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MICROSTRUCTURE AND IMPACT TOUGHNESS OF OFFSHORE STEEL

T.-C. Cheng C. Yu T.-C. Yang C.-Y. Huang H.-C. Lin R.-K. Shiue
Archives of Metallurgy and Materials | 2018 | vol. 63 | No 1 | DOI: 10.24425/118924
Keywords low-temperature impact toughness prior austenite grain size hardenability bainite
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Authors and Affiliations

T.-C. Cheng
C. Yu
T.-C. Yang
C.-Y. Huang
H.-C. Lin
R.-K. Shiue

Proposed genotype definition of Porcine sapelovirus

T. Yang Y. Lu L. Zhang
Polish Journal of Veterinary Sciences | 2021 | vol. 24 | No 2 | 307-312 | DOI: 10.24425/pjvs.2021.137667
Keywords Porcine sapelovirus genotype phylogenetic and genetic analyses
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Abstract

Conventionally, Porcine sapelovirus (PSV) has been considered to comprise a single geno- type, PSV-1; however, a potentially novel member of PSV was recently discovered. In the present study, we propose a genotype definition of PSV based on phylogenetic and genetic analyses of the polyprotein, P1, and VP1 genes of available PSV sequences. Two genotypes, with pro- posed names PSV-1 and PSV-2, were identified. Moreover, the cut-off values (number of diffe- rences per site between amino acid sequences) for the definition of genotypes were established to be 0.1115 (polyprotein), 0.176 (P1), and 0.272 (VP1). The findings of this study are expected to enrich knowledge of PSV classification.
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Authors and Affiliations

T. Yang
1
Y. Lu
1
L. Zhang
1
  1. College of Life Sciences and Resource Environment, Yichun University, Yichun, Jiangxi 336000, China

Caspase-1 inhibitor reduced the lung injury in a mouse model of pleuropneumonia caused by Actinobacillus pleuropneumoniae

Y. Zhang T. Yang F. Huang
Polish Journal of Veterinary Sciences | 2020 | vol. 23 | No 4 | 605-610 | DOI: 10.24425/pjvs.2020.135807
Keywords porcine contagious pleuropneumonia mice caspase-1 inhibitors caspase-1dependent pyroptosis
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Abstract

The pathogenesis of porcine contagious pleuropneumonia is poorly understood. In the present study, a mouse model of intranasal infection by Actinobacillus pleuropneumoniae (App) was used to examine lung inflammation. The pathogical results of lung tissues showed that App-infected mice showed dyspnea and anorexia, with severe damage by acute hemorrhage, and infiltration of eosinophils and lymphocytes, as well as increased expression of caspase-1 p20, interleukin (IL)-1β, IL-6, IL-8, IL-18 and tumor necrosis factor (TNF)-α. Caspase-1 inhibitors reduced both lung tissue damage and the expression of caspase-1 p20, IL-1β, IL-6, IL-8, TNF-α and IL-18 in infected mice. These findings suggest that the caspase-1 dependent pyroptosis involved in the pathogenesis of the mouse pleuropneumonia caused by App and the inhibition of caspase-1 reduced the lung injury of this pleuropneumonia

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Authors and Affiliations

Y. Zhang
T. Yang
F. Huang

Development of TaqMan-based real-time PCR assay based on the E1 genefor the quantitative detection of the Getah virus

A. Lin X. Hu S. Cui T. Yang Z. Zhang P. Li M. Guo Y. Lu
Polish Journal of Veterinary Sciences | 2023 | vol. 26 | No 1 | 21-28 | DOI: 10.24425/pjvs.2023.145003
Keywords Getah virus real-time PCR TaqMan detection
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Abstract

To develop a sensitive, specific, and rapid approach for the detection Getah virus (GETV), a set of primers targeting the conserved region of the E1 gene was created. The TaqMan-based real-time PCR method for GETV detection was developed by optimizing the reaction conditions. The method demonstrated excellent specificity, and amplification did not occur with the causative agents of all prevalent swine viral infections (CSFV, PRRSV, PRV, PEDV, PTV, and JEV), except GETV. Additionally, upon assessing the sensitivity of the method, the minimum detection limit for GETV was found to be 5.94 copies/μL, which is 10 times higher than that of the traditional PCR approach. Further, the intra- and inter-assay variation coefficients were less than 1%, demonstrating good repeatability. Moreover, GETV was found in 10 of the 20 field serum samples using real-time PCR but only in three of the samples using traditional PCR. Consequently, the first GETV TaqMan-based real-time PCR approach based on the E1 gene was developed for GETV pathogenic diagnoses, and this exhibited high specificity, sensitivity, and repeatability. This assay is practical for the pathogenic diagnosis and epidemiology of GETV.
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Authors and Affiliations

A. Lin
1
X. Hu
1
S. Cui
1
T. Yang
1
Z. Zhang
1
P. Li
1
M. Guo
1
Y. Lu
1
  1. College of Life Sciences and Resource Environment, Yichun University, No 576, Xuefu Road, Yuanzhou district, Yichun, Jiangxi, 336000, China

Antibody level comparison after porcine epidemic diarrhea vaccination via different immunization routes

C. Hu X. Xie D. Zhao H. Liu X. Liu T. Yang W. Sun
Polish Journal of Veterinary Sciences | 2024 | vol. 27 | No 1 | 143-146 | DOI: 10.24425/pjvs.2024.149342
Keywords porcine epidemic diarrhea IgG IgA neutralizing antibody
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Abstract

Porcine epidemic diarrhea (PED) is a disease extremely harmful to pig health. Intramuscular and Houhai acupoint injections are the main immunization routes to prevent and control PED. This study aimed to evaluate the efficacy of these two routes in pregnant sows based on serum IgG, IgA, and neutralizing antibody levels. PED virus (PEDV) immunoprophylaxis with live-attenuated and inactivated vaccines was administered. The vaccinations for the intramuscular injections elevated IgG and neutralizing antibody levels more than Houhai acupoint injections at most timepoints after immunization. However, the anti-PEDV IgA antibodies induced by vaccination with the two immunization routes did not differ significantly. In conclusion, intramuscular injections are better than Houhai acupoint injections for PEDV vaccination of pregnant sows.
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Bibliography

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2. Hsueh FC, Chang YC, Kao CF, Hsu CW, Chang HW (2020) Intramuscular immunization with chemokine-adjuvanted inactive porcine epidemic diarrhea virus induces substantial protection in pigs. Vaccines (Basel) 8:102.
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11. Xu W, Hu S (2021) Administration of infectious bursal disease vaccine in Houhai acupoint promotes robust immune responses in chickens. Res Vet Sci 142: 149-153.
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Authors and Affiliations

C. Hu
1
X. Xie
2
D. Zhao
3
H. Liu
1
ORCID: ORCID
X. Liu
4
T. Yang
5
W. Sun
6
  1. Pulike Biological Engineering Inc., Luoyang, Henan, 471000, China
  2. Yiyang Vocational and Technical College, Yiyang, Hunan, 413055, China
  3. College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan, 410128, China
  4. Xiangtan Center for Animal Disease Prevention and Control, Xiangtan, Hunan, 411104, China
  5. College of Life Sciences and Resource Environment, Yichun University, Yichun, Jiangxi, 336000, China
  6. Sinopharm Animal Health Corporation Ltd., Wuhan, Hubei, 430075, China

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