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A nanocrystalline Ti alloy powder was fabricated using cryomilling. The grain size and lattice strain evolution during cryomilling were quantitatively analyzed using X-ray diffraction (XRD) based on the Scherrer equation, Williamson-Hall (W-H) plotting method, and size-strain (S-S) method assuming uniform deformation. Other physical parameters including stress and strain have been calculated. The average crystallite size and the lattice strain evaluated from XRD analysis are in good agreement with the result of transmission electron microscopy (TEM).

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Authors and Affiliations

M. Oh

H.K. Yu

J.-H. Lee

M.C. Oh

S.-H. Jung

B. Ahn

Pharmacokinetics of the recombinant ovine interferon-tau in lambs

J. Zhao H.Y. Yu Y. Zhao S.Q. Li X.L. Fu W. Zhou B.B. Xia M.L. Wang J. Chen

Polish Journal of Veterinary Sciences | 2019 | vol.22 | No 1 | 75-82 | DOI: 10.24425/pjvs.2018.125610

Keywords bioavailability ovine interferon-τ cytopathic effect inhibition assay pharmacokinetic study

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Abstract

In the current study, twenty lambs, aged 4 months, half male and half female, were classified into four groups, with five in each group. The experimental three groups of lambs were given intravenous (IV), intramuscular (IM) and subcutaneous (SC) administrations of recombinant ovine interferon-τ (roIFN-τ). The fourth group (normal control) of lambs was given normal saline injections in the same way. After administrations, blood samples were collected from the tested animals at different time points post injection, and the serum titers of roIFN-τ were measured using cytopathic effect (CPE) inhibition bioassay. The results of calculating pharmacokinetic (PK) parameters using DAS software showed that the PK characteristics of roIFN-τ through IV injection conformed to the two-compartment open model, whose half-life of distribution phases (T1/2α) was 0.33±0.034 h and the elimination half-life(T1/2β) was 5.01±0.24 h. However, the PK features of IM injection and SC injection of roIFN-τ conformed to the one compartment open model, whose Tmax were 3.11±0.26 h and 4.83±0.43 h, respectively, together with an elimination half life(T1/2β) of 9.11±0.76 h and 7. 43±0.58 h, and an absorption half-life (T1/2k(a)) of 1.13±0.31 h and 1.85±0.40 h, respectively. The bioavailability of roIFN-τ after IM administration reaches 73.57%, which is greater than that of SC administration (53.43%). These results indicate that the drug administration effect can be preferably obtained following a single dose IM administration of the roIFN-τ aqueous preparation. This study will facilitate the clinical application of roIFN-τ as a potential antiviral agent in future work.

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Authors and Affiliations

J. Zhao

H.Y. Yu

Y. Zhao

S.Q. Li

X.L. Fu

W. Zhou

B.B. Xia

M.L. Wang

J. Chen

Seroprevalence of antibodies to classical swine fever virus and porcine reproductive and respiratory syndrome virus in healthy pigs in Hunan Province, China

H. Yu L. Zhang Y. Cai Z. Hao Z. Luo T. Peng L. Liu N. Wang G. Wang Z. Deng Y. Zhan

Polish Journal of Veterinary Sciences | 2022 | vol. 25 | No 3 | 375-381 | DOI: 10.24425/pjvs.2022.142020

Keywords classical swine fever virus (CSFV) porcine reproductive and respiratory syndrome virus (PRRSV) pig seroprevalence antibody

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Abstract

Classical swine fever (CSF) and porcine reproductive and respiratory syndrome (PRRS) are responsible for major economic losses and represent a threat to the swine industry worldwide. Routine surveillance serology for CSF and PRRS viruses is critical to maintaining the health status of sow farms in Hunan Province, which is one of the top pig production provinces in China. The aim of our study was to investigate the serological statistics of CSF virus (CSFV) and PRRS virus (PRRSV) in Hunan Province. The cohort serum samples were collected from vaccinated and unvaccinated pigs. Our findings showed that the average rates of CSFV and PRRSV antibody seropositivity were 82.2% (95% CI: 80.1-84.3) and 84.8% (95% CI: 82.5-87.1), respectively, in the immunized group and that these rates were higher than those in the unvaccinated group (58.6% for CSFV and 47.8% for PRRSV). Additionally, the level of CSFV antibody in piglet serum declined gradually with age, whereas PRRSV-specific antibody level increased initially (1 to 2 weeks old) and then declined with age (2 to 4 weeks old). In summary, we investigated the difference in CSFV/PRRSV antibody levels among piglets at various weeks old (1 to 4 weeks) to further establish the duration of maternal immunity in piglets. In addition, routine monitoring of CSFV/PRRSV antibodies in immunized pigs was carried out to evaluate the efficacy of vaccination.

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Authors and Affiliations

H. Yu

L. Zhang

Y. Cai

Z. Hao

Z. Luo

T. Peng

L. Liu

N. Wang

G. Wang

Z. Deng

Y. Zhan

Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Research Center of Reverse Vaccinology (RCRV), and Laboratory of Functional Proteomics (LFP), College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan 410128, China
Yongzhou Animal Husbandry and Aquatic Affairs Center, Yongzhou, Hunan 425000, China
Dingcheng Animal Husbandry and Aquatic Affairs Center, Changde, Hunan 415100, China

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Tuning The Crystallographic Structure And Morphology Of Nanocrystalline CaB6 Films Deposited By DC Magnetron Sputtering

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Quantitative Analysis of Cryomilled Nanocrystalline Ti-6Al-4V Alloy Powder by X-Ray Diffraction

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Pharmacokinetics of the recombinant ovine interferon-tau in lambs

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Seroprevalence of antibodies to classical swine fever virus and porcine reproductive and respiratory syndrome virus in healthy pigs in Hunan Province, China

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