The research on impact of Cleonus piger Scop. (Coleoptera; Curculionidae) feeding and the occurrence of other pest insects on milk thistle plants grown in monoculture and crop rotation after cereals, with two different seeding dates was carried out in the years 2003–2005. The infestation and density of C. piger larvae in roots of plants grown in monoculture increased with subsequent developmental phases and subsequent years of the experiment. Feeding resulted in the decrease in crop yield by 40% compared to the crop rotation treatment. In crop rotation stands, the infestation of milk thistle roots by C. piger larvae was 4–5 times lower at the final phase than in monoculture. Postponing seeding by three weeks led to the decrease of infestation and density of C. piger larvae, but the crop yield was lower than that from the earlyseeded stands. No other phytophagous species of economic importance were found.