimating breeding value, and establishing correct breeding programs. Additionally, the successful implementation of progeny testing is crucial for improving the genetics of dairy cattle, which depends on the availability of correct pedigree information. Incorrect pedigree information leads to bias in bull evaluation. In this study, Neogen GeneSeek Genomic Profiler (GGP) 50K SNP chips were used to identify and verify the sire of Taiwanese Holstein dairy cattle and analyze the reasons that lead to incorrect sire records. Samples were collected from 2,059 cows of 36 dairy farms, and the pedigree information was provided by breeders. The results of sire verification can be divided into three categories: submitted unconfirmed sire, submitted confirmed sire, and incorrectly submitted verified sire. Data on the sires of 1,323 (64.25%) and 572 (27.78%) dairy cows were verified and discovered, respectively. Sires of 1,895 (92.03%) dairy cattle were identified, which showed that the paternal pedigree of dairy cattle could be discovered and verified through genetic testing. An error-like analysis revealed that the data of 37 sires were incorrectly recorded because the bull’s NAAB code number was incorrectly entered into the insemination records: for 19 sires, the wrong bull was recorded because the frozen semen of a bull placed in the wrong storage tank was used, 6 had no sire records, and for 12 sires, the NAAB code of the correct bull was recorded but with a wrong stud code, marketing code, or unique number for the stud or breed. To reduce recorded sire error rates by at least 27.78%, automated identification of the mated bull must be adopted to reduce human error and improve dairy breeding management on dairy farms.
Go to article

Bibliography


Banos G, Wiggans GR, Powell RL (2001) Impact of paternity errors in cow identification on genetic evaluations and international comparisons. J Dairy Sci 84: 2523-2529.
Beechinor JG, Kelly EP (1987) Errors of identification amongst cattle presented as progeny of some bulls used in the artificial insemination service in Ireland. Ir Vet J 41: 348-353.
Bovenhuis H, van Arendonk JA (1991) Estimation of milk protein gene frequencies in crossbred cattle by maximum likelihood. J Dairy Sci 74: 2728-2736.
Chao CH, Chen YM, Lee KH (2020) Genotype Screening of Bovine Brachyspina in Taiwan Holstein Cows. Am J Anim Vet Sci 15: 206-210.
Chao CH, Chen IM, Yeh YH, Lee KH, Lin TY (2021) Simultaneous genetic screening of bovine leukocyte adhesion deficiency, complex vertebral malformation, deficiency of uridine monophosphate synthase, and mule foot in Holstein cows in Taiwan. Appr Poult Dairy Vet Sci 8: 787-790.
Christensen LG, Madsen P, Petersen J (1982) The influence of incorrect sire-identification on the estimates of genetic parameters and breeding values. Proc. 2nd World Congr. Genet. Appl. Livest. Prod., Madrid, Spain 7: 200-208.
Davis GP, DeNise SK (1998) The impact of genetic markers on selection. J Anim Sci 76: 2331-2339.
Geldermann H, Pieper U, Weber WE (1986) Effect of misidentification on the estimation of breeding value and heritability in cattle. J Anim Sci 63: 1759-1768.
Heaton MP, Harhay GP, Bennett GL, Stone RT, Grosse WM, Casas E, Keele JK, Smith TP, Chitko-McKown CG, Laegreid WW (2002) Selection and use of SNP markers for animal identification and paternity analysis in U.S. beef cattle. Mamm Genome 13: 272-281.
ICAR (2017) The ICAR guidelines for parentage verification and parentage discovery based on SNP genotypes are used as standards for parental identification. www.icar.org/Documents/GenoEx/ICAR%20Guidelines%20 for%20Parentage%20Verification%20and%20Parentage%20Discovery%20based%20on%20SNP.pdf
Illumina (2017) Infinium XT Assay. https://support.illumina.com//content/dam/illumina-support/documents/documentation//chemistry_documentation/infinium_assays/infinium-xt/infinium-xt-ht-reference-guide-1000000016440-01.pdf
Israel C, Weller JI (2000) Effect of misidentification on genetic gain and estimation of breeding value in dairy cattle populations. J Dairy Sci 83: 181-187.
Kim S, Misra A (2007) SNP genotyping: technologies and biomedical applications. Annu Rev Biomed Eng 9: 289-320.
Oliphant A, Barker DL, Stuelpnagel JR, Chee MS (2002) BeadArray Technology: Enabling an accurate, cost-effective approach to high-throughput genotyping. Biotechniques 32: S56-S61.
Ron M, Blanc Y, Band M, Ezra E, Weller JL (1996) Misidentification rate in the Israeli dairy cattle population and its implications for genetic improvement. J Dairy Sci 79: 676-681.
Stormont C (1967) Contribution of blood typing to dairy science progress. J Dairy Sci 50: 253-260.
Visscher PM, Woolliams JA, Smith D, Williams JL (2002) Estimation of pedigree errors in the UK dairy population using microsatellite markers and the impact on selection. J Dairy Sci 85: 2368-2375.
Werner FA, Durstewitz G, Habermann FA, Thaller G, Kramer W, Kollers S, Buitkamp J, Georges M, Brem G, Mosner J, Fries R (2004) Detection and characterization of SNPs useful for identity control and parentage testing in major European dairy breeds. Anim Genet 35: 44-49.
Go to article

Authors and Affiliations

C.H. Chao
1
Y.H. Yeh
1
Y.M. Chen
1
K.H. Lee
1
S.H. Wang
1
T.Y. Lin
1
  1. Hsinchu Branch, Livestock Research Institute, Council of Agriculture, Executive Yuan, 207-5, Bi-tou-mian, Wu-hoo village, Si-hoo Township, Miaoli County, Taiwan

This page uses 'cookies'. Learn more