The paper presents selected results of studies connected with modeling of a biological object which could be used for simulation and measurements of the selected human tissues optical transmittance. The studies were performed for transilluminated homogeneous tissue layers as well as for objects consisted of different tissues. During simulations the software built with LabVIEW environment was used. Experimental verification of the model structure was made with spectrophotometry. The presented examples of modeling concern the transmittance spectra for two selected specific objects: the venous blood and muscle tissue analyzed in the wavelength range extending from 360 nm to 900 nm. The implemented model could be used in estimating the content and thickness of particular layers distinguished in a complex object and prediction of their transillumination efficiency.