By providing the body with essential nutrients, colostrum plays an immune and immunostimulating function. Colostrum quality depends on multiple factors, including microbial presence. This study aimed to explore the effect of non-aureus staphylococci on colostrum quality. Physical and chemical properties, fatty acid profile of cow colostrum were determined. In our study, we identified three non- aureus staphylococci species in the colostrum: S. sciuri, S. xylosus and S. warneri. The percentage of dry matter in staphylococci positive and negative colostrum samples did not differ significantly. Contents of fat, protein, and lactose in the colostrum were similar. The content of butyric (С4:0) and capric (С10:0) acids was significantly higher in the colostrum fat from samples positive for non- aureus staphylococci. Total bacterial count was lower in non- aureus staphylococci positive samples, while pH increased. The percentage of β-casein was lower in colostrum with a positive culture for non- aureus staphylococci.