Barley phylloplane is seriously colonized by Drechslera graminea, the causal agent of leaf
stripe disease in the hos. The present study involved the elucidation of alterations induced
in the protein content of the host due to Drechslera infection. Naturally growing barley
plants were obtained from fields and Drechslera graminea was isolated and identified from
diseased plants’ leaves. After identification and preparation of the pure culture, the pathogen
was inoculated on plants grown under aseptic and controlled laboratory conditions.
Changes in the total soluble cytoplasmic proteins and defense enzymes of the host such
as polyphenol oxidase (PPO), peroxidase (POX), phenylalanine lyase (PAL) and tyrosine
ammonia lyase (TAL) were observed up to 5 h after inoculation. The results demonstrated
a significant effect of the pathogen on the cytoplasmic protein expression of the host as well
as in its defense system.
The rules and guidelines for integrated pest management specified in Annex III, sections 2
and 3, state “General principles of integrated pest management”: Harmful organisms must
be monitored by adequate methods and tools, where available. Such adequate tools should
include observations in the field as well as scientifically sound warnings, forecasting and
early diagnostic systems, where feasible, as well as advice from professionally qualified advisors.
As part of Multiannual Programs, the Institute of Plant Protection – NRI in Poznań
has been carrying out work and research for many years to develop or modify guidelines for
monitoring short- and long-term forecasting of pest occurrence on crops. These guidelines
are extremely helpful for farmers and advisers in determining the optimum date of chemical
control of pests on plants. Regularly revised and improved the guidelines deal with pests
which currently pose a threat to crops. They are developed according to the latest scientific
findings and are successfully promoted among professional users and agricultural advisors.
These guidelines are standardized to include descriptions of species, life cycles, symptoms
of damage/infestation of crops, methods of observation targeted at warning of the need
for plant protection treatments, and threshold values of harmfulness. All guidelines include
extensive photographic material. Guidelines for the monitoring of pests on orchard
plants, vegetables and others are prepared at the Institute of Soil Science and Plant Cultivation
− NRI in Puławy and the Institute of Pomology in Skierniewice. Guidelines for about
80 pests of crops are available for public use in the on-line Pest Warning System (Platforma
Sygnalizacji Agrofagów, www.agrofagi.com.pl).