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Abstract

To keep genetic diversity, flowering plants have developed a self-incompatibility system, which can prevent self-pollination.

It has been reported that calcium concentration in pistil papilla cells was increased after self-pollination

in transformed self-incompatible Arabidopsis thaliana. In this study, we found that CML27 changed its expression

level for both mRNA and protein when compared to transcriptome and proteome. At the same time, CML27 was

expressed in the anther and pistil at a high level and reached up to 5-fold up-regulated expression in the pistil

at 1 h post-pollination when compared to 0 min. In order to find out potential proteins that may interact with

BoCML27, BoCML27 was expressed in and isolated from E. coli. After its co-incubation with Brassica oleracea

pistil proteins, the products were separated on SDS-PAGE gels. We found a specific band at the position between

130–180 kDa. Through LC-MS-MS (Q-TOF) analysis, eight proteins were identified from the band. The proteins

include 26S proteasome non-ATPase regulatory (26S), Phospholipase D, alpha 2 (PLDα2) involved in Ca2+ binding

and Coatomer subunit alpha-2-like (Coatomer) involved in vesicle mediated transport. All of these identified

proteins provide new insights for the self-incompatibility response in B. oleracea, specific for increasing Ca2+

concentration in pistil papilla cells.

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Authors and Affiliations

Xiao Ping Lian
Jing Zeng
He Cui Zhang
Xiao Hong Yang
Liang Zhao
Li Quan Zhu

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