Phylloplane microbes have been studied as strategic tools in management against plant pathogens. Non-pathogenic bacteria and fungi have been applied as crop protectants against various plant diseases. The present study aimed at evaluating the potentiality of Aspergillus niger spores in altering the activity of four key enzymes related to defense in tomato. The experiment was designed such that two groups of 50 tomato plants were considered: group 1 – sprayed with autoclaved distilled water (control) and group 2 – sprayed with A. niger spores. Spraying was carried out under aseptic conditions. The experimental parameters included analysis of the activity of peroxidase (POX), polyphenol oxidase (PPO), phenylalanine ammonia lyase (PAL) and tyrosine ammonia lyase (TAL) as well as expression of POX and PPO isoforms. The results demonstrated an inductive effect of A. niger on the activity of POX, PPO, PAL and TAL. Enhanced expression of POX and PPO isoforms was also observed. The results indicated that A. niger can be considered probiotic for the management of tomato against its phytopathogens.

It was expected that there would be a relationship between plant density and arrangement within soybean plantations and ground beetles due to changes of abiotic habitat conditions. The aim of this study was to determinate the effect of different plant arrangements of soybean plants on the abundance and species diversity of ground beetles (Coleoptera, Carabidae). The studies were conducted from 2015 to 2017 at the Experimental Research Station, Wrocław, Poland. The occurrence of beetles was examined on soybeans, growing in four different treatments: row spacing of 15 cm or 30 cm, and seeding density of 50 or 90 seeds per m2. The experiment was conducted in a split-plot design in four replicates. Ground beetles were collected with 16 pitfall traps, with one trap in the middle part of each plot. The obtained results show that the general number of ground beetles was similar between the treatments. Some minor effects were found in species number, which was higher in the lower row spacing treatment. Only less abundant species were significantly affected. The most abundant species in all years and treatments were Pseudoophonus rufipes, Harpalus affinis, Calathus fuscipes and Pterostichus melanarius. The abundance of the above-listed common ground beetle species did not differ significantly between treatments.

The role of the tea commodity in the economy of Indonesia is quite strategic. Various types of microorganisms in nature have been known to increase the benefit of the root function, suppress disease, and accelerate plant growth. This study aimed to determine the potential of indigenous bacteria (Azoto II-1, Acinetobacter sp., bacteria Endo-5, bacteria Endo-65 and Endo-76) on the growth of tea plants and their potential in increasing resistance to blister blight disease. The test of microbes’ potential effect on growth and blister blight was conducted in Gambung, West Java in an experimental field using a randomized block design (RBD) with six treatments and each treatment was replicated four times. The composition of the treatments was: A) Endo-5; B) Endo-65; C) Endo-76; D) Azoto II-1; E) Acinetobacter sp.; and F) control (without microbes). Bacterial suspension was applied directly to the soil at a dose of 2 l · ha−1. The bacterial suspension was applied six times at 1 week intervals. The results of field observations indicated that the intensity of blister blight decreased in all treatments but did not significantly differ from the control. Meanwhile, the results of Acinetobacter sp. treatment in tea shoots was 17.26% higher than the control.