The aim of this work was to induce permanent birefringence both in typical liquid crystal cells and photonic crystal fibers (PCFs) by photo-polymerization. For this purpose three different liquid crystalline materials, namely E7, 5CB, and 6CHBT were combined with a mixture of RM257 monomer and a UV sensitive initiator with the percentage weight less than 10%. Due to the photo-polymerization process it was possible to achieve polymer-stabilized liquid crystal orientation inside LC cells and micro-sized cylindrical glass tubes. In particular, periodic change in spatial molecular orientation was achieved by selective photo-polymerization. Successful results obtained in these simple geometries allowed for the experimental procedure to be repeated in PCFs leading to locally-induced permanent birefringence in PCFs.